Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M111640200 on March 4, 2002

J. Biol. Chem., Vol. 277, Issue 20, 18198-18205, May 17, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/20/18198    most recent
M111640200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bäckhed, F.
Right arrow Articles by Richter-Dahlfors, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bäckhed, F.
Right arrow Articles by Richter-Dahlfors, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Identification of Target Tissue Glycosphingolipid Receptors for Uropathogenic, F1C-fimbriated Escherichia coli and Its Role in Mucosal Inflammation*

Fredrik BäckhedDagger §, Björn Alsén§||, Niamh Roche||**, Jonas Ångström||, Anne von EulerDagger , Michael E. BreimerDagger Dagger , Benita Westerlund-Wikström§§, Susann Teneberg||¶¶, and Agneta Richter-DahlforsDagger ||||

From the Dagger  Microbiology and Tumorbiology Center, Karolinska Institute, SE 171 77 Stockholm, Sweden, the || Institute of Medical Biochemistry, Göteborg University, P. O. Box 440, SE 405 30 Göteborg, Sweden, the Dagger Dagger  Department of Surgery, Sahlgrenska University Hospital, SE 413 45 Göteborg, Sweden, and the §§ Division of General Microbiology, Department of Biosciences, P. O. Box 56, University of Helsinki, Helsinki FIN-00014 Finland

Bacterial adherence to mucosal cells is a key virulence trait of pathogenic bacteria. The type 1 fimbriae and the P-fimbriae of Escherichia coli have both been described to be important for the establishment of urinary tract infections. While P-fimbriae recognize kidney glycosphingolipids carrying the Galalpha 4Gal determinant, type 1 fimbriae bind to the urothelial mannosylated glycoproteins uroplakin Ia and Ib. The F1C fimbriae are one additional type of fimbria correlated with uropathogenicity. Although it was identified 20 years ago its receptor has remained unidentified. Here we report that F1C-fimbriated bacteria selectively interact with two minor glycosphingolipids isolated from rat, canine, and human urinary tract. Binding-active compounds were isolated and characterized as galactosylceramide, and globotriaosylceramide, both with phytosphingosine and hydroxy fatty acids. Comparison with reference glycosphingolipids revealed that the receptor specificity is dependent on the ceramide composition. Galactosylceramide was present in the bladder, urethers, and kidney while globotriaosylceramide was present only in the kidney. Using a functional assay, we demonstrate that binding of F1C-fimbriated Escherichia coli to renal cells induces interleukin-8 production, thus suggesting a role for F1C-mediated attachment in mucosal defense against bacterial infections.

* This work was supported in part by the Swedish Medical Research Council, the Swedish Cancer Foundation, and the Wallenberg Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Both authors contributed equally to this work.

Supported by grants from the program "Glycoconjugates in Biological Systems" sponsored by the Swedish Foundation for Strategic Research.

** Supported by grants from the program "Glycoconjugates in Biological Systems" sponsored by the Swedish Foundation for Strategic Research.

¶¶ To whom correspondence should be addressed: Institute of Medical Biochemistry, Göteborg University, P.O. Box 440, SE 405 30 Göteborg, Sweden. Tel.: 46-31-773-3492; Fax: 46-31-413-190; E-mail: Susann.Teneberg@medkem.gu.se.

|||| Recipient of special grants from The Royal Swedish Academy of Sciences and the Swedish Foundation for Strategic Research.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Coddens, M. Diswall, J. Angstrom, M. E. Breimer, B. Goddeeris, E. Cox, and S. Teneberg
Recognition of Blood Group ABH Type 1 Determinants by the FedF Adhesin of F18-fimbriated Escherichia coli
J. Biol. Chem., April 10, 2009; 284(15): 9713 - 9726.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Lindberg, Y. Xia, B. Sonden, M. Goransson, J. Hacker, and B. E. Uhlin
Regulatory Interactions among Adhesin Gene Systems of Uropathogenic Escherichia coli
Infect. Immun., February 1, 2008; 76(2): 771 - 780.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. Klemm, V. Hancock, and M. A. Schembri
Mellowing Out: Adaptation to Commensalism by Escherichia coli Asymptomatic Bacteriuria Strain 83972
Infect. Immun., August 1, 2007; 75(8): 3688 - 3695.
[Full Text] [PDF]

Home page
 MicrobiologyHome page
G. C. Ulett, A. N. Mabbett, K. C. Fung, R. I. Webb, and M. A. Schembri
The role of F9 fimbriae of uropathogenic Escherichia coli in biofilm formation
Microbiology, July 1, 2007; 153(7): 2321 - 2331.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
V. Roos, M. A. Schembri, G. C. Ulett, and P. Klemm
Asymptomatic bacteriuria Escherichia coli strain 83972 carries mutations in the foc locus and is unable to express F1C fimbriae
Microbiology, June 1, 2006; 152(6): 1799 - 1806.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. A. Snyder, B. J. Haugen, C. V. Lockatell, N. Maroncle, E. C. Hagan, D. E. Johnson, R. A. Welch, and H. L. T. Mobley
Coordinate Expression of Fimbriae in Uropathogenic Escherichia coli
Infect. Immun., November 1, 2005; 73(11): 7588 - 7596.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
I. P. Oswald, C. Desautels, J. Laffitte, S. Fournout, S. Y. Peres, M. Odin, P. Le Bars, J. Le Bars, and J. M. Fairbrother
Mycotoxin Fumonisin B1 Increases Intestinal Colonization by Pathogenic Escherichia coli in Pigs
Appl. Envir. Microbiol., October 1, 2003; 69(10): 5870 - 5874.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement