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J. Biol. Chem., Vol. 277, Issue 24, 21691-21696, June 14, 2002
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From the VlsE is an outer surface lipoprotein of
Borrelia burgdorferi that undergoes antigenic variation
through an elaborate gene conversion mechanism and is thought to play a
major role in the immune response to the Lyme disease borellia. The
crystal structure of recombinant variant protein VlsE1 at 2.3-Å
resolution reveals that the six variable regions form loop structures
that constitute most of the membrane distal surface of VlsE, covering
the predominantly The atomic coordinates and the structure factors (code 1L8W) have been deposited in the Protein Data Bank, Research Collaboratory for Structural Bioinformatics, Rutgers University, New Brunswick, NJ (http://www.rcsb.org/).
Crystal Structure of Lyme Disease Variable Surface Antigen
VlsE of Borrelia burgdorferi*
,,§,
,**
Center for Structural Biology, Texas A&M
University, College Station, Texas 77843-2128 and Albert B. Alkek
Institute of Biosciences and Technology, Houston, Texas 77030-3303 and
the ¶ Departments of Pathology and Laboratory Medicine and
Microbiology and Molecular Genetics, University of Texas Medical
School at Houston, Houston, Texas 77225
-helical, invariant regions of the protein.
The surface localization of the variable amino acid segments
appears to protect the conserved regions from interaction with
antibodies and hence may contribute to immune evasion.
*
This work was supported by the National Institutes of
Health, the Texas Advanced Technology Program, and the Welch
Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Pfizer, Inc., Animal Health Biological
Discovery, Eastern Point Rd., Groton, CT 06340.
**
To whom correspondence should be addressed. Tel.: 979-862-7636;
Fax: 979-862-7638; E-mail: sacchett@tamu.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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