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J. Biol. Chem., Vol. 277, Issue 21, 18914-18918, May 24, 2002
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From the The Aft1 transcription factor regulates the iron
regulon in response to iron availability in Saccharomyces
cerevisiae. Aft1 activates a battery of genes required for iron
uptake under iron-starved conditions, whereas Aft1 function is
inactivated under iron-replete conditions. Previously, we have shown
that iron-regulated DNA binding by Aft1 is responsible for the
controlled expression of target genes. Here we show that this
iron-regulated DNA binding by Aft1 is not due to the change in the
total expression level of Aft1 or alteration of DNA binding activity.
Rather, nuclear localization of Aft1 responds to iron status, leading
to iron-regulated expression of the target genes. We identified the
nuclear export signal (NES)-like sequence in the AFT1 open reading
frame. Mutation of the NES-like sequence causes nuclear retention of
Aft1 and the constitutive activation of Aft1 function independent of
the iron status of the cells. These results suggest that the nuclear export of Aft1 is critical for ensuring iron-responsive transcriptional activation of the Aft1 regulon and that the nuclear import/export systems are involved in iron sensing by Aft1 in S. cerevisiae.
Subcellular Localization of Aft1 Transcription Factor Responds to
Iron Status in Saccharomyces cerevisiae*
§,,, and Department of Applied Molecular Biology,
Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto
606-8502, Japan and the ¶ Department of Life Style Studies, School
of Human Cultures, The University of Shiga Prefecture, Hikone,
Shiga 522-8533, Japan
*
This work was supported by grants from the Ministry of
Education, Culture, Science, and Technology of Japan (to Y. Y.-I.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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