| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 21, 19220-19228, May 24, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Transient expression of
constitutively active Rac1 derivatives, (G12V) or (Q61L), was
sufficient to induce phagocyte NADPH oxidase activity in a COS-7
cell model in which human cDNAs for essential oxidase components,
gp91phox, p22phox, p47phox, and
p67phox, were expressed as stable transgenes. Expression of
constitutively active Rac1 in "COSphox" cells induced
translocation of p47phox and p67phox to the membrane.
Furthermore, translocation of p47phox was induced in the
absence of p67phox expression, even though Rac does not
directly bind p47phox. Rac effector domain point substitutions
(A27K, G30S, D38A, Y40C), which can selectively eliminate interaction
with different effector proteins, impaired Rac1V12-induced superoxide
production. Activation of endogenous Rac1 by expression of
constitutively active Rac-guanine nucleotide exchange factor (GEF)
derivatives was sufficient to induce high level NADPH oxidase activity
in COSphox cells. The constitutively active form of the
hematopoietic-specific GEF, Vav1, was the most effective at
activating superoxide production, despite detection of higher levels of
Rac1-GTP upon expression of constitutively active Vav2 or Tiam1
derivatives. These data suggest that Rac can play a dual role in NADPH
oxidase activation, both by directly participating in the oxidase
complex and by activating signaling events leading to oxidase assembly,
and that Vav1 may be the physiologically relevant GEF responsible for
activating this Rac-regulated complex.
Rac Activation Induces NADPH Oxidase Activity in Transgenic
COSphox Cells, and the Level of Superoxide Production Is
Exchange Factor-dependent*
§¶,
,§¶
Herman B Wells Center for Pediatric
Research, § Department of Pediatrics (Hematology/Oncology),
James Whitcomb Riley Hospital for Children, the ¶ Department of
Medical and Molecular Genetics, and the Department of Medicine
(Nephrology), Indiana University Medical Center, Indianapolis, Indiana
46202 and the ** Department of Immunology, The Scripps
Research Institute, La Jolla, California 92037
*
This work was supported by National Institutes of Health
Grants RO1HL45635 (to M. C. D.), AI35947 (to U. G. K.), GM37696 (to U. G. K.) and by the Riley Memorial Association (to M. C. D.). The
facilities in The Indiana Center for Biological Microscopy were
supported in part by a grant from the Lilly Foundation (Indiana Genomics Initiative) to Indiana University School of Medicine and by an Indiana University Cancer Center Support Grant (NCI P30CA82709). The Wells Center for Pediatric Research is a Center for
Excellence in Molecular Hematology funded by P50DK49218.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Wells Center for
Pediatric Research, 1044 West Walnut St., R4, Rm. 402A, Indianapolis, IN 46202-5225. Tel.: 317-274-8645; Fax: 317-274-8679; E-mail: mdinauer@iupui.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Harmon and L. Ratner Induction of the G{alpha}q Signaling Cascade by the Human Immunodeficiency Virus Envelope Is Required for Virus Entry J. Virol., September 15, 2008; 82(18): 9191 - 9205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ugolev, Y. Berdichevsky, C. Weinbaum, and E. Pick Dissociation of Rac1(GDP){middle dot}RhoGDI Complexes by the Cooperative Action of Anionic Liposomes Containing Phosphatidylinositol 3,4,5-Trisphosphate, Rac Guanine Nucleotide Exchange Factor, and GTP J. Biol. Chem., August 8, 2008; 283(32): 22257 - 22271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Rygiel, A. E. Mertens, K. Strumane, R. van der Kammen, and J. G. Collard The Rac activator Tiam1 prevents keratinocyte apoptosis by controlling ROS-mediated ERK phosphorylation J. Cell Sci., April 15, 2008; 121(8): 1183 - 1192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Baumer, H. ten Freyhaus, H. Sauer, M. Wartenberg, K. Kappert, P. Schnabel, C. Konkol, J. Hescheler, M. Vantler, and S. Rosenkranz Phosphatidylinositol 3-Kinase-dependent Membrane Recruitment of Rac-1 and p47phox Is Critical for {alpha}-Platelet-derived Growth Factor Receptor-induced Production of Reactive Oxygen Species J. Biol. Chem., March 21, 2008; 283(12): 7864 - 7876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Roepstorff, I. Rasmussen, M. Sawada, C. Cudre-Maroux, P. Salmon, G. Bokoch, B. van Deurs, and F. Vilhardt Stimulus-dependent Regulation of the Phagocyte NADPH Oxidase by a VAV1, Rac1, and PAK1 Signaling Axis J. Biol. Chem., March 21, 2008; 283(12): 7983 - 7993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Choi, T. L. Leto, L. Hunyady, K. J. Catt, Y. S. Bae, and S. G. Rhee Mechanism of Angiotensin II-induced Superoxide Production in Cells Reconstituted with Angiotensin Type 1 Receptor and the Components of NADPH Oxidase J. Biol. Chem., January 4, 2008; 283(1): 255 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chen, R. He, R. D. Minshall, M. C. Dinauer, and R. D. Ye Characterization of a Mutation in the Phox Homology Domain of the NADPH Oxidase Component p40phox Identifies A Mechanism for Negative Regulation of Superoxide Production J. Biol. Chem., October 12, 2007; 282(41): 30273 - 30284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Fumagalli, H. Zhang, A. Baruzzi, C. A. Lowell, and G. Berton The Src Family Kinases Hck and Fgr Regulate Neutrophil Responses to N-Formyl-Methionyl-Leucyl-Phenylalanine J. Immunol., March 15, 2007; 178(6): 3874 - 3885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Ming, S. Li, D. D. Billadeau, L. A. Quilliam, and M. C. Dinauer The Rac Effector p67phox Regulates Phagocyte NADPH Oxidase by Stimulating Vav1 Guanine Nucleotide Exchange Activity Mol. Cell. Biol., January 1, 2007; 27(1): 312 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhu, C. C. Marchal, A.-J. Casbon, N. Stull, K. von Lohneysen, U. G. Knaus, A. J. Jesaitis, S. McCormick, W. M. Nauseef, and M. C. Dinauer Deletion Mutagenesis of p22phox Subunit of Flavocytochrome b558: IDENTIFICATION OF REGIONS CRITICAL FOR gp91phox MATURATION AND NADPH OXIDASE ACTIVITY J. Biol. Chem., October 13, 2006; 281(41): 30336 - 30346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Ellson, K. Davidson, G. J. Ferguson, R. O'Connor, L. R. Stephens, and P. T. Hawkins Neutrophils from p40phox-/- mice exhibit severe defects in NADPH oxidase regulation and oxidant-dependent bacterial killing J. Exp. Med., August 7, 2006; 203(8): 1927 - 1937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-I. Suh, N. D. Stull, X. J. Li, W. Tian, M. O. Price, S. Grinstein, M. B. Yaffe, S. Atkinson, and M. C. Dinauer The phosphoinositide-binding protein p40phox activates the NADPH oxidase during Fc{gamma}IIA receptor-induced phagocytosis J. Exp. Med., August 7, 2006; 203(8): 1915 - 1925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Wilkinson, J. Koenigsknecht-Talboo, C. Grommes, C. Y. D. Lee, and G. Landreth Fibrillar beta-Amyloid-stimulated Intracellular Signaling Cascades Require Vav for Induction of Respiratory Burst and Phagocytosis in Monocytes and Microglia J. Biol. Chem., July 28, 2006; 281(30): 20842 - 20850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ugolev, S. Molshanski-Mor, C. Weinbaum, and E. Pick Liposomes Comprising Anionic but Not Neutral Phospholipids Cause Dissociation of Rac(1 or 2){middle dot}RhoGDI Complexes and Support Amphiphile-independent NADPH Oxidase Activation by Such Complexes J. Biol. Chem., July 14, 2006; 281(28): 19204 - 19219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Cheng, B. A. Diebold, Y. Hughes, and J. D. Lambeth Nox1-dependent Reactive Oxygen Generation Is Regulated by Rac1 J. Biol. Chem., June 30, 2006; 281(26): 17718 - 17726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Hordijk Regulation of NADPH Oxidases: The Role of Rac Proteins Circ. Res., March 3, 2006; 98(4): 453 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kim and M. C. Dinauer Impaired NADPH oxidase activity in Rac2-deficient murine neutrophils does not result from defective translocation of p47phox and p67phox and can be rescued by exogenous arachidonic acid J. Leukoc. Biol., January 1, 2006; 79(1): 223 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wojnowski, B. Kulle, M. Schirmer, G. Schluter, A. Schmidt, A. Rosenberger, S. Vonhof, H. Bickeboller, M. R. Toliat, E.-K. Suk, et al. NAD(P)H Oxidase and Multidrug Resistance Protein Genetic Polymorphisms Are Associated With Doxorubicin-Induced Cardiotoxicity Circulation, December 13, 2005; 112(24): 3754 - 3762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Mollnau, M. Oelze, M. August, M. Wendt, A. Daiber, E. Schulz, S. Baldus, A. L. Kleschyov, A. Materne, P. Wenzel, et al. Mechanisms of Increased Vascular Superoxide Production in an Experimental Model of Idiopathic Dilated Cardiomyopathy Arterioscler. Thromb. Vasc. Biol., December 1, 2005; 25(12): 2554 - 2559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Martyn, M.-J. Kim, M. T. Quinn, M. C. Dinauer, and U. G. Knaus p21-activated kinase (Pak) regulates NADPH oxidase activation in human neutrophils Blood, December 1, 2005; 106(12): 3962 - 3969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Dudley Jr, N. E. Hoch, L. A. McCann, C. Honeycutt, L. Diamandopoulos, T. Fukai, D. G. Harrison, S. I. Dikalov, and J. Langberg Atrial Fibrillation Increases Production of Superoxide by the Left Atrium and Left Atrial Appendage: Role of the NADPH and Xanthine Oxidases Circulation, August 30, 2005; 112(9): 1266 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Cao, K. M. Mak, and C. S. Lieber Cytochrome P4502E1 primes macrophages to increase TNF-{alpha} production in response to lipopolysaccharide Am J Physiol Gastrointest Liver Physiol, July 1, 2005; 289(1): G95 - G107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. van Buul, E. C. Anthony, M. Fernandez-Borja, K. Burridge, and P. L. Hordijk Proline-rich Tyrosine Kinase 2 (Pyk2) Mediates Vascular Endothelial-Cadherin-based Cell-Cell Adhesion by Regulating {beta}-Catenin Tyrosine Phosphorylation J. Biol. Chem., June 3, 2005; 280(22): 21129 - 21136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Connolly, J. Rice, L. A. Feig, and R. J. Buchsbaum Tiam1-IRSp53 Complex Formation Directs Specificity of Rac-Mediated Actin Cytoskeleton Regulation Mol. Cell. Biol., June 1, 2005; 25(11): 4602 - 4614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kanekura, Y. Hashimoto, Y. Kita, J. Sasabe, S. Aiso, I. Nishimoto, and M. Matsuoka A Rac1/Phosphatidylinositol 3-Kinase/Akt3 Anti-apoptotic Pathway, Triggered by AlsinLF, the Product of the ALS2 Gene, Antagonizes Cu/Zn-superoxide Dismutase (SOD1) Mutant-induced Motoneuronal Cell Death J. Biol. Chem., February 11, 2005; 280(6): 4532 - 4543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N Fortemaison, F Miot, J E Dumont, and S Dremier Regulation of H2O2 generation in thyroid cells does not involve Rac1 activation Eur. J. Endocrinol., January 1, 2005; 152(1): 127 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Brandes and J. Kreuzer Vascular NADPH oxidases: molecular mechanisms of activation Cardiovasc Res, January 1, 2005; 65(1): 16 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Swanson and A. D. Hoppe The coordination of signaling during Fc receptor-mediated phagocytosis J. Leukoc. Biol., December 1, 2004; 76(6): 1093 - 1103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Quinn and K. A. Gauss Structure and regulation of the neutrophil respiratory burst oxidase: comparison with nonphagocyte oxidases J. Leukoc. Biol., October 1, 2004; 76(4): 760 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S. Park, S. H. Lee, D. Park, J. S. Lee, S. H. Ryu, W. J. Lee, S. G. Rhee, and Y. S. Bae Sequential Activation of Phosphatidylinositol 3-Kinase, {beta}Pix, Rac1, and Nox1 in Growth Factor-Induced Production of H2O2 Mol. Cell. Biol., May 15, 2004; 24(10): 4384 - 4394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-Y. Shin, K.-N. Woo, C.-S. Lee, S.-H. Koo, Y. G. Kim, W.-J. Kim, C.-D. Bae, S.-I. Chang, and E.-G. Kim Basic Fibroblast Growth Factor Stimulates Activation of Rac1 through a p85 {beta}PIX Phosphorylation-dependent Pathway J. Biol. Chem., January 16, 2004; 279(3): 1994 - 2004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Werner GTPases and reactive oxygen species: switches for killing and signaling J. Cell Sci., January 15, 2004; 117(2): 143 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kim, C. C. Marchal, J. Penninger, and M. C. Dinauer The Hemopoietic Rho/Rac Guanine Nucleotide Exchange Factor Vav1 Regulates N-Formyl-Methionyl-Leucyl-Phenylalanine-Activated Neutrophil Functions J. Immunol., October 15, 2003; 171(8): 4425 - 4430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Maack, T. Kartes, H. Kilter, H.-J. Schafers, G. Nickenig, M. Bohm, and U. Laufs Oxygen Free Radical Release in Human Failing Myocardium Is Associated With Increased Activity of Rac1-GTPase and Represents a Target for Statin Treatment Circulation, September 30, 2003; 108(13): 1567 - 1574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Wu, Y. Gu, Y. C. Xu, F. E. Nwariaku, and L. S. Terada Vascular Endothelial Growth Factor Causes Translocation of p47phox to Membrane Ruffles through WAVE1 J. Biol. Chem., September 19, 2003; 278(38): 36830 - 36840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sigal, Y. Gorzalczany, R. Sarfstein, C. Weinbaum, Y. Zheng, and E. Pick The Guanine Nucleotide Exchange Factor Trio Activates the Phagocyte NADPH Oxidase in the Absence of GDP to GTP Exchange on Rac. "THE EMPEROR'S NEW CLOTHES" J. Biol. Chem., February 7, 2003; 278(7): 4854 - 4861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Vilhardt, O. Plastre, M. Sawada, K. Suzuki, M. Wiznerowicz, E. Kiyokawa, D. Trono, and K.-H. Krause The HIV-1 Nef Protein and Phagocyte NADPH Oxidase Activation J. Biol. Chem., October 25, 2002; 277(44): 42136 - 42143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Bokoch and B. A. Diebold Current molecular models for NADPH oxidase regulation by Rac GTPase Blood, September 26, 2002; 100(8): 2692 - 2695. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
