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Originally published In Press as doi:10.1074/jbc.M202198200 on May 1, 2002

J. Biol. Chem., Vol. 277, Issue 27, 24361-24367, July 5, 2002
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Role of p50/CDC37 in Hepadnavirus Assembly and Replication*

Xingtai WangDagger , Nicholas Grammatikakis§, and Jianming HuDagger

From the Dagger  Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118 and the § Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada

The cellular chaperone Hsp90 has been shown to associate with the reverse transcriptase (RT) of the duck hepatitis B virus and is required for RT functions. However, the molecular basis for the specific interaction between the RT and Hsp90 remains unknown. Comparison of protein compositional properties suggests that the RT is highly related to the protein kinase c-Raf, which interacts with Hsp90 via the cochaperone p50 (CDC37). We tested whether the RT, like c-Raf, is specifically recognized by p50. Immunoprecipitation and pull-down assays showed that p50 or p50delta C, a p50 mutant defective in Hsp90 binding, could interact specifically with the RT both in vitro and in vivo, indicating that p50 can bind the RT independently of Hsp90. Furthermore, purified p50 and p50delta C interacted directly with purified RT. The importance of p50-RT interaction for RT functions was underscored by 1) inhibition of protein-primed initiation of reverse transcription by p50delta C in vitro and 2) stimulation of viral DNA replication and RNA packaging by p50 and their inhibition by p50delta C in transfected cells. These results suggest that p50 can function as a cellular cofactor for the hepadnavirus RT by mediating the interaction between the RT and Hsp90.

* This work was supported in part by United States Public Health Service Grant R01 AI43453, a New Investigator Award of the Medical Foundation from the Harcourt General Charitable Foundation, and the American Liver Foundation (all to J. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Harcourt General Researcher and recipient of an American Liver Foundation Liver Scholar Award. To whom correspondence should be addressed: Dept. of Microbiology, Boston University School of Medicine, Rm. R516, 80 E. Concord St., Boston, MA 02118. Tel.: 617-638-4982; Fax: 617-638-4286; E-mail: jmhu@bu.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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