Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M201728200 on March 15, 2002

J. Biol. Chem., Vol. 277, Issue 21, 19191-19197, May 24, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/21/19191    most recent
M201728200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Torihashi, S.
Right arrow Articles by Nakayama, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Torihashi, S.
Right arrow Articles by Nakayama, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Calcium Oscillation Linked to Pacemaking of Interstitial Cells of Cajal
REQUIREMENT OF CALCIUM INFLUX AND LOCALIZATION OF TRP4 IN CAVEOLAE*

Shigeko TorihashiDagger §, Toyoshi FujimotoDagger , Claudia Trost, and Shinsuke Nakayama||

From the Departments of Dagger  Anatomy and Cell Biology and || Cell Physiology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan and the  Institute für Pharmakologie und Toxikologie, Universität des Saarlandes, D-66421 Homburg, Germany

Interstitial cells of Cajal (ICC) are considered to be pacemaker cells in gastrointestinal tracts. ICC generate electrical rhythmicity (dihydropyridine-insensitive) as slow waves and drive spontaneous contraction of smooth muscles. Although cytosolic Ca2+ has been assumed to play a key role in pacemaking, Ca2+ movements in ICC have not yet been examined in detail. In the present study, using cultured cell clusters isolated from mouse small intestine, we demonstrated Ca2+ oscillations in ICC. Fluo-4 was loaded to the cell cluster, the relative amount of cytosolic Ca2+ was recorded, and ICC were identified by c-Kit immunoreactivity. We specifically detected Ca2+ oscillation in ICC in the presence of dihydropyridine, which abolishes Ca2+ oscillation in smooth muscles. The oscillation was coupled to the electrical activity corresponding to slow waves, and it depended on Ca2+ influx through a non-selective cation channel, which was SK&F 96365-sensitive and store-operated. We further demonstrated the presence of transient receptor potential-like channel 4 (TRP4) in caveolae of ICC. Taken together, the results infer that the Ca2+ oscillation in ICC is intimately linked to the pacemaker function and depends on Ca2+ influx mediated by TRP4.

* This work was supported by the Ministry of Education, Science, Sports, and Culture of Japan and by the Japan Clinical Study Group for the Esophago-cardiac Region. A preliminary account was presented at the 18th International Symposium on Gastrointestinal Motility, November 15-19, 2001, Madison, WI (Torihashi, S., Fujimoto, T., and Nakayama, S. (2001) Neurogastroenterol. Motil. 13, 437a).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed: Dept. of Anatomy and Cell Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-8550, Japan. Tel.: 81-52-744-2001; Fax: 81-52-744-2012; E-mail: storiha@med.nagoya-u.ac.jp.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Fujita, J. Cheng, K. Tauchi-Sato, T. Takenawa, and T. Fujimoto
A distinct pool of phosphatidylinositol 4,5-bisphosphate in caveolae revealed by a nanoscale labeling technique
PNAS, June 9, 2009; 106(23): 9256 - 9261.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. Abramowitz and L. Birnbaumer
Physiology and pathophysiology of canonical transient receptor potential channels
FASEB J, February 1, 2009; 23(2): 297 - 328.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. M. A. M. Kusters, W. P. M. van Meerwijk, D. L. Ypey, A. P. R. Theuvenet, and C. C. A. M. Gielen
Fast calcium wave propagation mediated by electrically conducted excitation and boosted by CICR
Am J Physiol Cell Physiol, April 1, 2008; 294(4): C917 - C930.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
R. J. Lang, H. Hashitani, M. A. Tonta, H. C. Parkington, and H. Suzuki
Spontaneous electrical and Ca2+ signals in typical and atypical smooth muscle cells and interstitial cell of Cajal-like cells of mouse renal pelvis
J. Physiol., September 15, 2007; 583(3): 1049 - 1068.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
Y. Zhu, J. Ye, and J. D. Huizinga
Clotrimazole-sensitive K+ currents regulate pacemaker activity in interstitial cells of Cajal
Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1715 - G1725.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. Sun, Y. Liu, S. Lipsky, and M. Cho
Physical manipulation of calcium oscillations facilitates osteodifferentiation of human mesenchymal stem cells
FASEB J, May 1, 2007; 21(7): 1472 - 1480.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Nakayama, K. Shimono, H.-N. Liu, H. Jiko, N. Katayama, T. Tomita, and K. Goto
Pacemaker phase shift in the absence of neural activity in guinea-pig stomach: a microelectrode array study
J. Physiol., November 1, 2006; 576(3): 727 - 738.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
C. V. Remillard and J. X.-J. Yuan
Transient Receptor Potential Channels and Caveolin-1: Good Friends in Tight Spaces
Mol. Pharmacol., October 1, 2006; 70(4): 1151 - 1154.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
E. Bradley, M. A. Hollywood, L. Johnston, R. J. Large, T. Matsuda, A. Baba, N. G. McHale, K. D. Thornbury, and G. P. Sergeant
Contribution of reverse Na+-Ca2+ exchange to spontaneous activity in interstitial cells of Cajal in the rabbit urethra
J. Physiol., August 1, 2006; 574(3): 651 - 661.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
M. Takaki, S. Nakayama, H. Misawa, T. Nakagawa, and H. Kuniyasu
In Vitro Formation of Enteric Neural Network Structure in a Gut-Like Organ Differentiated from Mouse Embryonic Stem Cells
Stem Cells, June 1, 2006; 24(6): 1414 - 1422.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
C. D. Hardin and J. Vallejo
Caveolins in vascular smooth muscle: Form organizing function
Cardiovasc Res, March 1, 2006; 69(4): 808 - 815.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
D. L. Cioffi, S. Wu, M. Alexeyev, S. R. Goodman, M. X. Zhu, and T. Stevens
Activation of the Endothelial Store-Operated ISOC Ca2+ Channel Requires Interaction of Protein 4.1 With TRPC4
Circ. Res., November 25, 2005; 97(11): 1164 - 1172.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Nakayama, S. Ohya, H.-N. Liu, T. Watanabe, S. Furuzono, J. Wang, Y. Nishizawa, M. Aoyama, N. Murase, T. Matsubara, et al.
Sulphonylurea receptors differently modulate ICC pacemaker Ca2+ activity and smooth muscle contractility
J. Cell Sci., September 15, 2005; 118(18): 4163 - 4173.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
E. Bradley, M. A. Hollywood, N. G. McHale, K. D. Thornbury, and G. P. Sergeant
Pacemaker activity in urethral interstitial cells is not dependent on capacitative calcium entry
Am J Physiol Cell Physiol, September 1, 2005; 289(3): C625 - C632.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. Y Estevez and K. Strange
Calcium feedback mechanisms regulate oscillatory activity of a TRP-like Ca2+ conductance in C. elegans intestinal cells
J. Physiol., August 15, 2005; 567(1): 239 - 251.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. S. Facemire and W. J. Arendshorst
Calmodulin mediates norepinephrine-induced receptor-operated calcium entry in preglomerular resistance arteries
Am J Physiol Renal Physiol, July 1, 2005; 289(1): F127 - F136.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. H. Ratz, K. M. Berg, N. H. Urban, and A. S. Miner
Regulation of smooth muscle calcium sensitivity: KCl as a calcium-sensitizing stimulus
Am J Physiol Cell Physiol, April 1, 2005; 288(4): C769 - C783.
[Abstract] [Full Text] [PDF]

Home page
 J Biol RhythmsHome page
H.-N. Liu, S. Ohya, S. Furuzono, J. Wang, Y. Imaizumi, and S. Nakayama
Co-contribution of IP3R and Ca{superscript 2}+ Influx Pathways to Pacemaker Ca{superscript 2}+ Activity in Stomach ICC
J Biol Rhythms, February 1, 2005; 20(1): 15 - 26.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. S. Murthy, H. Zhou, J. Huang, and S. N. Pentyala
Activation of PLC-{delta}1 by Gi/o-coupled receptor agonists
Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1679 - C1687.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. M. Wetzel, L. A. Chen, F. A. Ruiz, S. N. J. Moreno, and L. D. Sibley
Calcium-mediated protein secretion potentiates motility in Toxoplasma gondii
J. Cell Sci., November 15, 2004; 117(24): 5739 - 5748.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Aoyama, A. Yamada, J. Wang, S. Ohya, S. Furuzono, T. Goto, S. Hotta, Y. Ito, T. Matsubara, K. Shimokata, et al.
Requirement of ryanodine receptors for pacemaker Ca2+ activity in ICC and HEK293 cells
J. Cell Sci., June 1, 2004; 117(13): 2813 - 2825.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Ishikawa, S. Nakayama, T. Nakagawa, K. Horiguchi, H. Misawa, M. Kadowaki, A. Nakao, S. Inoue, T. Komuro, and M. Takaki
Characterization of in vitro gutlike organ formed from mouse embryonic stem cells
Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1344 - C1352.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Wang, M. Haas, M. Liang, T. Cai, J. Tian, S. Li, and Z. Xie
Ouabain Assembles Signaling Cascades through the Caveolar Na+/K+-ATPase
J. Biol. Chem., April 23, 2004; 279(17): 17250 - 17259.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
G. Boddy, A. Bong, W. Cho, and E. E. Daniel
ICC pacing mechanisms in intact mouse intestine differ from those in cultured or dissected intestine
Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G653 - G662.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. Dalrymple, D. M. Slater, L. Poston, and R. M. Tribe
Physiological Induction of Transient Receptor Potential Canonical Proteins, Calcium Entry Channels, in Human Myometrium: Influence of Pregnancy, Labor, and Interleukin-1{beta}
J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1291 - 1300.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Cayouette, M. P. Lussier, E.-L. Mathieu, S. M. Bousquet, and G. Boulay
Exocytotic Insertion of TRPC6 Channel into the Plasma Membrane upon Gq Protein-coupled Receptor Activation
J. Biol. Chem., February 20, 2004; 279(8): 7241 - 7246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
E. E. Daniel, G. Boddy, A. Bong, and W. Cho
A new model of pacing in the mouse intestine
Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G253 - G262.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Ordog, D. Redelman, N. N. Horowitz, and K. M. Sanders
Immunomagnetic enrichment of interstitial cells of Cajal
Am J Physiol Gastrointest Liver Physiol, February 1, 2004; 286(2): G351 - G360.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
M. Yamaga, M. Sekimata, M. Fujii, K. Kawai, H. Kamata, H. Hirata, Y. Homma, and H. Yagisawa
A PLC{delta}1-binding protein, p122/RhoGAP, is localized in caveolin-enriched membrane domains and regulates caveolin internalization
Genes Cells, January 1, 2004; 9(1): 25 - 37.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. Bergdahl, M. F. Gomez, K. Dreja, S.-Z. Xu, M. Adner, D. J. Beech, J. Broman, P. Hellstrand, and K. Sward
Cholesterol Depletion Impairs Vascular Reactivity to Endothelin-1 by Reducing Store-Operated Ca2+ Entry Dependent on TRPC1
Circ. Res., October 31, 2003; 93(9): 839 - 847.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
A. Y. Estevez, R. K. Roberts, and K. Strange
Identification of Store-independent and Store-operated Ca2+ Conductances in Caenorhabditis elegans Intestinal Epithelial Cells
J. Gen. Physiol., July 28, 2003; 122(2): 207 - 223.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-c. W. Brazer, B. B. Singh, X. Liu, W. Swaim, and I. S. Ambudkar
Caveolin-1 Contributes to Assembly of Store-operated Ca2+ Influx Channels by Regulating Plasma Membrane Localization of TRPC1
J. Biol. Chem., July 11, 2003; 278(29): 27208 - 27215.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. L. Walker, S. D. Koh, G. P. Sergeant, K. M. Sanders, and B. Horowitz
TRPC4 currents have properties similar to the pacemaker current in interstitial cells of Cajal
Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1637 - C1645.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement