Structures of Lipopolysaccharides from Klebsiella pneumoniae. ELUCIDATION OF THE STRUCTURE OF THE LINKAGE REGION BETWEEN CORE AND POLYSACCHARIDE O CHAIN AND IDENTIFICATION OF THE RESIDUES AT THE NON-REDUCING TERMINI OF THE O CHAINS

doi:10.1074/jbc.M202683200

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Structures of Lipopolysaccharides from Klebsiella pneumoniae
ELUCIDATION OF THE STRUCTURE OF THE LINKAGE REGION BETWEEN CORE AND POLYSACCHARIDE O CHAIN AND IDENTIFICATION OF THE RESIDUES AT THE NON-REDUCING TERMINI OF THE O CHAINS^*

Evgeny Vinogradov^§, Emilisa Frirdich^¶, Leann L. MacLean, Malcolm B. Perry, Bent O. Petersen^**, Jens Ø. Duus^**, and Chris Whitfield^¶

From the Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6, Canada, ^¶ Department of Microbiology, University of Guelph, Guelph, Ontario N1G 2W1, Canada, and ^** Carlsberg Laboratory, Department of Chemistry, Gamle Carlsberg Vej 10, DK-2500 Valby, Copenhagen, Denmark

Deamination of LPSs from Klebsiella pneumoniae released O-chain polysaccharides together with a fragment of the core oligosaccharide.The structures of the products from serotypes O1, O2a, O2a,c,O3, O4, O5, and O12 were determined by NMR spectroscopy and chemicalmethods, identifying the linkage region between the O antigensand the core as well as novel residues at the non-reducing endsof the polysaccharides. All serotypes had an identical linkagebetween the O chain and core.

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Structure I.

^* This work was supported by funding from the Canadian Bacterial Diseases Network (to M. B. P. and C. W.) and the Natural Sciencesand Engineering Research Council (to C. W.).The costs of publicationof this article were defrayed in part by the payment of page charges.The article must therefore be hereby marked "advertisement" inaccordance with 18 U.S.C. Section 1734 solely to indicate thisfact.

^§ To whom correspondence should be addressed: Inst. for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa,ON K1A 0R6, Canada. Tel.: 613-990-03-97; Fax: 613-952-90-92; E-mail:evguenii.vinogradov@nrc.ca.

Supported by a Postgraduate Scholarship from the Natural Sciences and Engineering ResearchCouncil.

This author acknowledges receipt of a Canada Research Chairaward.

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Structures of Lipopolysaccharides from Klebsiella pneumoniae ELUCIDATION OF THE STRUCTURE OF THE LINKAGE REGION BETWEEN CORE AND POLYSACCHARIDE O CHAIN AND IDENTIFICATION OF THE RESIDUES AT THE NON-REDUCING TERMINI OF THE O CHAINS*

Structures of Lipopolysaccharides from Klebsiella pneumoniae
ELUCIDATION OF THE STRUCTURE OF THE LINKAGE REGION BETWEEN CORE AND POLYSACCHARIDE O CHAIN AND IDENTIFICATION OF THE RESIDUES AT THE NON-REDUCING TERMINI OF THE O CHAINS^*