JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M202163200 on April 4, 2002

J. Biol. Chem., Vol. 277, Issue 25, 22297-22303, June 21, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/25/22297    most recent
M202163200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boner, W.
Right arrow Articles by Morgan, I. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boner, W.
Right arrow Articles by Morgan, I. M.

A Functional Interaction between the Human Papillomavirus 16 Transcription/Replication Factor E2 and the DNA Damage Response Protein TopBP1*

Winifred BonerDagger §, Ewan R. TaylorDagger , Emmanouella TsirimonakiDagger , Kazuhiko Yamane||**, M. Saveria CampoDagger , and Iain M. MorganDagger Dagger Dagger

From the Dagger  Institute of Comparative Medicine, Department of Veterinary Pathology, University of Glasgow, Garscube Estate, Bearsden Road, Glasgow G61 1QH, Scotland, the § Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1BD, Scotland, and the || Laboratory of Biomedical Research, Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113 0032, Japan

The human papillomavirus (HPV) transcription/replication factor E2 is essential for the life cycle of HPVs. E2 protein binds to DNA target sequences in the viral long control regions to regulate transcription of the viral genome. It also enhances viral DNA replication by interacting with the viral replication factor E1 and recruiting it to the origin of replication and may also play a more direct role in replication. The cellular proteins with which E2 interacts to carry out these functions are largely unknown. To identify these proteins a yeast two-hybrid screen was carried out with the transcription/replication domain of HPV16 E2. This screen identified several candidate interacting partners for E2 including TopBP1 (topoisomerase IIbeta -binding protein 1). TopBP1 has eight BRCA1 carboxyl-terminal domains that are found in proteins regulating the DNA damage response, transcription, and replication. Here we demonstrate that HPV16 E2 and TopBP1 interact in vitro and in vivo and that TopBP1 can enhance the ability of E2 to activate transcription and replication. This is the first time that TopBP1 has been shown to function as a transcriptional coactivator and that E2 interacts with TopBP1. Removal of the amino-terminal domain of TopBP1 abolishes coactivation of transcription and replication. This interaction may have functional consequences upon the viral life cycle.

* This work was supported in part by grants from the Association for International Cancer Research and the Scottish Hospital Endowment Research Trust.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of a Biotechnology and Biological Sciences Research Council Industrial Cooperative Award in Science and Engineering studentship.

** Present address: Radiation Oncology, Case Western Reserve University, Cleveland, OH 44106.

Dagger Dagger To whom correspondence should be addressed. Tel.: 44-141-330-3155; Fax: 44-141-330-5602; E-mail: i.morgan@vet.gla.ac.uk.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
E. Sjottem, C. Rekdal, G. Svineng, S. S. Johnsen, H. Klenow, R. D. Uglehus, and T. Johansen
The ePHD protein SPBP interacts with TopBP1 and together they co-operate to stimulate Ets1-mediated transcription
Nucleic Acids Res., October 8, 2007; 35(19): 6648 - 6662.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. M. Donaldson, W. Boner, and I. M. Morgan
TopBP1 Regulates Human Papillomavirus Type 16 E2 Interaction with Chromatin
J. Virol., April 15, 2007; 81(8): 4338 - 4342.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. Ajay Kumar, S. R. Naidu, X. Wang, A. N. Imbalzano, and E. J. Androphy
Interaction of Papillomavirus E2 Protein with the Brm Chromatin Remodeling Complex Leads to Enhanced Transcriptional Activation
J. Virol., March 1, 2007; 81(5): 2213 - 2220.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. R. Taylor, E. S. Dornan, W. Boner, J. A. Connolly, S. McNair, P. Kannouche, A. R. Lehmann, and I. M. Morgan
The Fidelity of HPV16 E1/E2-mediated DNA Replication
J. Biol. Chem., December 26, 2003; 278(52): 52223 - 52230.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Y. Hou, S.-Y. Wu, and C.-M. Chiang
Transcriptional Activity among High and Low Risk Human Papillomavirus E2 Proteins Correlates with E2 DNA Binding
J. Biol. Chem., November 15, 2002; 277(47): 45619 - 45629.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.