HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 25, 22119-22122, June 21, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/25/22119    most recent C200215200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Greenwood, I. A. Articles by Horowitz, B. Search for Related Content PubMed PubMed Citation Articles by Greenwood, I. A. Articles by Horowitz, B.

ACCELERATED PUBLICATION
The Large Conductance Potassium Channel -Subunit Can Interact with and Modulate the Functional Properties of a Calcium-activated Chloride Channel, CLCA1^*

Iain A. Greenwood, Lisa J. Miller, Susumu Ohya, and Burton Horowitz^§

From the Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada 89557-0046

We have recently compared the biophysical and pharmacological properties of native Ca²⁺-activated Cl currents in murine portal vein with mCLCA1 channels cloned from murine portal vein myocytes (Britton, F. C., Ohya, S., Horowitz, B., and Greenwood, I. A. (2002) J. Physiol. (Lond.) 539, 107-117). These channels shared a similar relative permeability to various anions, but the expressed channel current lacked the marked time dependence of the native current. In addition, the expressed channel showed a lower Ca²⁺ sensitivity than the native channel. As non-pore-forming regulatory -subunits alter the kinetics and increase the Ca²⁺ sensitivity of Ca²⁺-dependent K⁺ channels (BK channels) we investigated whether co-expression of -subunits with CLCA1 would alter the kinetics/Ca²⁺ sensitivity of mCLCA1. Internal dialysis of human embryonic kidney cells stably expressing CLCA1 with 500 nM Ca²⁺ evoked a significantly larger current when the -subunit KCNMB1 was co-expressed. In a small number of co-transfected cells marked time dependence to the activation kinetics was observed. Interaction studies using the mammalian two-hybrid technique demonstrated a physical association between CLCA1 and KCNMB1 when co-expressed in human embryonic kidney cells. These data suggest that activation of CLCA1 can be modified by accessory subunits.

This article has been cited by other articles:

				M. K. Bothe, J. Braun, L. Mundhenk, and A. D. Gruber Murine mCLCA6 Is an Integral Apical Membrane Protein of Non-goblet Cell Enterocytes and Co-localizes With the Cystic Fibrosis Transmembrane Conductance Regulator J. Histochem. Cytochem., May 1, 2008; 56(5): 495 - 509. [Abstract] [Full Text] [PDF]

				L. H. Clapp and N. N. Orie Stoking Up BKCa Channels in Hemorrhagic Shock: Which Channel Subunit Is Really Fueling the Fire? Circ. Res., August 31, 2007; 101(5): 436 - 438. [Full Text] [PDF]

				S. N. Saleh, J. E. Angermann, W. R. Sones, N. Leblanc, and I. A. Greenwood Stimulation of Ca2+-Gated Cl- Currents by the Calcium-Dependent K+ Channel Modulators NS1619 [1,3-Dihydro-1-[2-hydroxy-5-(trifluoromethyl)phenyl]-5-(trifluoromethyl)-2H-benzimidazol-2-one] and Isopimaric Acid J. Pharmacol. Exp. Ther., June 1, 2007; 321(3): 1075 - 1084. [Abstract] [Full Text] [PDF]

				L. Mundhenk, M. Alfalah, R. C. Elble, B. U. Pauli, H. Y. Naim, and A. D. Gruber Both Cleavage Products of the mCLCA3 Protein Are Secreted Soluble Proteins J. Biol. Chem., October 6, 2006; 281(40): 30072 - 30080. [Abstract] [Full Text] [PDF]

				A. Gibson, A. P. Lewis, K. Affleck, A. J. Aitken, E. Meldrum, and N. Thompson hCLCA1 and mCLCA3 Are Secreted Non-integral Membrane Proteins and Therefore Are Not Ion Channels J. Biol. Chem., July 22, 2005; 280(29): 27205 - 27212. [Abstract] [Full Text] [PDF]

				M. E. Loewen and G. W. Forsyth Structure and Function of CLCA Proteins Physiol Rev, July 1, 2005; 85(3): 1061 - 1092. [Abstract] [Full Text] [PDF]

				S. R. Evans, W. B. Thoreson, and C. L. Beck Molecular and Functional Analyses of Two New Calcium-activated Chloride Channel Family Members from Mouse Eye and Intestine J. Biol. Chem., October 1, 2004; 279(40): 41792 - 41800. [Abstract] [Full Text] [PDF]

				M. E. Loewen, L. K. Bekar, W. Walz, G. W. Forsyth, and S. E. Gabriel pCLCA1 lacks inherent chloride channel activity in an epithelial colon carcinoma cell line Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G33 - G41. [Abstract] [Full Text] [PDF]

				J. Eggermont Calcium-activated Chloride Channels: (Un)known, (Un)loved? Proceedings of the ATS, January 1, 2004; 1(1): 22 - 27. [Abstract] [Full Text] [PDF]

				S. M. Foord Matching Accessories Sci. Signal., July 8, 2003; 2003(190): pe25 - pe25. [Abstract] [Full Text] [PDF]

				S. Ohya, G. P. Sergeant, I. A. Greenwood, and B. Horowitz Molecular Variants of KCNQ Channels Expressed in Murine Portal Vein Myocytes: A Role in Delayed Rectifier Current Circ. Res., May 16, 2003; 92(9): 1016 - 1023. [Abstract] [Full Text] [PDF]