|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 28, 24926-24937, July 12, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Biochemistry, Boston University
School of Medicine, Boston and the Boston Veterans Administration
Medical Center, Boston, Massachusetts 02118
The transcription start site of the collagen
The RFX Family Interacts at the Collagen (COL1A2) Start Site and
Represses Transcription*
2(1) gene (COL1A2) has a sequence-specific
binding site for a DNA methylation-responsive binding protein called
regulatory factor for X-box 1 (RFX1) (Sengupta, P. K., Erhlich,
M., and Smith, B. D. (1999) J. Biol. Chem. 274, 36649-36655). In this report, we demonstrate that RFX1 forms
homodimers as well as heterodimers with RFX2 spanning the collagen
transcription start site. Methylation at +7 on the coding strand
increases RFX1 complex formation in gel shift assays. Methylation on
the template strand, however, does not increase RFX1 complex formation.
DNA from human fibroblasts contains minimal methylation on the coding strand (<4%) with variable methylation on the template strand. RFX1
acts as a repressor of collagen transcription as judged by in
vitro transcription and co-transfection assays with an
unmethylated collagen promoter-reporter construct. In addition, an RFX5
complex present in human fibroblasts interacts with the collagen RFX
site, which is not sensitive to methylation. This is the first
demonstration of RFX5 complex formation on a gene other than major
histocompatibility complex (MHC) promoters. Also, RFX5 represses
transcription of a collagen promoter-reporter construct in rat
fibroblasts that have no detectable RFX5 complex formation or protein.
RFX5 complex activates MHC II transcription by interacting with an
interferon-
(IFN-)-inducible protein, major histocompatibility
class II trans-activator (CIITA). Collagen transcription is repressed
by IFN- in a dose-dependent manner in human but not in
rat fibroblasts. IFN- enhances RFX5 binding activity, and CIITA is
present in the RFX5 complex of IFN--treated human fibroblasts. CIITA
repressed collagen gene transcription more effectively in human
fibroblasts than in rat fibroblasts, suggesting that the RFX5 complex
may, in part, recruit CIITA protein to the collagen transcription start
site. Thus the RFX family may be important repressors of collagen gene
transcription through a RFX binding site spanning the transcription
start site.
*
This work was supported by a Veteran Administration merit
review project and by NHLBI, National Institutes of Health Grants P01-HL56386 and R01-HL68094.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Biochemistry,
Boston University School of Medicine, 715 Albany St., Boston, MA 02118. Tel.: 617-638-4159; Fax: 617-638-5339; E-mail:
smith@biochem.bumc.bu.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Fang, X. Kong, P. Li, F. Fang, X. Wu, H. Bai, X. Qi, Q. Chen, and Y. Xu RFXB and its splice variant RFXBSV mediate the antagonism between IFN{gamma} and TGF{beta} on COL1A2 transcription in vascular smooth muscle cells Nucleic Acids Res., May 22, 2009; (2009) gkp398v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xu, J. A. Harton, and B. D. Smith CIITA Mediates Interferon-{gamma} Repression of Collagen Transcription through Phosphorylation-dependent Interactions with Co-repressor Molecules J. Biol. Chem., January 18, 2008; 283(3): 1243 - 1256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, S. R. Farmer, and B. D. Smith Peroxisome Proliferator-activated Receptor {gamma} Interacts with CIITA{middle dot}RFX5 Complex to Repress Type I Collagen Gene Expression J. Biol. Chem., September 7, 2007; 282(36): 26046 - 26056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-R. Wang, T. Nemoto, and Y. Yokota RFX1 Mediates the Serum-induced Immediate Early Response of Id2 Gene Expression J. Biol. Chem., September 7, 2007; 282(36): 26167 - 26177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, P. K. Sengupta, E. Seto, and B. D. Smith Regulatory Factor for X-box Family Proteins Differentially Interact with Histone Deacetylases to Repress Collagen {alpha}2(I) Gene (COL1A2) Expression J. Biol. Chem., April 7, 2006; 281(14): 9260 - 9270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fan, M. Zhan, J. Shen, J. L. Martindale, X. Yang, T. Kawai, and M. Gorospe En masse nascent transcription analysis to elucidate regulatory transcription factors Nucleic Acids Res., March 15, 2006; 34(5): 1492 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Buch, B. Polic, B. E. Clausen, S. Weiss, O. Akilli-Ozturk, C.-H. Chang, R. Flavell, A. Schulz, S. Jonjic, A. Waisman, et al. MHC class II expression through a hitherto unknown pathway supports T helper cell-dependent immune responses: implications for MHC class II deficiency Blood, February 15, 2006; 107(4): 1434 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Niesen, A. R. Osborne, H. Yang, S. Rastogi, S. Chellappan, J. Q. Cheng, J. M. Boss, and G. Blanck Activation of a Methylated Promoter Mediated by a Sequence-specific DNA-binding Protein, RFX J. Biol. Chem., November 25, 2005; 280(47): 38914 - 38922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Zhang and J. C. Reese Molecular Genetic Analysis of the Yeast Repressor Rfx1/Crt1 Reveals a Novel Two-Step Regulatory Mechanism Mol. Cell. Biol., September 1, 2005; 25(17): 7399 - 7411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Whitman, S. Liu, T. Vukosavljevic, L. J. Rush, L. Yu, C. Liu, M. I. Klisovic, K. Maharry, M. Guimond, M. P. Strout, et al. The MLL partial tandem duplication: evidence for recessive gain-of-function in acute myeloid leukemia identifies a novel patient subgroup for molecular-targeted therapy Blood, July 1, 2005; 106(1): 345 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sengupta, Y. Xu, L. Wang, R. Widom, and B. D. Smith Collagen {alpha}1(I) Gene (COL1A1) Is Repressed by RFX Family J. Biol. Chem., June 3, 2005; 280(22): 21004 - 21014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. C. Horvath, W. S. Kistler, and M. K. Kistler RFX2 Is a Potential Transcriptional Regulatory Factor for Histone H1t and Other Genes Expressed During the Meiotic Phase of Spermatogenesis Biol Reprod, November 1, 2004; 71(5): 1551 - 1559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, L. Wang, G. Buttice, P. K. Sengupta, and B. D. Smith Major Histocompatibility Class II Transactivator (CIITA) Mediates Repression of Collagen (COL1A2) Transcription by Interferon {gamma} (IFN-{gamma}) J. Biol. Chem., October 1, 2004; 279(40): 41319 - 41332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nozell, Z. Ma, C. Wilson, R. Shah, and E. N. Benveniste Class II Major Histocompatibility Complex Transactivator (CIITA) Inhibits Matrix Metalloproteinase-9 Gene Expression J. Biol. Chem., September 10, 2004; 279(37): 38577 - 38589. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Bonnafe, M. Touka, A. AitLounis, D. Baas, E. Barras, C. Ucla, A. Moreau, F. Flamant, R. Dubruille, P. Couble, et al. The Transcription Factor RFX3 Directs Nodal Cilium Development and Left-Right Asymmetry Specification Mol. Cell. Biol., May 15, 2004; 24(10): 4417 - 4427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. K. Yee, Y. Yao, P. Li, M. J. Klemsz, J. S. Blum, and C.-H. Chang Cathepsin E: A Novel Target for Regulation by Class II Transactivator J. Immunol., May 1, 2004; 172(9): 5528 - 5534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, L. Wang, G. Buttice, P. K. Sengupta, and B. D. Smith Interferon {gamma} Repression of Collagen (COL1A2) Transcription Is Mediated by the RFX5 Complex J. Biol. Chem., December 5, 2003; 278(49): 49134 - 49144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Higashi, Y. Inagaki, K. Fujimori, A. Nakao, H. Kaneko, and I. Nakatsuka Interferon-{gamma} Interferes with Transforming Growth Factor-{beta} Signaling through Direct Interaction of YB-1 with Smad3 J. Biol. Chem., October 31, 2003; 278(44): 43470 - 43479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. K. Sengupta, E. M. Smith, K. Kim, M. J. Murnane, and B. D. Smith DNA Hypermethylation Near the Transcription Start Site of Collagen {alpha}2(I) Gene Occurs in Both Cancer Cell Lines and Primary Colorectal Cancers Cancer Res., April 15, 2003; 63(8): 1789 - 1797. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |