JBC Invitrogen Ultrasensitive Cytokine Assays

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Originally published In Press as doi:10.1074/jbc.M110417200 on November 9, 2001

J. Biol. Chem., Vol. 277, Issue 2, 1229-1234, January 11, 2002
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Molecular Cloning and Characterization of CAPER, a Novel Coactivator of Activating Protein-1 and Estrogen Receptors*

Dong-Ju Jung, Soon-Young NaDagger , Doe Sun Na, and Jae Woon Lee§

From the Department of Life Science, Pohang University of Science and Technology, Pohang 790-784 and the Department of Biochemistry, College of Medicine, University of Ulsan, Seoul 138-736, Korea

Transcriptional coactivators either bridge transcription factors and the components of the basal transcription apparatus and/or remodel the chromatin structures. We isolated a novel nuclear protein based on its interaction with the recently described general coactivator activating signal cointegrator-2 (ASC-2). This protein CAPER (for coactivator of activating protein-1 (AP-1) and estrogen receptors (ERs)) selectively bound, among the many transcription factors we tested, the AP-1 component c-Jun and the estradiol-bound ligand binding domains of ERalpha and ERbeta . Interestingly, CAPER exhibited a cryptic autonomous transactivation function that becomes activated only in the presence of estradiol-bound ER. In cotransfections, CAPER stimulated transactivation by ERalpha , ERbeta , and AP-1. Thus, CAPER may represent a more selective transcriptional coactivator molecule that plays a pivotal role for the function of AP-1 and ERs in vivo in conjunction with the general coactivator ASC-2.

* This work was supported by grants from the Ministry of Science and Technology (to D. S. N.), the Basic Science Research Institute of Pohang University of Science and Technology, and GenoCheck, Inc. (to J. W. L).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number AY061882.

Dagger Current address: Dept. of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114.

§ To whom correspondence should be addressed: Dept. of Life Science, Pohang University of Science and Technology, Pohang 790-784, Korea. Tel.: 82-54-279-2129; Fax: 82-54-279-8374; E-mail jaewoon@postech.ac.kr.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


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