JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M202702200 on April 26, 2002

J. Biol. Chem., Vol. 277, Issue 27, 24204-24211, July 5, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/27/24204    most recent
M202702200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Picault, N.
Right arrow Articles by Palmieri, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Picault, N.
Right arrow Articles by Palmieri, F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Identification of a Novel Transporter for Dicarboxylates and Tricarboxylates in Plant Mitochondria
BACTERIAL EXPRESSION, RECONSTITUTION, FUNCTIONAL CHARACTERIZATION, AND TISSUE DISTRIBUTION*

Nathalie PicaultDagger §, Luigi Palmieri§||, Isabella Pisano||, Michael HodgesDagger , and Ferdinando Palmieri||**

From the Dagger  Institut de Biotechnologie des Plantes, CNRS UMR8618, Université de Paris Sud, 91405 Orsay, Cedex, France and the || Department of Pharmaco-Biology, Laboratory of Biochemistry and Molecular Biology, University of Bari, Via Orabona 4, 70125 Bari, Italy

A cDNA from Arabidopsis thaliana and four related cDNAs from Nicotiana tabacum that we have isolated encode hitherto unidentified members of the mitochondrial carrier family. These proteins have been overexpressed in bacteria and reconstituted into phospholipid vesicles. Their transport properties demonstrate that they are orthologs/isoforms of a novel mitochondrial carrier capable of transporting both dicarboxylates (such as malate, oxaloacetate, oxoglutarate, and maleate) and tricarboxylates (such as citrate, isocitrate, cis-aconitate, and trans-aconitate). The newly identified dicarboxylate-tricarboxylate carrier accepts only the single protonated form of citrate (H-citrate2-) and the unprotonated form of malate (malate2-) and catalyzes obligatory, electroneutral exchanges. Oxoglutarate, citrate, and malate are mutually competitive inhibitors, showing Ki close to the respective Km. The carrier is expressed in all plant tissues examined and is largely spread in the plant kingdom. Furthermore, nitrate supply to nitrogen-starved tobacco plants leads to an increase in its mRNA in roots and leaves. The dicarboxylate-tricarboxylate carrier may play a role in important plant metabolic functions requiring organic acid flux to or from the mitochondria, such as nitrogen assimilation, export of reducing equivalents from the mitochondria, and fatty acid elongation.

* This work was supported by the Consiglio Nazionale delle Ricerche (CNR) target project on Biotechnolgy, the European Social Fund, and grants from the Minstero dell'Istruzione, dell'Universita e della Ricerca (MIUR), MIUR-CNR L.95/96, and Centro di Eccellenza di Genomica comparata, University of Bari (CEGBA).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AJ311776, AJ311777, AJ311778, AJ311779, and AJ311780.

§ These authors contributed equally to this work.

Supported by a Ministere d'Education National, de la Recherche et de la Technologie (MENRT) fellowship.

** To whom correspondence should be addressed. Tel.: 39-080-5443323; Fax: 39-080-5442770; E-mail: fpalm@farmbiol.uniba.it.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
C. P. Lee, H. Eubel, N. O'Toole, and A. H. Millar
Heterogeneity of the Mitochondrial Proteome for Photosynthetic and Non-photosynthetic Arabidopsis Metabolism
Mol. Cell. Proteomics, July 1, 2008; 7(7): 1297 - 1316.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. N. Laus, M. Soccio, D. Trono, L. Cattivelli, and D. Pastore
Plant Inner Membrane Anion Channel (PIMAC) Function in Plant Mitochondria
Plant Cell Physiol., July 1, 2008; 49(7): 1039 - 1055.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Sienkiewicz-Porzucek, A. Nunes-Nesi, R. Sulpice, J. Lisec, D. C. Centeno, P. Carillo, A. Leisse, E. Urbanczyk-Wochniak, and A. R. Fernie
Mild Reductions in Mitochondrial Citrate Synthase Activity Result in a Compromised Nitrate Assimilation and Reduced Leaf Pigmentation But Have No Effect on Photosynthetic Performance or Growth
Plant Physiology, May 1, 2008; 147(1): 115 - 127.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Palmieri, R. Arrigoni, E. Blanco, F. Carrari, M. I. Zanor, C. Studart-Guimaraes, A. R. Fernie, and F. Palmieri
Molecular Identification of an Arabidopsis S-Adenosylmethionine Transporter. Analysis of Organ Distribution, Bacterial Expression, Reconstitution into Liposomes, and Functional Characterization
Plant Physiology, November 1, 2006; 142(3): 855 - 865.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
I. Pracharoenwattana, J. E. Cornah, and S. M. Smith
Arabidopsis Peroxisomal Citrate Synthase Is Required for Fatty Acid Respiration and Seed Germination
PLANT CELL, July 1, 2005; 17(7): 2037 - 2048.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Leroch, S. Kirchberger, I. Haferkamp, M. Wahl, H. E. Neuhaus, and J. Tjaden
Identification and Characterization of a Novel Plastidic Adenine Nucleotide Uniporter from Solanum tuberosum
J. Biol. Chem., May 6, 2005; 280(18): 17992 - 18000.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
T. P. J. Dunkley, R. Watson, J. L. Griffin, P. Dupree, and K. S. Lilley
Localization of Organelle Proteins by Isotope Tagging (LOPIT)
Mol. Cell. Proteomics, November 1, 2004; 3(11): 1128 - 1134.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Vozza, E. Blanco, L. Palmieri, and F. Palmieri
Identification of the Mitochondrial GTP/GDP Transporter in Saccharomyces cerevisiae
J. Biol. Chem., May 14, 2004; 279(20): 20850 - 20857.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Jeong, S. Suh, C. Guan, Y.-F. Tsay, N. Moran, C. J. Oh, C. S. An, K. N. Demchenko, K. Pawlowski, and Y. Lee
A Nodule-Specific Dicarboxylate Transporter from Alder Is a Member of the Peptide Transporter Family
Plant Physiology, March 1, 2004; 134(3): 969 - 978.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Taniguchi, J. Nagasaki, M. Kawasaki, H. Miyake, T. Sugiyama, and M. Taniguchi
Differentiation of Dicarboxylate Transporters in Mesophyll and Bundle Sheath Chloroplasts of Maize
Plant Cell Physiol., February 15, 2004; 45(2): 187 - 200.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Pastore, S. Di Pede, and S. Passarella
Isolated Durum Wheat and Potato Cell Mitochondria Oxidize Externally Added NADH Mostly via the Malate/Oxaloacetate Shuttle with a Rate That Depends on the Carrier-Mediated Transport
Plant Physiology, December 1, 2003; 133(4): 2029 - 2039.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
J. L. Heazlewood, K. A. Howell, J. Whelan, and A. H. Millar
Towards an Analysis of the Rice Mitochondrial Proteome
Plant Physiology, May 1, 2003; 132(1): 230 - 242.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. H. Millar and J. L. Heazlewood
Genomic and Proteomic Analysis of Mitochondrial Carrier Proteins in Arabidopsis
Plant Physiology, February 1, 2003; 131(2): 443 - 453.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.