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Originally published In Press as doi:10.1074/jbc.M201612200 on April 15, 2002

J. Biol. Chem., Vol. 277, Issue 27, 24530-24537, July 5, 2002
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The Novel Human DNA Helicase hFBH1 Is an F-box Protein*

Jaehoon KimDagger , Jeong-Hoon KimDagger , Sung-Hak LeeDagger , Do-Hyung KimDagger , Ho-Young KangDagger , Sung-Ho Bae§, Zhen-Qiang Pan, and Yeon-Soo SeoDagger ||

From the Dagger  National Creative Research Initiative Center for Cell Cycle Control, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, 300 Chunchun-Dong, Changan-Ku, Suwon, Kyunggi-Do, 440-746, Korea, the § Department of Pharmacology, Dong-A University College of Medicine, 3-ga, Tongdaesin-Dong, Seo-Gu, Busan, 602-103, Korea, and the  Derald H. Ruttenberg Cancer Center, The Mount Sinai School of Medicine, New York, New York 10029-6574

We have identified a novel DNA helicase in humans that belongs to members of the superfamily I helicase and found that it contains a well conserved F-box motif at its N terminus. We have named the enzyme hFBH1 (human F-box DNA helicase 1). Recombinant hFBH1, containing glutathione S-transferase at the N terminus, was expressed in Sf9 cells and purified. In this report, we show that hFBH1 exhibited DNA-dependent ATPase and DNA unwinding activities that displace duplex DNA in the 3' to 5' direction. The hFBH1 enzyme interacted with human SKP1 and formed an SCF (SKP1/Cullin/F-box) complex together with human Cullin and ROC1. In addition, the SCF complex containing hFBH1 as an F-box protein displayed ubiquitin ligase activity. We demonstrate that hFBH1 is the first F-box protein that possesses intrinsic enzyme activity. The potential role of the F-box motif and the helicase activity of the enzyme are discussed with regard to regulation of DNA metabolism.

* This work was supported by a grant from the Creative Research Initiatives of the Korean Ministry of Science and Technology (to Y.-S. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed. Tel.: 82-31-299-6440; Fax: 82-31-299-6435; E-mail: ysseo@med.skku.ac.kr.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


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