JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M201895200 on April 15, 2002

J. Biol. Chem., Vol. 277, Issue 25, 22215-22221, June 21, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/25/22215    most recent
M201895200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brès, V.
Right arrow Articles by Benkirane, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brès, V.
Right arrow Articles by Benkirane, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Tat Acetyl-acceptor Lysines Are Important for Human Immunodeficiency Virus Type-1 Replication*

Vanessa BrèsDagger , Rosemary Kiernan§, Stéphane Emiliani, and Monsef Benkirane||

From the Institut de Génétique Humaine, CNRS UPR 1142, 141 rue de la Cardonille, 34396 Montpellier cedex 5, France

The human immunodeficiency virus type-1 trans-activator Tat is a transcription factor that activates the HIV-1 promoter through binding to the trans-activation-responsive region (TAR) localized at the 5'-end of all viral transcripts. We and others have recently shown that Tat is directly acetylated at lysine 28, within the activation domain, and lysine 50, in the TAR RNA binding domain, by Tat-associated histone acetyltransferases p300, p300/CBP-associating factor, and hGCN5. Here, we show that mutation of acetyl-acceptor lysines to arginine or glutamine affects virus replication. Interestingly, mutation of lysine 28 and lysine 50 differentially affected Tat trans-activation of integrated versus nonintegrated long terminal repeat. Our results highlight the importance of lysine 28 and lysine 50 of Tat in virus replication and Tat-mediated trans-activation.

* This work was supported by grants from the Agence Nationale de Recherche sur le SIDA (ANRS) and Action Concerte Initiative blanche (to M. B.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Supported by a Ministere de l'Education nationale, de la Recherche et de la Technologie scholarship.

§ Supported by an ANRS fellowship.

Present address: Institut Cochin de Génétique Moléculaire, INSERM U529, 24 rue du Faubourg Saint Jacques, 75014 Paris, France.

|| To whom correspondence should be addressed. Tel.: 33-4-99-61-99-32; Fax: 33-4-99-61-99-01; E-mail: bmonsef@igh.cnrs.fr.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Sharma, S. Awasthi, C. K. Harrod, E. F. Matlock, S. Khan, L. Xu, S. Chan, H. Yang, C. K. Thammavaram, R. A. Rasor, et al.
The Werner Syndrome Helicase Is a Cofactor for HIV-1 Long Terminal Repeat Transactivation and Retroviral Replication
J. Biol. Chem., April 20, 2007; 282(16): 12048 - 12057.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. Berro, K. Kehn, C. de la Fuente, A. Pumfery, R. Adair, J. Wade, A. M. Colberg-Poley, J. Hiscott, and F. Kashanchi
Acetylated Tat Regulates Human Immunodeficiency Virus Type 1 Splicing through Its Interaction with the Splicing Regulator p32.
J. Virol., April 1, 2006; 80(7): 3189 - 3204.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Ranjbar, R. Rajsbaum, and A. E. Goldfeld
Transactivator of Transcription from HIV Type 1 Subtype E Selectively Inhibits TNF Gene Expression via Interference with Chromatin Remodeling of the TNF Locus
J. Immunol., April 1, 2006; 176(7): 4182 - 4190.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Y. Desfosses, M. Solis, Q. Sun, N. Grandvaux, C. Van Lint, A. Burny, A. Gatignol, M. A. Wainberg, R. Lin, and J. Hiscott
Regulation of Human Immunodeficiency Virus Type 1 Gene Expression by Clade-Specific Tat Proteins
J. Virol., July 15, 2005; 79(14): 9180 - 9191.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
B. Xie, V. Calabro, M. A. Wainberg, and A. D. Frankel
Selection of TAR RNA-Binding Chameleon Peptides by Using a Retroviral Replication System
J. Virol., February 1, 2004; 78(3): 1456 - 1463.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Apolloni, C. W. Hooker, J. Mak, and D. Harrich
Human Immunodeficiency Virus Type 1 Protease Regulation of Tat Activity Is Essential for Efficient Reverse Transcription and Replication
J. Virol., September 15, 2003; 77(18): 9912 - 9921.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.