|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 21, 18586-18591, May 24, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the To identify the gene products responsible for the
calcium-activated chloride current in smooth muscle, reverse
transcription-PCR with degenerate primers was performed on mouse
intestine and other organs. A new member of the CLCA gene family was
identified, mCLCA4, that is expressed preferentially in organs
containing a high percentage of smooth muscle cells, including
intestine, stomach, uterus, bladder, and aorta. Reverse
transcription-PCR using template RNA prepared from mouse bladder and
stomach smooth muscle layers dissected free of mucosa yielded
mCLCA4-specific bands. In situ hybridization with an
mCLCA4-specific probe confirmed prominent expression in smooth muscle
of major vessels of the heart but not cardiac muscle. High expression
was also detected in the gastrointestinal tract, in bronchioles, and in
aortic and lung endothelial cells. Transient expression of mCLCA4 in
293T cells resulted in the appearance of a prominent calcium-activated
chloride current. Whole-cell currents activated by ionomycin or
methacholine were anion-selective and showed minimal rectification or
voltage-dependent gating. Similar to endogenous currents in
smooth muscle cells, methacholine-induced currents were transient, and
spontaneous transient inward currents were occasionally observed at
resting membrane potentials. These results link calcium-activated
chloride channels in smooth muscle with a gene family whose members
have been implicated in cystic fibrosis, cancer, and asthma.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AY00827.
Molecular and Functional Characterization of a Murine
Calcium-activated Chloride Channel Expressed in Smooth Muscle*
§,
,,
Cancer Biology Laboratories and Departments
of Molecular Medicine and ¶ Biomedical Sciences, Cornell
University College of Veterinary Medicine, Ithaca, New York 14853 and
Center for Oral Biology and ** Center for Human
Genetics and Molecular Pediatric Disease, Aab Institute for Biomedical
Research, University of Rochester,
Rochester, New York 14642
*
This work was supported by United States Army Breast Cancer
Research Fund Grant DAMD17-00-1-0219 (to R. C. E.), NCI, National Institutes of Health (NIH) Public Health Service (PBS) Grant CA47668 (to B. U. P.), PHS Grant DE09692 (to K. N.), and NIH PHS Grants HL45239, HL41084, and DK58795 (to M. I. K.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Song, X. Zhang, Z. Qi, G. Sun, S. Chi, Z. Zhu, J. Ren, Z. Qiu, K. Liu, L. Myatt, et al. Cloning and Characterization of a Calcium-Activated Chloride Channel in Rat Uterus Biol Reprod, April 1, 2009; 80(4): 788 - 794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Huan, K. S. Greene, B. Shui, G. Spizz, H. Sun, R. M. Doran, P. J. Fisher, M. S. Roberson, R. C. Elble, and M. I. Kotlikoff mCLCA4 ER processing and secretion requires luminal sorting motifs Am J Physiol Cell Physiol, July 1, 2008; 295(1): C279 - C287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Bothe, J. Braun, L. Mundhenk, and A. D. Gruber Murine mCLCA6 Is an Integral Apical Membrane Protein of Non-goblet Cell Enterocytes and Co-localizes With the Cystic Fibrosis Transmembrane Conductance Regulator J. Histochem. Cytochem., May 1, 2008; 56(5): 495 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. Elble, V. Walia, H.-c. Cheng, C. J. Connon, L. Mundhenk, A. D. Gruber, and B. U. Pauli The Putative Chloride Channel hCLCA2 Has a Single C-terminal Transmembrane Segment J. Biol. Chem., October 6, 2006; 281(40): 29448 - 29454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Mundhenk, M. Alfalah, R. C. Elble, B. U. Pauli, H. Y. Naim, and A. D. Gruber Both Cleavage Products of the mCLCA3 Protein Are Secreted Soluble Proteins J. Biol. Chem., October 6, 2006; 281(40): 30072 - 30080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J-W Jeong, K Y Lee, J P Lydon, and F J DeMayo Steroid hormone regulation of Clca3 expression in the murine uterus. J. Endocrinol., June 1, 2006; 189(3): 473 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nakano, H. Inoue, S. Fukuyama, K. Matsumoto, M. Matsumura, M. Tsuda, T. Matsumoto, H. Aizawa, and Y. Nakanishi Niflumic Acid Suppresses Interleukin-13-induced Asthma Phenotypes Am. J. Respir. Crit. Care Med., June 1, 2006; 173(11): 1216 - 1221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Robert, C. Norez, and F. Becq Disruption of CFTR chloride channel alters mechanical properties and cAMP-dependent Cl- transport of mouse aortic smooth muscle cells J. Physiol., October 15, 2005; 568(2): 483 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Loewen and G. W. Forsyth Structure and Function of CLCA Proteins Physiol Rev, July 1, 2005; 85(3): 1061 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Beckley, B. U. Pauli, and R. C. Elble Re-expression of Detachment-inducible Chloride Channel mCLCA5 Suppresses Growth of Metastatic Breast Cancer Cells J. Biol. Chem., October 1, 2004; 279(40): 41634 - 41641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Evans, W. B. Thoreson, and C. L. Beck Molecular and Functional Analyses of Two New Calcium-activated Chloride Channel Family Members from Mouse Eye and Intestine J. Biol. Chem., October 1, 2004; 279(40): 41792 - 41800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Boese, O. Aziz, N. L. Simmons, and M. A. Gray Kinetics and regulation of a Ca2+-activated Cl- conductance in mouse renal inner medullary collecting duct cells Am J Physiol Renal Physiol, April 1, 2004; 286(4): F682 - F692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Qu, R. Fischmeister, and C. Hartzell Mouse Bestrophin-2 Is a Bona fide Cl- Channel: Identification of a Residue Important in Anion Binding and Conduction J. Gen. Physiol., March 29, 2004; 123(4): 327 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wrayzx, K. Jones, S. Kupittayanant, Y. Li, A. Matthew, E. Monir-Bishty, K. Noble, S. J. Pierce, S. Quenby, and A. V. Shmygol Calcium Signaling and Uterine Contractility Reproductive Sciences, July 1, 2003; 10(5): 252 - 264. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Gustincich, S. Batalov, K. W. Beisel, H. Bono, P. Carninci, C. F. Fletcher, S. Grimmond, N. Hirokawa, E. D. Jarvis, T. Jegla, et al. Analysis of the Mouse Transcriptome for Genes Involved in the Function of the Nervous System Genome Res., June 1, 2003; 13(6): 1395 - 1401. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |