Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M200933200 on March 7, 2002

J. Biol. Chem., Vol. 277, Issue 22, 19358-19366, May 31, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/22/19358    most recent
M200933200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cho, H.-J.
Right arrow Articles by You, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cho, H.-J.
Right arrow Articles by You, H. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Oncogenic H-Ras Enhances DNA Repair through the Ras/Phosphatidylinositol 3-Kinase/Rac1 Pathway in NIH3T3 Cells
EVIDENCE FOR ASSOCIATION WITH REACTIVE OXYGEN SPECIES*

Hyun-Ju ChoDagger §, Hye Gwang Jeong§, Jung-Sup Lee§||, Eun-Rhan Woo§, Jin-Won Hyun**, Myung-Hee Chung**, and Ho Jin YouDagger §Dagger Dagger

From the Dagger  Department of Pharmacology, School of Medicine, the § Research Center for Proteineous Materials, and the Departments of  Pharmacy and || Biological Sciences, Chosun University, 375 Seosuk-dong, Kwangju 501-759, Korea and the ** Department of Pharmacology, Seoul National University College of Medicine, 28 Yongon-dong, Seoul 110-799, Korea

This study investigated the role of oncogenic H-Ras in DNA repair capacity in NIH3T3 cells. Expression of dominant-positive H-Ras (V12-H-Ras) enhanced the host cell reactivation of luciferase activity from UV-irradiated and cisplatin-treated plasmids and also increased the unscheduled DNA synthesis following cisplatin or UV treatment of cells. This observed enhancement of DNA repair capacity was inhibited by transient transfection with dominant-negative H-Ras (N17-H-Ras) or Rac1 (N17-Rac1) plasmids. Moreover, stable transfection of dominant-positive Rac1 (V12-Rac1) further enhanced DNA repair capacity. Because reactive oxygen species (ROS) are known to be a downstream effector of oncogenic Ras, we examined the role of ROS in DNA repair capacity. We found that ROS production by V12-H-Ras expression was mediated by the Ras/phosphatidylinositol 3-kinase (PI3K)/Rac1/NADPH oxidase-dependent pathway and that pretreatment of V12-H-Ras-transformed cells with an antioxidant (N-acetylcysteine) and an NADPH oxidase inhibitor (diphenyleneiodonium) decreased DNA repair capacity. Similarly, treatment with PI3K inhibitors (wortmannin and LY294002) inhibited the ability of oncogenic H-Ras to enhance DNA repair capacity. Furthermore, inhibition of the Ras/PI3K/Rac1/NADPH oxidase pathway resulted in increased sensitivity to cisplatin and UV in V12-H-Ras-expressing NIH3T3 cells. Taken together, these results provide evidence that oncogenic H-Ras activates DNA repair capacity through the Ras/PI3K/Rac1/NADPH oxidase-dependent pathway and that increased ROS production via this signaling pathway is required for enhancement of the DNA repair capacity induced by oncogenic H-Ras.

* This work was supported by the Ministry of Science and Technology of Korea and the Korea Science and Engineering Foundation through the Research Center for Proteineous Materials.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Dagger To whom correspondence should be addressed. Tel.: 82-62-230-6337; Fax: 82-62-233-3720; E-mail: hjyou@mail.chosun.ac.kr.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. B. Friis, K. G. Vorum, and I. H. Lambert
Volume-sensitive NADPH oxidase activity and taurine efflux in NIH3T3 mouse fibroblasts
Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1552 - C1565.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. B. Kopnin, L. S. Agapova, B. P. Kopnin, and P. M. Chumakov
Repression of Sestrin Family Genes Contributes to Oncogenic Ras-Induced Reactive Oxygen Species Up-regulation and Genetic Instability
Cancer Res., May 15, 2007; 67(10): 4671 - 4678.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. B. Weidhaas, D. M. Eisenmann, J. M. Holub, and S. V. Nallur
A Conserved RAS/Mitogen-Activated Protein Kinase Pathway Regulates DNA Damage-Induced Cell Death Postirradiation in Radelegans
Cancer Res., November 1, 2006; 66(21): 10434 - 10438.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
Y. Zhao, L. Chaiswing, V. Bakthavatchalu, T. D. Oberley, and D. K. St. Clair
Ras mutation promotes p53 activation and apoptosis of skin keratinocytes
Carcinogenesis, August 1, 2006; 27(8): 1692 - 1698.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Ohta, M. Ohmichi, T. Hayasaka, S. Mabuchi, M. Saitoh, J. Kawagoe, K. Takahashi, H. Igarashi, B. Du, M. Doshida, et al.
Inhibition of Phosphatidylinositol 3-Kinase Increases Efficacy of Cisplatin in in Vivo Ovarian Cancer Models
Endocrinology, April 1, 2006; 147(4): 1761 - 1769.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
I.-Y. Chang, C.-K. Youn, H.-B. Kim, M.-H. Kim, H.-J. Cho, Y. Yoon, Y.-S. Lee, M.-H. Chung, and H. J. You
Oncogenic H-Ras Up-regulates Expression of Ku80 to Protect Cells from {gamma}-Ray Irradiation in NIH3T3 Cells
Cancer Res., August 1, 2005; 65(15): 6811 - 6819.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
B. H. Jeon, G. Gupta, Y. C. Park, B. Qi, A. Haile, F. A. Khanday, Y.-X. Liu, J.-M. Kim, M. Ozaki, A. R. White, et al.
Apurinic/Apyrmidinic Endonuclease 1 Regulates Endothelial NO Production and Vascular Tone
Circ. Res., October 29, 2004; 95(9): 902 - 910.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C.-K. Youn, M.-H. Kim, H.-J. Cho, H.-B. Kim, I.-Y. Chang, M.-H. Chung, and H. J. You
Oncogenic H-Ras Up-Regulates Expression of ERCC1 to Protect Cells from Platinum-Based Anticancer Agents
Cancer Res., July 15, 2004; 64(14): 4849 - 4857.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
K.-H. Shin, M. K. Kang, E. Dicterow, A. Kameta, M. A. Baluda, and N.-H. Park
Introduction of Human Telomerase Reverse Transcriptase to Normal Human Fibroblasts Enhances DNA Repair Capacity
Clin. Cancer Res., April 1, 2004; 10(7): 2551 - 2560.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M.-R. Lee, S.-H. Kim, H.-J. Cho, K.-Y. Lee, A. R. Moon, H. G. Jeong, J.-S. Lee, J.-W. Hyun, M.-H. Chung, and H. J. You
Transcription Factors NF-YA Regulate the Induction of Human OGG1 Following DNA-alkylating Agent Methylmethane Sulfonate (MMS) Treatment
J. Biol. Chem., March 12, 2004; 279(11): 9857 - 9866.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement