JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M106101200 on February 5, 2002

J. Biol. Chem., Vol. 277, Issue 20, 17962-17969, May 17, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/20/17962    most recent
M106101200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shariat-Madar, Z.
Right arrow Articles by Schmaier, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shariat-Madar, Z.
Right arrow Articles by Schmaier, A. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Identification and Characterization of Prolylcarboxypeptidase as an Endothelial Cell Prekallikrein Activator*

Zia Shariat-MadarDagger , Fakhri MahdiDagger , and Alvin H. SchmaierDagger §

From the Division of Hematology and Oncology, Departments of Dagger  Internal Medicine and § Pathology, University of Michigan, Ann Arbor, Michigan 48109-0640

Our recent investigations have postulated a human umbilical vein endothelial cell (HUVEC)-associated prekallikrein activator (PKA). When prekallikrein (PK) assembles on high molecular weight kininogen on HUVEC, PK is activated to kallikrein. PKA was found in the 15,800 × g pellet of HUVEC lysates using an assay that measures PK activation only when bound to high molecular weight kininogen linked to microtiter plates. Sequential DEAE, wheat germ lectin affinity, and hydroxyapatite chromatography resulted in four protein bands on SDS-PAGE. One protein in the 73-kDa band was identified by amino acid sequencing as prolylcarboxypeptidase (PRCP). On gel filtration, PKA activity was a single homogenous peak identical in migration to the 73-kDa immunoblot of PRCP. Anti-PRCP inhibits PKA activity and PK activation on HUVEC. Purified PKA was blocked by diisopropyl fluorophosphate (1 mM), phenylmethylsulfonyl fluoride (3 mM), leupeptin (100 µM), antipain (IC50 = 2 µM), HgCl2 (IC50 = 500 µM), Z-Pro-Pro-aldehyde-dimethyl acetate (IC50 = 1 µM), and corn trypsin inhibitor (IC50 = 40 nM). PKA did not correct the coagulant defect in factor XII deficient plasma, was purified from HUVEC cultured in factor XII-deficient serum, was not detected by antibody to factor XII, did not activate FXI, and was not inhibited by a neutralizing antibody to FXII. Angiotensin II (IC50 = 2 µM) or bradykinin (IC50 = 100 µM), but not angiotensin II-(1-7) or bradykinin1-5, and the prolyl oligopeptidase inhibitor Fmoc-Ala-Pyr-CN (IC50 = 50 nM) also blocked purified PKA activation of PK. The Km of PK activation by PRCP is 6.7 nM. PRCP antigen is present on the membrane of fixed but not permeabilized HUVEC. PRCP appears to be a HUVEC-associated PK activator.

* This work was supported by National Institutes of Health Grant HL52779.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Internal Medicine, University of Michigan, 5301 MSRB III, 1150 W. Medical Center Dr., Ann Arbor, MI 48109-0649. Tel.: 734-647-3124; Fax: 734-647-5669; E-mail: aschmaie@umich.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
Z. Shariat-Madar, F. Mahdi, M. Warnock, J. W. Homeister, S. Srikanth, Y. Krijanovski, L. J. Murphey, A. A. Jaffa, and A. H. Schmaier
Bradykinin B2 receptor knockout mice are protected from thrombosis by increased nitric oxide and prostacyclin
Blood, July 1, 2006; 108(1): 192 - 199.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Tang, C. L. Yu, S. R. Williams, E. Springman, D. Jeffery, P. A. Sprengeler, A. Estevez, J. Sampang, W. Shrader, J. Spencer, et al.
Expression, Crystallization, and Three-dimensional Structure of the Catalytic Domain of Human Plasma Kallikrein
J. Biol. Chem., December 9, 2005; 280(49): 41077 - 41089.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Z. Shariat-Madar, E. Rahimy, F. Mahdi, and A. H. Schmaier
Overexpression of prolylcarboxypeptidase enhances plasma prekallikrein activation on Chinese hamster ovary cells
Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2697 - H2703.
[Abstract] [Full Text] [PDF]

Home page
 ASH ANNUAL MEETING ABSTRACTSHome page
Z. Shariat-Madar, E. Rahimy, F. Mahdi, and A. H. Schmaier
Over-Expression of Prolylcarboxypeptidase Enhances Plasma Prekallikrein Activation on Chinese Hamster Ovary Cell Membranes.
Blood (ASH Annual Meeting Abstracts), November 16, 2005; 106(11): 3698 - 3698.
[Abstract]

Home page
 J BiochemHome page
N. Kato, T. Okayama, H. Isawa, M. Yuda, Y. Chinzei, and S. Iwanaga
Contribution of the N-Terminal and C-Terminal Domains of Haemaphysalin to Inhibition of Activation of Plasma Kallikrein-Kinin System
J. Biochem., September 1, 2005; 138(3): 225 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. N. Fernando, L. P. Fernando, Y. Fukuda, and A. P. Kaplan
Assembly, activation, and signaling by kinin-forming proteins on human vascular smooth muscle cells
Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H251 - H257.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. S. Song, I. M. Sainz, S. C. Cosenza, I. Isordia-Salas, A. Bior, H. N. Bradford, Y.-L. Guo, R. A. Pixley, E. P. Reddy, and R. W. Colman
Inhibition of tumor angiogenesis in vivo by a monoclonal antibody targeted to domain 5 of high molecular weight kininogen
Blood, October 1, 2004; 104(7): 2065 - 2072.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
R. G. Espinola, A. Uknis, I. M. Sainz, I. Isordia-Salas, R. Pixley, R. DeLa Cadena, W. Long, A. Agelan, J. Gaughan, A. Adam, et al.
A Monoclonal Antibody to High-Molecular Weight Kininogen Is Therapeutic in a Rodent Model of Reactive Arthritis
Am. J. Pathol., September 1, 2004; 165(3): 969 - 976.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Z. Shariat-Madar, F. Mahdi, and A. H. Schmaier
Recombinant prolylcarboxypeptidase activates plasma prekallikrein
Blood, June 15, 2004; 103(12): 4554 - 4561.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Mahdi, Z. Shariat-Madar, A. Kuo, M. Carinato, D. B. Cines, and A. H. Schmaier
Mapping the Interaction between High Molecular Mass Kininogen and the Urokinase Plasminogen Activator Receptor
J. Biol. Chem., April 16, 2004; 279(16): 16621 - 16628.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Tan, F. N. Hutchison, and A. A. Jaffa
Mechanisms of angiotensin II-induced expression of B2 kinin receptors
Am J Physiol Heart Circ Physiol, March 1, 2004; 286(3): H926 - H932.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
Z. Shariat-Madar and A. H. Schmaier
Review: The plasma kallikrein/kinin and renin angiotensin systems in blood pressure regulation in sepsis
Innate Immunity, February 1, 2004; 10(1): 3 - 13.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
F. Mahdi, Z. Shariat-Madar, and A. H. Schmaier
The Relative Priority of Prekallikrein and Factors XI/XIa Assembly on Cultured Endothelial Cells
J. Biol. Chem., November 7, 2003; 278(45): 43983 - 43990.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. H. Schmaier
The kallikrein-kinin and the renin-angiotensin systems have a multilayered interaction
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2003; 285(1): R1 - R13.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. A. Skidgel, F. Alhenc-Gelas, and W. B. Campbell
Regulation of Cardiovascular Signaling by Kinins and Products of Similar Converting Enzyme Systems: Prologue: Kinins and related systems. New life for old discoveries
Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H1886 - H1891.
[Full Text] [PDF]

Home page
 BloodHome page
Y. Krijanovski, V. Proulle, F. Mahdi, M. Dreyfus, W. Muller-Esterl, and A. H. Schmaier
Characterization of molecular defects of Fitzgerald trait and another novel high-molecular-weight kininogen-deficient patient: insights into structural requirements for kininogen expression
Blood, June 1, 2003; 101(11): 4430 - 4436.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
A. A. Jaffa, R. Durazo-Arvizu, D. Zheng, D. T. Lackland, S. Srikanth, W. T. Garvey, and A. H. Schmaier
Plasma Prekallikrein: A Risk Marker for Hypertension and Nephropathy in Type 1 Diabetes
Diabetes, May 1, 2003; 52(5): 1215 - 1221.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.