HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 20, 17630-17637, May 17, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/20/17630    most recent M110178200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stapleton, M. R. Articles by Green, J. Search for Related Content PubMed PubMed Citation Articles by Stapleton, M. R. Articles by Green, J. Social Bookmarking                      What's this?

Interaction of the Salmonella typhimurium Transcription and Virulence Factor SlyA with Target DNA and Identification of Members of the SlyA Regulon^*

Melanie R. Stapleton, Valia A. Norte, Robert C. Read^§, and Jeffrey Green^¶

From  the Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom and the ^§ Division of Genomic Medicine, University of Sheffield Medical School, Sheffield S10 2RX United Kingdom

The SlyA protein from Salmonella typhimurium is a transcription factor that contributes to virulence. It is shown that a slyA mutant is attenuated in the presence of murine macrophages compared with the parent strain. Moreover, after growth in minimal medium, survival of the slyA mutant was reduced. Altered levels of flagellin (fliC), PagC, IroN, and outer membrane proteins suggest that the slyA mutation affects the surface properties of Salmonella. The isolated SlyA protein is a cofactor-free homodimer that recognizes five sites within the promoter region of the slyA gene. One of these sites contained a near perfect inverted repeat TTAGCAAGCTAA. The other four sites contained related sequences. Occupation of the SlyA sites in the slyA promoter prevented open-complex formation, consistent with the pattern of slyA::lacZ expression parental and slyA mutant strains. By combining the footprinting data with potential SlyA binding sites recovered from a pool of random DNA sequences, a consensus was defined and used to probe the NIH Salmonella unfinished genomes data base. These searches revealed the presence of consensus SlyA sites upstream of omp, ispA, xseB, slyA, and a gene encoding a protein with homology to a hemagglutinin. Accordingly, transcription of an omp::lacZ fusion was reduced in a slyA mutant. Given the difficulties in obtaining a comprehensive picture of intracellular gene expression, the definition of the DNA sequence recognized by a transcription factor (SlyA) that is essential for survival in the macrophage environment should allow a complete regulon of genes with altered expression upon exposure to macrophages to be determined once the S. typhimurium genome annotation is complete.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				H. Song, W. Kong, N. Weatherspoon, G. Qin, W. Tyler, J. Turk, R. Curtiss III, and Y. Shi Modulation of the Regulatory Activity of Bacterial Two-component Systems by SlyA J. Biol. Chem., October 17, 2008; 283(42): 28158 - 28168. [Abstract] [Full Text] [PDF]

				A. A. Larrea, I. M. Pedroso, A. Malhotra, and R. S. Myers Identification of two conserved aspartic acid residues required for DNA digestion by a novel thermophilic Exonuclease VII in Thermotoga maritima Nucleic Acids Res., October 1, 2008; 36(18): 5992 - 6003. [Abstract] [Full Text] [PDF]

				D. M. Stoebel, A. Free, and C. J. Dorman Anti-silencing: overcoming H-NS-mediated repression of transcription in Gram-negative enteric bacteria Microbiology, September 1, 2008; 154(9): 2533 - 2545. [Abstract] [Full Text] [PDF]

				J. C. Perez, T. Latifi, and E. A. Groisman Overcoming H-NS-mediated Transcriptional Silencing of Horizontally Acquired Genes by the PhoP and SlyA Proteins in Salmonella enterica J. Biol. Chem., April 18, 2008; 283(16): 10773 - 10783. [Abstract] [Full Text] [PDF]

				D. Corbett, H. J. Bennett, H. Askar, J. Green, and I. S. Roberts SlyA and H-NS Regulate Transcription of the Escherichia coli K5 Capsule Gene Cluster, and Expression of slyA in Escherichia coli Is Temperature-dependent, Positively Autoregulated, and Independent of H-NS J. Biol. Chem., November 16, 2007; 282(46): 33326 - 33335. [Abstract] [Full Text] [PDF]

				W. W. Navarre, M. McClelland, S. J. Libby, and F. C. Fang Silencing of xenogeneic DNA by H-NS--facilitation of lateral gene transfer in bacteria by a defense system that recognizes foreign DNA Genes & Dev., June 15, 2007; 21(12): 1456 - 1471. [Abstract] [Full Text] [PDF]

				K. Wei, D.-J. Tang, Y.-Q. He, J.-X. Feng, B.-L. Jiang, G.-T. Lu, B. Chen, and J.-L. Tang hpaR, a Putative marR Family Transcriptional Regulator, Is Positively Controlled by HrpG and HrpX and Involved in the Pathogenesis, Hypersensitive Response, and Extracellular Protease Production of Xanthomonas campestris Pathovar campestris J. Bacteriol., March 1, 2007; 189(5): 2055 - 2062. [Abstract] [Full Text] [PDF]

				N. Okada, Y. Oi, M. Takeda-Shitaka, K. Kanou, H. Umeyama, T. Haneda, T. Miki, S. Hosoya, and H. Danbara Identification of amino acid residues of Salmonella SlyA that are critical for transcriptional regulation Microbiology, February 1, 2007; 153(2): 548 - 560. [Abstract] [Full Text] [PDF]

				C. von Rhein, K.-P. Hunfeld, and A. Ludwig Serologic Evidence for Effective Production of Cytolysin A in Salmonella enterica Serovars Typhi and Paratyphi A during Human Infection Infect. Immun., November 1, 2006; 74(11): 6505 - 6508. [Abstract] [Full Text] [PDF]

				C. M. Reynolds, A. A. Ribeiro, S. C. McGrath, R. J. Cotter, C. R. H. Raetz, and M. S. Trent An Outer Membrane Enzyme Encoded by Salmonella typhimurium lpxR That Removes the 3'-Acyloxyacyl Moiety of Lipid A J. Biol. Chem., August 4, 2006; 281(31): 21974 - 21987. [Abstract] [Full Text] [PDF]

				S. A. Linehan, A. Rytkonen, X.-J. Yu, M. Liu, and D. W. Holden SlyA Regulates Function of Salmonella Pathogenicity Island 2 (SPI-2) and Expression of SPI-2-Associated Genes Infect. Immun., July 1, 2005; 73(7): 4354 - 4362. [Abstract] [Full Text] [PDF]

				M.-J. Ferrandiz, K. Bishop, P. Williams, and H. Withers HosA, a Member of the SlyA Family, Regulates Motility in Enteropathogenic Escherichia coli Infect. Immun., March 1, 2005; 73(3): 1684 - 1694. [Abstract] [Full Text] [PDF]

				Y. Shi, T. Latifi, M. J. Cromie, and E. A. Groisman Transcriptional Control of the Antimicrobial Peptide Resistance ugtL Gene by the Salmonella PhoP and SlyA Regulatory Proteins J. Biol. Chem., September 10, 2004; 279(37): 38618 - 38625. [Abstract] [Full Text] [PDF]

				C. Rouanet, S. Reverchon, D. A. Rodionov, and W. Nasser Definition of a Consensus DNA-binding Site for PecS, a Global Regulator of Virulence Gene Expression in Erwinia chrysanthemi and Identification of New Members of the PecS Regulon J. Biol. Chem., July 16, 2004; 279(29): 30158 - 30167. [Abstract] [Full Text] [PDF]

				N. R. Wyborn, M. R. Stapleton, V. A. Norte, R. E. Roberts, J. Grafton, and J. Green Regulation of Escherichia coli Hemolysin E Expression by H-NS and Salmonella SlyA J. Bacteriol., March 15, 2004; 186(6): 1620 - 1628. [Abstract] [Full Text] [PDF]

				V. A. Norte, M. R. Stapleton, and J. Green PhoP-Responsive Expression of the Salmonella enterica Serovar Typhimurium slyA Gene J. Bacteriol., June 15, 2003; 185(12): 3508 - 3514. [Abstract] [Full Text] [PDF]