JBC GenomeOne product landing page

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M202198200 on May 1, 2002

J. Biol. Chem., Vol. 277, Issue 27, 24361-24367, July 5, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/27/24361    most recent
M202198200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, X.
Right arrow Articles by Hu, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, X.
Right arrow Articles by Hu, J.

Role of p50/CDC37 in Hepadnavirus Assembly and Replication*

Xingtai WangDagger , Nicholas Grammatikakis§, and Jianming HuDagger

From the Dagger  Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118 and the § Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada

The cellular chaperone Hsp90 has been shown to associate with the reverse transcriptase (RT) of the duck hepatitis B virus and is required for RT functions. However, the molecular basis for the specific interaction between the RT and Hsp90 remains unknown. Comparison of protein compositional properties suggests that the RT is highly related to the protein kinase c-Raf, which interacts with Hsp90 via the cochaperone p50 (CDC37). We tested whether the RT, like c-Raf, is specifically recognized by p50. Immunoprecipitation and pull-down assays showed that p50 or p50delta C, a p50 mutant defective in Hsp90 binding, could interact specifically with the RT both in vitro and in vivo, indicating that p50 can bind the RT independently of Hsp90. Furthermore, purified p50 and p50delta C interacted directly with purified RT. The importance of p50-RT interaction for RT functions was underscored by 1) inhibition of protein-primed initiation of reverse transcription by p50delta C in vitro and 2) stimulation of viral DNA replication and RNA packaging by p50 and their inhibition by p50delta C in transfected cells. These results suggest that p50 can function as a cellular cofactor for the hepadnavirus RT by mediating the interaction between the RT and Hsp90.

* This work was supported in part by United States Public Health Service Grant R01 AI43453, a New Investigator Award of the Medical Foundation from the Harcourt General Charitable Foundation, and the American Liver Foundation (all to J. H.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Harcourt General Researcher and recipient of an American Liver Foundation Liver Scholar Award. To whom correspondence should be addressed: Dept. of Microbiology, Boston University School of Medicine, Rm. R516, 80 E. Concord St., Boston, MA 02118. Tel.: 617-638-4982; Fax: 617-638-4286; E-mail: jmhu@bu.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 J. Virol.Home page
W. Gao and J. Hu
Formation of Hepatitis B Virus Covalently Closed Circular DNA: Removal of Genome-Linked Protein
J. Virol., June 15, 2007; 81(12): 6164 - 6174.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. H. Nguyen, S. Gummuluru, and J. Hu
Deamination-Independent Inhibition of Hepatitis B Virus Reverse Transcription by APOBEC3G
J. Virol., May 1, 2007; 81(9): 4465 - 4472.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. H. Basagoudanavar, D. H. Perlman, and J. Hu
Regulation of Hepadnavirus Reverse Transcription by Dynamic Nucleocapsid Phosphorylation
J. Virol., February 15, 2007; 81(4): 1641 - 1649.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
S.-Y. Sohn, S.-B. Kim, J. Kim, and B.-Y. Ahn
Negative regulation of hepatitis B virus replication by cellular Hsp40/DnaJ proteins through destabilization of viral core and X proteins
J. Gen. Virol., July 1, 2006; 87(7): 1883 - 1891.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Hu and M. Boyer
Hepatitis B Virus Reverse Transcriptase and {varepsilon} RNA Sequences Required for Specific Interaction In Vitro
J. Virol., March 1, 2006; 80(5): 2141 - 2150.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. H. Perlman, E. A. Berg, P. B. O'Connor, C. E. Costello, and J. Hu
Reverse transcription-associated dephosphorylation of hepadnavirus nucleocapsids
PNAS, June 21, 2005; 102(25): 9020 - 9025.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Hu, D. Flores, D. Toft, X. Wang, and D. Nguyen
Requirement of Heat Shock Protein 90 for Human Hepatitis B Virus Reverse Transcriptase Function
J. Virol., December 1, 2004; 78(23): 13122 - 13131.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Miyata and E. Nishida
CK2 Controls Multiple Protein Kinases by Phosphorylating a Kinase-Targeting Molecular Chaperone, Cdc37
Mol. Cell. Biol., May 1, 2004; 24(9): 4065 - 4074.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
P. Lee, A. Shabbir, C. Cardozo, and A. J. Caplan
Sti1 and Cdc37 Can Stabilize Hsp90 in Chaperone Complexes with a Protein Kinase
Mol. Biol. Cell, April 1, 2004; 15(4): 1785 - 1792.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Zhao, F. Boschelli, A. J. Caplan, and K. T. Arndt
Identification of a Conserved Sequence Motif That Promotes Cdc37 and Cyclin D1 Binding to Cdk4
J. Biol. Chem., March 26, 2004; 279(13): 12560 - 12564.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
X. Wang, X. Qian, H.-C. Guo, and J. Hu
Heat Shock Protein 90-Independent Activation of Truncated Hepadnavirus Reverse Transcriptase
J. Virol., April 15, 2003; 77(8): 4471 - 4480.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
P. Lee, J. Rao, A. Fliss, E. Yang, S. Garrett, and A. J. Caplan
The Cdc37 protein kinase-binding domain is sufficient for protein kinase activity and cell viability
J. Cell Biol., December 23, 2002; 159(6): 1051 - 1059.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.