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J. Biol. Chem., Vol. 277, Issue 2, 1284-1291, January 11, 2002
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From the Platelet-derived growth factor-BB (PDGF-BB) and
basic fibroblast growth factor (bFGF) are potent growth factors active
on many cell types. The present study indicates that they directly interact in vitro. The interaction was investigated with
overlay experiments, surface plasmon resonance experiments, and
solid-phase immunoassays by immobilizing one factor or the other and by
steady-state fluorescence analysis. The interaction observed was
specific, dose-dependent, and saturable, and the
bFGF/PDGF-BB binding stoichiometry was found to be 2:1.
KD1 for the first step
equilibrium and the overall KD values were found to
be in the nanomolar and in the picomolar range, respectively. Basic
FGF/PDGF-BB interaction was strongly reduced as a function of time of
PDGF-BB proteolysis. Furthermore, docking analysis suggested that the
PDGF-BB region interacting with bFGF may overlap, at least in part,
with the PDGF-BB receptor-binding site. This hypothesis was supported
by surface plasmon resonance experiments showing that an anti-PDGF-BB antibody, known to inhibit PDGF-BB binding with its receptor, strongly
reduced bFGF/PDGF-BB interaction, whereas a control antibody was
ineffective. According to these data, the observed
bFGF·PDGF-BB complex formation might explain, at least in
part, previous observations showing that PDGF-BB chemotactic and
mitogenic activity on smooth muscle cells are strongly inhibited in the
presence of bFGF.
Platelet-derived Growth Factor-BB and Basic Fibroblast
Growth Factor Directly Interact in Vitro with High
Affinity*
,, and
Laboratorio di Patologia Vascolare, Istituto
Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere
Scientifico, 00167 Roma, the § Dipartimento di Biochimica e
Biofisica, Seconda Università di Napoli, via Costantinopoli
16, 80138 Napoli, and the ¶ Istituto di Scienze
dell'Alimentazione, CNR, via Roma 52 A/C, 83100 Avellino, Italy
*
This work was supported in part by European Union Grant
BMH4-CT95-1160 and by Grant ASI I/R/31/00.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Laboratorio di
Patologia Vascolare, Istituto Dermopatico dell'Immacolata, via dei
Monti di Creta 104, 00167 Roma, Italy. Tel.: 39-06-66462431 or
39-06-66462433; Fax: 39-06-66-46-24-30; E-mail:
a.facchiano@idi.it.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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