JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M201089200 on March 26, 2002

J. Biol. Chem., Vol. 277, Issue 23, 20379-20385, June 7, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/23/20379    most recent
M201089200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chiba, H.
Right arrow Articles by Voelker, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chiba, H.
Right arrow Articles by Voelker, D. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Human Surfactant Protein D (SP-D) Binds Mycoplasma pneumoniae by High Affinity Interactions with Lipids*

Hirofumi Chiba, Surapon Pattanajitvilai, Amanda J. Evans, Ronald J. Harbeck, and Dennis R. VoelkerDagger

From the Program in Cell Biology, Department of Medicine, National Jewish Medical and Research Center, Denver, Colorado 80206

Increasing evidence now identifies surfactant protein D (SP-D) as an important element of the innate immune system of the lung. In this study, we examined the interactions of rat and human SP-D with the human pathogen, Mycoplasma pneumoniae. Rat and human SP-D bound the organism with high affinity in a reaction that required Ca2+ and was inhibited by EGTA. Membranes derived from the organism bound the proteins in a similar manner, except the rat SP-D also exhibited a significant level of Ca2+-independent binding. Pretreatment of membranes with proteases did not alter the Ca2+-dependent SP-D binding of membranes by either protein. Mannose, glucose, maltose, and inositol, at millimolar concentrations, competed for human SP-D binding to the bacterial membrane. Lipids extracted from membranes and separated by two-dimensional thin layer chromatography bound human SP-D with high affinity in a Ca2+-dependent reaction. A tandem mutant of SP-D with E321Q and N323D substitutions, failed to bind M. pneumoniae lipids, directly implicating the carbohydrate recognition domain in the interaction. The interaction of rat and human SP-D with M. pneumoniae was unaffected by the presence of surfactant lipids and the hydrophobic surfactant proteins. These findings demonstrate that M. pneumoniae is likely to be recognized by SP-D in the alveolar environment and that primary determinants recognized on the organism are lipid components of the cell membrane.

* This work was supported by National Institutes of Health Grants HL 45286 and ALA-ARC 95.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Medicine, National Jewish Medical Research Center, 1400 Jackson St., Denver, CO 80206. Tel.: 303-398-1300; Fax: 303-398-1806; E-mail: voelkerd@njc.org.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. Chmura, X. Bai, M. Nakamura, P. Kandasamy, M. McGibney, K. Kuronuma, H. Mitsuzawa, D. R. Voelker, and E. D. Chan
Induction of IL-8 by Mycoplasma pneumoniae membrane in BEAS-2B cells
Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L220 - L230.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
H. Sahly, Y. Keisari, E. Crouch, N. Sharon, and I. Ofek
Recognition of Bacterial Surface Polysaccharides by Lectins of the Innate Immune System and Its Contribution to Defense against Infection: the Case of Pulmonary Pathogens
Infect. Immun., April 1, 2008; 76(4): 1322 - 1332.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. Zhang, M. Ikegami, T. R. Korfhagen, F. X. McCormack, M. Yoshida, R. M. Senior, J. M. Shipley, S. D. Shapiro, and J. A. Whitsett
Neither SP-A nor NH2-terminal domains of SP-A can substitute for SP-D in regulation of alveolar homeostasis
Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L181 - L190.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. W. Chu, S. Jeyaseelan, J. G. Rino, D. R. Voelker, R. B. Wexler, K. Campbell, R. J. Harbeck, and R. J. Martin
TLR2 Signaling Is Critical for Mycoplasma pneumoniae-Induced Airway Mucin Expression
J. Immunol., May 1, 2005; 174(9): 5713 - 5719.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Piboonpocanun, H. Chiba, H. Mitsuzawa, W. Martin, R. C. Murphy, R. J. Harbeck, and D. R. Voelker
Surfactant Protein A Binds Mycoplasma pneumoniae with High Affinity and Attenuates Its Growth by Recognition of Disaturated Phosphatidylglycerols
J. Biol. Chem., January 7, 2005; 280(1): 9 - 17.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. X. McCormack, R. Gibbons, S. R. Ward, A. Kuzmenko, H. Wu, and G. S. Deepe Jr.
Macrophage-independent Fungicidal Action of the Pulmonary Collectins
J. Biol. Chem., September 19, 2003; 278(38): 36250 - 36256.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Nadesalingam, A. L. Bernal, A. W. Dodds, A. C. Willis, D. J. Mahoney, A. J. Day, K. B. M. Reid, and N. Palaniyar
Identification and Characterization of a Novel Interaction between Pulmonary Surfactant Protein D and Decorin
J. Biol. Chem., July 3, 2003; 278(28): 25678 - 25687.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. Rottem
Interaction of Mycoplasmas With Host Cells
Physiol Rev, April 1, 2003; 83(2): 417 - 432.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.