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J. Biol. Chem., Vol. 277, Issue 2, 1203-1209, January 11, 2002

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On the Molecular Basis of the Thermal Sensitivity of an Escherichia coli topA Mutant^*

Yong Wang, A. Simon Lynch, Sue-Jane Chen^§, and James C. Wang^¶

From the Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138

Studies of two temperature-sensitive Escherichia coli topA strains AS17 and BR83, both of which were supposed to carry a topA amber mutation and a temperature-sensitive supD43,74 amber-suppressor, led to conflicting results regarding the essentiality of DNA topoisomerase I in cells grown in media of low osmolarity. We have therefore reexamined the molecular basis of the temperature sensitivity of strain AS17. We find that the supD allele in this strain had lost its temperature sensitivity. The temperature sensitivity of the strain, in media of all osmolarity, results from the synthesis of a mutant DNA topoisomerase I that is itself temperature-sensitive. Nucleotide sequencing of the AS17 topA allele and studies of its expected cellular product show that the mutant enzyme is not as active as its wild-type parent even at 30 °C, a permissive temperature for the strain, and its activity relative to the wild-type enzyme is further reduced at 42 °C, a nonpermissive temperature. Our results thus implicate an indispensable role of DNA topoisomerase I in E. coli cells grown in media of any osmolarity.

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