|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 27, 24120-24127, July 5, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Biochemistry and Molecular Biology,
Center for Mammalian Genetics, and Center for Nutritional Sciences,
University of Florida College of Medicine, Gainesville, Florida
32610-0245
Transcription from the asparagine synthetase
(A.S.) gene is increased in response to either amino acid
(amino acid response) or glucose (endoplasmic reticulum stress
response) deprivation. These two independent pathways converge on the
same set of genomic cis-elements within the
A.S. promoter referred to as nutrient-sensing response
elements (NSRE) 1 and 2, both of which are necessary for gene
activation. The NSRE-1 sequence was used to screen ATF/CREB family
members by electrophoresis mobility shift assays and supershift by specific antibodies. The results indicated that ATF4 binds to the
NSRE-1 sequence and that the amount of the ATF4 complex was increased
when extracts from amino acid-deprived or glucose-deprived cells were
tested. Using electrophoresis mobility shift assay experiments and a
probe that contained both NSRE-1 and NSRE-2, mutation of the NSRE-1
sequence completely prevented formation of the ATF4-containing
complexes, whereas mutation of the NSRE-2 sequence did not.
Overexpression of ATF4 increased A.S. promoter-driven transcription, whereas an inhibitory dominant negative ATF4 mutant blocked both basal and starvation-enhanced transcription. Collectively, the results provide both in vitro and in vivo
evidence for a role of ATF4 in the transcriptional activation of the
A.S. gene in response to nutrient deprivation.
ATF4 Is a Mediator of the Nutrient-sensing Response Pathway That
Activates the Human Asparagine Synthetase Gene*
*
This research was supported by Grant DK-52064 (to
M. S. K.) from the NIDDK, the National Institutes of Health.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Biochemistry
and Molecular Biology, University of Florida College of Medicine, Box
100245, Gainesville, FL 32610-0245. Tel.: 352-392-2711; Fax:
352-392-6511; E-mail: mkilberg@ufl.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Su and M. S. Kilberg C/EBP Homology Protein (CHOP) Interacts with Activating Transcription Factor 4 (ATF4) and Negatively Regulates the Stress-dependent Induction of the Asparagine Synthetase Gene J. Biol. Chem., December 12, 2008; 283(50): 35106 - 35117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gjymishka, S. S. Palii, J. Shan, and M. S. Kilberg Despite Increased ATF4 Binding at the C/EBP-ATF Composite Site following Activation of the Unfolded Protein Response, System A Transporter 2 (SNAT2) Transcription Activity Is Repressed in HepG2 Cells J. Biol. Chem., October 10, 2008; 283(41): 27736 - 27747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Fioravante, R.-Y. Liu, and J. H. Byrne The Ubiquitin-Proteasome System Is Necessary for Long-Term Synaptic Depression in Aplysia J. Neurosci., October 8, 2008; 28(41): 10245 - 10256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Thiaville, E. E. Dudenhausen, K. S. Awad, A. Gjymishka, C. Zhong, and M. S. Kilberg Activated transcription via mammalian amino acid response elements does not require enhanced recruitment of the Mediator complex Nucleic Acids Res., October 1, 2008; 36(17): 5571 - 5580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, E. Bevilacqua, C.-B. Chiribau, M. Majumder, C. Wang, C. M. Croniger, M. D. Snider, P. F. Johnson, and M. Hatzoglou Differential Control of the CCAAT/Enhancer-binding Protein {beta} (C/EBP{beta}) Products Liver-enriched Transcriptional Activating Protein (LAP) and Liver-enriched Transcriptional Inhibitory Protein (LIP) and the Regulation of Gene Expression during the Response to Endoplasmic Reticulum Stress J. Biol. Chem., August 15, 2008; 283(33): 22443 - 22456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. V. Oskolkova, T. Afonyushkin, A. Leitner, E. von Schlieffen, P. S. Gargalovic, A. J. Lusis, B. R. Binder, and V. N. Bochkov ATF4-dependent transcription is a key mechanism in VEGF up-regulation by oxidized phospholipids: critical role of oxidized sn-2 residues in activation of unfolded protein response Blood, July 15, 2008; 112(2): 330 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Malmberg and C. M. Adams Insulin Signaling and the General Amino Acid Control Response: TWO DISTINCT PATHWAYS TO AMINO ACID SYNTHESIS AND UPTAKE J. Biol. Chem., July 11, 2008; 283(28): 19229 - 19234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Lange, J. C. Chavez, J. T. Pinto, G. Coppola, C.-W. Sun, T. M. Townes, D. H. Geschwind, and R. R. Ratan ATF4 is an oxidative stress-inducible, prodeath transcription factor in neurons in vitro and in vivo J. Exp. Med., May 12, 2008; 205(5): 1227 - 1242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Thiaville, Y.-X. Pan, A. Gjymishka, C. Zhong, R. J. Kaufman, and M. S. Kilberg MEK Signaling Is Required for Phosphorylation of eIF2{alpha} following Amino Acid Limitation of HepG2 Human Hepatoma Cells J. Biol. Chem., April 18, 2008; 283(16): 10848 - 10857. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-I. Lee, J. E. Dominy Jr., A. K. Sikalidis, L. L. Hirschberger, W. Wang, and M. H. Stipanuk HepG2/C3A cells respond to cysteine deprivation by induction of the amino acid deprivation/integrated stress response pathway Physiol Genomics, April 1, 2008; 33(2): 218 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Watatani, K. Ichikawa, N. Nakanishi, M. Fujimoto, H. Takeda, N. Kimura, H. Hirose, S. Takahashi, and Y. Takahashi Stress-induced Translation of ATF5 mRNA Is Regulated by the 5'-Untranslated Region J. Biol. Chem., February 1, 2008; 283(5): 2543 - 2553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Cherasse, A.-C. Maurin, C. Chaveroux, C. Jousse, V. Carraro, L. Parry, C. Deval, C. Chambon, P. Fafournoux, and A. Bruhat The p300/CBP-associated factor (PCAF) is a cofactor of ATF4 for amino acid-regulated transcription of CHOP Nucleic Acids Res., September 27, 2007; 35(17): 5954 - 5965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Adams Role of the Transcription Factor ATF4 in the Anabolic Actions of Insulin and the Anti-anabolic Actions of Glucocorticoids J. Biol. Chem., June 8, 2007; 282(23): 16744 - 16753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jousse, C. Deval, A.-C. Maurin, L. Parry, Y. Cherasse, C. Chaveroux, R. Lefloch, P. Lenormand, A. Bruhat, and P. Fafournoux TRB3 Inhibits the Transcriptional Activation of Stress-regulated Genes by a Negative Feedback on the ATF4 Pathway J. Biol. Chem., May 25, 2007; 282(21): 15851 - 15861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Cui, S. Darmanin, M. Natsuisaka, T. Kondo, M. Asaka, M. Shindoh, F. Higashino, J. Hamuro, F. Okada, M. Kobayashi, et al. Enhanced Expression of Asparagine Synthetase under Glucose-Deprived Conditions Protects Pancreatic Cancer Cells from Apoptosis Induced by Glucose Deprivation and Cisplatin Cancer Res., April 1, 2007; 67(7): 3345 - 3355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Chen and M. S. Kilberg Alignment of the Transcription Start Site Coincides with Increased Transcriptional Activity from the Human Asparagine Synthetase Gene Following Amino Acid Deprivation of HepG2 Cells J. Nutr., October 1, 2006; 136(10): 2463 - 2467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Marchand, C. Tomkiewicz, L. Magne, R. Barouki, and M. Garlatti Endoplasmic Reticulum Stress Induction of Insulin-like Growth Factor-binding Protein-1 Involves ATF4 J. Biol. Chem., July 14, 2006; 281(28): 19124 - 19133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Gaccioliy, C. C. Huang, C. Wang, E. Bevilacqua, R. Franchi-Gazzola, G. C. Gazzola, O. Bussolati, M. D. Snider, and M. Hatzoglou Amino Acid Starvation Induces the SNAT2 Neutral Amino Acid Transporter by a Mechanism That Involves Eukaryotic Initiation Factor 2{alpha} Phosphorylation and cap-independent Translation J. Biol. Chem., June 30, 2006; 281(26): 17929 - 17940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Desvergne, L. Michalik, and W. Wahli Transcriptional Regulation of Metabolism Physiol Rev, April 1, 2006; 86(2): 465 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-X. Pan, H. Chen, and M. S. Kilberg Interaction of RNA-binding Proteins HuR and AUF1 with the Human ATF3 mRNA 3'-Untranslated Region Regulates Its Amino Acid Limitation-induced Stabilization J. Biol. Chem., October 14, 2005; 280(41): 34609 - 34616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Parker, O. P. Flint, R. Mulvey, C. Elosua, F. Wang, W. Fenderson, S. Wang, W.-P. Yang, and M. A. Noor Endoplasmic Reticulum Stress Links Dyslipidemia to Inhibition of Proteasome Activity and Glucose Transport by HIV Protease Inhibitors Mol. Pharmacol., June 1, 2005; 67(6): 1909 - 1919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. N. Roybal, L. A. Hunsaker, O. Barbash, D. L. V. Jagt, and S. F. Abcouwer The Oxidative Stressor Arsenite Activates Vascular Endothelial Growth Factor mRNA Transcription by an ATF4-dependent Mechanism J. Biol. Chem., May 27, 2005; 280(21): 20331 - 20339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhou, R. Wang, L. Fan, Y. Li, L. Ma, Z. Yang, W. Yu, N. Jing, and X. Zhu Mitosin/CENP-F as a Negative Regulator of Activating Transcription Factor-4 J. Biol. Chem., April 8, 2005; 280(14): 13973 - 13977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Jiang and R. C. Wek Phosphorylation of the {alpha}-Subunit of the Eukaryotic Initiation Factor-2 (eIF2{alpha}) Reduces Protein Synthesis and Enhances Apoptosis in Response to Proteasome Inhibition J. Biol. Chem., April 8, 2005; 280(14): 14189 - 14202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, Y.-X. Pan, E. E. Dudenhausen, and M. S. Kilberg Amino Acid Deprivation Induces the Transcription Rate of the Human Asparagine Synthetase Gene through a Timed Program of Expression and Promoter Binding of Nutrient-responsive Basic Region/Leucine Zipper Transcription Factors as Well as Localized Histone Acetylation J. Biol. Chem., December 3, 2004; 279(49): 50829 - 50839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. N. Roybal, S. Yang, C.-W. Sun, D. Hurtado, D. L. Vander Jagt, T. M. Townes, and S. F. Abcouwer Homocysteine Increases the Expression of Vascular Endothelial Growth Factor by a Mechanism Involving Endoplasmic Reticulum Stress and Transcription Factor ATF4 J. Biol. Chem., April 9, 2004; 279(15): 14844 - 14852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Gietzen, C. M. Ross, S. Hao, and J. W. Sharp Phosphorylation of eIF2{alpha} Is Involved in the Signaling of Indispensable Amino Acid Deficiency in the Anterior Piriform Cortex of the Brain in Rats J. Nutr., April 1, 2004; 134(4): 717 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Averous, A. Bruhat, C. Jousse, V. Carraro, G. Thiel, and P. Fafournoux Induction of CHOP Expression by Amino Acid Limitation Requires Both ATF4 Expression and ATF2 Phosphorylation J. Biol. Chem., February 13, 2004; 279(7): 5288 - 5297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Jiang, S. A. Wek, B. C. McGrath, D. Lu, T. Hai, H. P. Harding, X. Wang, D. Ron, D. R. Cavener, and R. C. Wek Activating Transcription Factor 3 Is Integral to the Eukaryotic Initiation Factor 2 Kinase Stress Response Mol. Cell. Biol., February 1, 2004; 24(3): 1365 - 1377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Brasse-Lagnel, A. Fairand, A. Lavoinne, and A. Husson Glutamine Stimulates Argininosuccinate Synthetase Gene Expression through Cytosolic O-Glycosylation of Sp1 in Caco-2 Cells J. Biol. Chem., December 26, 2003; 278(52): 52504 - 52510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fernandez, A. B. Lopez, C. Wang, R. Mishra, L. Zhou, I. Yaman, M. D. Snider, and M. Hatzolgou Transcriptional Control of the Arginine/Lysine Transporter, Cat-1, by Physiological Stress J. Biol. Chem., December 12, 2003; 278(50): 50000 - 50009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Claeyssens, C. Gangneux, C. Brasse-Lagnel, P. Ruminy, T. Aki, A. Lavoinne, and J.-P. Salier Amino acid control of the human glyceraldehyde 3-phosphate dehydrogenase gene transcription in hepatocyte Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G840 - G849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Pan, H. Chen, F. Siu, and M. S. Kilberg Amino Acid Deprivation and Endoplasmic Reticulum Stress Induce Expression of Multiple Activating Transcription Factor-3 mRNA Species That, When Overexpressed in HepG2 Cells, Modulate Transcription by the Human Asparagine Synthetase Promoter J. Biol. Chem., October 3, 2003; 278(40): 38402 - 38412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Luo, P. Baumeister, S. Yang, S. F. Abcouwer, and A. S. Lee Induction of Grp78/BiP by Translational Block: ACTIVATION OF THE Grp78 PROMOTER BY ATF4 THROUGH AN UPSTREAM ATF/CRE SITE INDEPENDENT OF THE ENDOPLASMIC RETICULUM STRESS ELEMENTS J. Biol. Chem., September 26, 2003; 278(39): 37375 - 37385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Averous, A. Bruhat, S. Mordier, and P. Fafournoux Recent Advances in the Understanding of Amino Acid Regulation of Gene Expression J. Nutr., June 1, 2003; 133(6): 2040S - 2045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bruhat, J. Averous, V. Carraro, C. Zhong, A. M. Reimold, M. S. Kilberg, and P. Fafournoux Differences in the Molecular Mechanisms Involved in the Transcriptional Activation of the CHOP and Asparagine Synthetase Genes in Response to Amino Acid Deprivation or Activation of the Unfolded Protein Response J. Biol. Chem., December 6, 2002; 277(50): 48107 - 48114. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |