JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M112377200 on March 20, 2002

J. Biol. Chem., Vol. 277, Issue 22, 19255-19264, May 31, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/22/19255    most recent
M112377200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kageyama, K.
Right arrow Articles by Kondo, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kageyama, K.
Right arrow Articles by Kondo, T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Overexpression of Calreticulin Modulates Protein Kinase B/Akt Signaling to Promote Apoptosis during Cardiac Differentiation of Cardiomyoblast H9c2 Cells*

Kan KageyamaDagger §, Yoshito IharaDagger ||, Shinji GotoDagger , Yoshishige UrataDagger , Genji Toda§, Katsusuke Yano§, and Takahito KondoDagger

From the Dagger  Department of Biochemistry and Molecular Biology in Disease, Atomic Bomb Disease Institute, and the § Third Department of Internal Medicine, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan

Calreticulin is a Ca2+-binding molecular chaperone of the lumen of the endoplasmic reticulum. Calreticulin has been shown to be essential for cardiac and neural development in mice, but the mechanism by which it functions in cell differentiation is not fully understood. To examine the role of calreticulin in cardiac differentiation, the calreticulin gene was introduced into rat cardiomyoblast H9c2 cells, and the effect of calreticulin overexpression on cardiac differentiation was examined. Upon culture in a differentiation medium containing fetal calf serum (1%) and retinoic acid (10 nM), cells transfected with the calreticulin gene were highly susceptible to apoptosis compared with controls. In the gene-transfected cells, protein kinase B/Akt signaling was significantly suppressed during differentiation. Furthermore, protein phosphatase 2A, a Ser/Thr protein phosphatase, was significantly up-regulated, implying suppression of Akt signaling due to dephosphorylation of Akt by the up-regulated protein phosphatase 2A via regulation of Ca2+ homeostasis. Thus, overexpression of calreticulin promotes differentiation-dependent apoptosis in H9c2 cells by suppressing the Akt signaling pathway. These findings indicate a novel mechanism by which cytoplasmic Akt signaling is modulated to cause apoptosis by a resident protein of the endoplasmic reticulum, calreticulin.

* This work was supported in part by grants-in-aid from the Ministry of Education, Science, Sports, and Culture of Japan and by the Japan Foundation of Cardiovascular Research.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Both authors contributed equally to this work.

|| To whom correspondence should be addressed. Tel.: 81-95-849-7099; Fax: 81-95-849-7100; E-mail: y-ihara@net.nagasaki-u.ac.jp.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Reproductive SciencesHome page
K. Meyer and Lubo Zhang
Fetal Programming of Cardiac Function and Disease
Reproductive Sciences, April 1, 2007; 14(3): 209 - 216.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. C. Sucharov, S. Langer, M. Bristow, and L. Leinwand
Shuttling of HDAC5 in H9C2 cells regulates YY1 function through CaMKIV/PKD and PP2A
Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1029 - C1037.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Hayashida, Y. Urata, E. Muroi, T. Kono, Y. Miyata, K. Nomata, H. Kanetake, T. Kondo, and Y. Ihara
Calreticulin Represses E-cadherin Gene Expression in Madin-Darby Canine Kidney Cells via Slug
J. Biol. Chem., October 27, 2006; 281(43): 32469 - 32484.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Kanda, Y. Ihara, H. Murata, Y. Urata, T. Kono, J. Yodoi, S. Seto, K. Yano, and T. Kondo
Glutaredoxin Modulates Platelet-derived Growth Factor-dependent Cell Signaling by Regulating the Redox Status of Low Molecular Weight Protein-tyrosine Phosphatase
J. Biol. Chem., September 29, 2006; 281(39): 28518 - 28528.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Okunaga, Y. Urata, S. Goto, T. Matsuo, S. Mizota, K. Tsutsumi, I. Nagata, T. Kondo, and Y. Ihara
Calreticulin, a Molecular Chaperone in the Endoplasmic Reticulum, Modulates Radiosensitivity of Human Glioblastoma U251MG Cells.
Cancer Res., September 1, 2006; 66(17): 8662 - 8671.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
Y. Wu, K. Zu, M. A. Warren, P. K. Wallace, and C. Ip
Delineating the mechanism by which selenium deactivates Akt in prostate cancer cells.
Mol. Cancer Ther., February 1, 2006; 5(2): 246 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Ihara, Y. Urata, S. Goto, and T. Kondo
Role of calreticulin in the sensitivity of myocardiac H9c2 cells to oxidative stress caused by hydrogen peroxide
Am J Physiol Cell Physiol, January 1, 2006; 290(1): C208 - C221.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Feriotto, A. Finotti, P. Volpe, S. Treves, S. Ferrari, C. Angelelli, F. Zorzato, and R. Gambari
Myocyte Enhancer Factor 2 Activates Promoter Sequences of the Human A{beta}H-J-J Locus, Encoding Aspartyl-{beta}-Hydroxylase, Junctin, and Junctate
Mol. Cell. Biol., April 15, 2005; 25(8): 3261 - 3275.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Yasuoka, Y. Ihara, S. Ikeda, Y. Miyahara, T. Kondo, and S. Kohno
Antiapoptotic Activity of Akt Is Down-regulated by Ca2+ in Myocardiac H9c2 Cells: EVIDENCE OF Ca2+-DEPENDENT REGULATION OF PROTEIN PHOSPHATASE 2Ac
J. Biol. Chem., December 3, 2004; 279(49): 51182 - 51192.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
H. A. Golpon, C. D. Coldren, M. R. Zamora, G. P. Cosgrove, M. D. Moore, R. M. Tuder, M. W. Geraci, and N. F. Voelkel
Emphysema Lung Tissue Gene Expression Profiling
Am. J. Respir. Cell Mol. Biol., December 1, 2004; 31(6): 595 - 600.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
T. Takizawa, C. Tatematsu, K. Watanabe, K. Kato, and Y. Nakanishi
Cleavage of Calnexin Caused by Apoptotic Stimuli: Implication for the Regulation of Apoptosis
J. Biochem., September 1, 2004; 136(3): 399 - 405.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Mesaeli and C. Phillipson
Impaired p53 Expression, Function, and Nuclear Localization in Calreticulin-deficient Cells
Mol. Biol. Cell, April 1, 2004; 15(4): 1862 - 1870.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Murata, Y. Ihara, H. Nakamura, J. Yodoi, K. Sumikawa, and T. Kondo
Glutaredoxin Exerts an Antiapoptotic Effect by Regulating the Redox State of Akt
J. Biol. Chem., December 12, 2003; 278(50): 50226 - 50233.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. R. Yellaturu, M. Bhanoori, I. Neeli, and G. N. Rao
N-Ethylmaleimide Inhibits Platelet-derived Growth Factor BB-stimulated Akt Phosphorylation via Activation of Protein Phosphatase 2A
J. Biol. Chem., October 11, 2002; 277(42): 40148 - 40155.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. F. Rocnik, E. van der Veer, H. Cao, R. A. Hegele, and J. G. Pickering
Functional Linkage between the Endoplasmic Reticulum Protein Hsp47 and Procollagen Expression in Human Vascular Smooth Muscle Cells
J. Biol. Chem., October 4, 2002; 277(41): 38571 - 38578.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.