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J. Biol. Chem., Vol. 277, Issue 22, 19665-19672, May 31, 2002
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From the Department of Nutrition, School of Medicine, Tokushima
University, Kuramoto-Cho 3, Tokushima City 770-8503, Japan
Growth is critically dependent on the retention
of a variety of nutrients. The kidney contributes to this positive
external balance. In the present study, we isolated a cDNA from the
human and rat kidney that encodes a growth-related
Na+-dependent inorganic phosphate
(Pi) cotransporter (type IIc). Microinjection of type IIc
cRNA into Xenopus oocytes demonstrated sodium-dependent Pi cotransport activity.
Affinity for Pi was 0.07 mM in 100 mM Na+. The transport activity was dependent on
extracellular pH. In electrophysiological studies, type IIc
Na/Pi cotransport was electroneutral, whereas type IIa was
highly electrogenic. In Northern blotting analysis, the type IIc
transcript was only expressed in the kidney and highly in weaning
animals. In immunohistochemical analysis, the type IIc protein was
shown to be localized at the apical membrane of the proximal tubular
cells in superficial and midcortical nephrons of weaning rat kidney.
Hybrid depletion experiments suggested that type IIc could function as
a Na/Pi cotransporter in weaning animals, but its role is
reduced in adults. The finding of the present study suggest that the
type IIc is a growth-related renal Na/Pi cotransporter,
which has a high affinity for Pi and is electroneutral.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AB055000 and AB077042.
Growth-related Renal Type II Na/Pi Cotransporter*
*
This work was supported by Grant 11557202 (to K. M.) from
the Ministry of Education, Science, Sports and Culture of Japan.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Nutrition,
School of Medicine, Tokushima University, Kuramoto-Cho 3, Tokushima
City 770-8503, Japan. Tel.: 81-886-7081; Fax: 81-886-33-7082; E-mail:
miyamoto@nutr.med.tokushima-u.ac.jp.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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