JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M110400200 on May 2, 2002

J. Biol. Chem., Vol. 277, Issue 28, 25115-25124, July 12, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/28/25115    most recent
M110400200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Melvin, V. S.
Right arrow Articles by Edwards, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Melvin, V. S.
Right arrow Articles by Edwards, D. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The C-terminal Extension (CTE) of the Nuclear Hormone Receptor DNA Binding Domain Determines Interactions and Functional Response to the HMGB-1/-2 Co-regulatory Proteins*

Vida Senkus MelvinDagger , Sarah C. RoemerDagger , Mair E. A. ChurchillDagger §, and Dean P. EdwardsDagger ||

From the Dagger  Program in Molecular Biology and the Departments of § Pharmacology and  Pathology, University of Colorado Health Sciences Center, Denver, Colorado 80262

Previously, we and others reported that the high mobility group proteins, HMGB-1/-2, enhance DNA binding in vitro and transactivation in situ by the steroid hormone subgroup of nuclear receptors but did not influence these functions of class II receptors. We show here that the DNA binding domain (DBD) is sufficient to account for the selective influence of HMGB-1/-2 on the steroid class of receptors. Furthermore, the use of chimeric DBDs reveals that this selectivity is dependent on the C-terminal extension (CTE), amino acid sequences adjacent to the zinc finger core DBD. HMGB-1/-2 interact directly with the DBDs of steroid but not class II receptors, and this interaction requires the CTE. This in vitro interaction correlates with a requirement of the CTE for maximal HMGB-1/-2 enhancement of DNA binding in vitro and transcriptional activation in cells. Finally, class II receptor DBDs have a much higher intrinsic affinity for DNA than steroid receptor DBDs, and this affinity difference is also dependent on the CTE. These results reveal the importance of the steroid receptor CTE for DNA binding affinity and functional response to HMGB-1/-2.

* This work was supported by National Institutes of Health Grant RO1 CA46938 (to D. P. E.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed: Dept. of Pathology, University of Colorado Health Sciences Center, 4200 E. Ninth Ave., B216, Denver, CO 80262. Tel.: 303-315-5416; Fax: 303-315-6721; E-mail: dean.edwards@uchsc.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. C. Roemer, J. Adelman, M. E. A. Churchill, and D. P. Edwards
Mechanism of high-mobility group protein B enhancement of progesterone receptor sequence-specific DNA binding
Nucleic Acids Res., June 1, 2008; 36(11): 3655 - 3666.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. R. Schultz-Norton, V. A. Gabisi, Y. S. Ziegler, I. X. McLeod, J. R. Yates, and A. M. Nardulli
Interaction of estrogen receptor {alpha} with proliferating cell nuclear antigen
Nucleic Acids Res., August 1, 2007; 35(15): 5028 - 5038.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. R. Schultz-Norton, K. A. Walt, Y. S. Ziegler, I. X. McLeod, J. R. Yates, L. T. Raetzman, and A. M. Nardulli
The Deoxyribonucleic Acid Repair Protein Flap Endonuclease-1 Modulates Estrogen-Responsive Gene Expression
Mol. Endocrinol., July 1, 2007; 21(7): 1569 - 1580.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. C. Buser, E. K. Gass-Handel, S. L. Wyszomierski, W. Doppler, S. A. Leonhardt, J. Schaack, J. M. Rosen, H. Watkin, S. M. Anderson, and D. P. Edwards
Progesterone Receptor Repression of Prolactin/Signal Transducer and Activator of Transcription 5-Mediated Transcription of the {beta}-Casein Gene in Mammary Epithelial Cells
Mol. Endocrinol., January 1, 2007; 21(1): 106 - 125.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. C. Roemer, D. C. Donham, L. Sherman, V. H. Pon, D. P. Edwards, and M. E. A. Churchill
Structure of the Progesterone Receptor-Deoxyribonucleic Acid Complex: Novel Interactions Required for Binding to Half-Site Response Elements
Mol. Endocrinol., December 1, 2006; 20(12): 3042 - 3052.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. Y. Kim, E. M. Woo, Y. T. E. Chong, D. R. Homenko, and W. L. Kraus
Acetylation of Estrogen Receptor {alpha} by p300 at Lysines 266 and 268 Enhances the Deoxyribonucleic Acid Binding and Transactivation Activities of the Receptor
Mol. Endocrinol., July 1, 2006; 20(7): 1479 - 1493.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. E. Wardell, S. C. Kwok, L. Sherman, R. S. Hodges, and D. P. Edwards
Regulation of the Amino-Terminal Transcription Activation Domain of Progesterone Receptor by a Cofactor-Induced Protein Folding Mechanism
Mol. Cell. Biol., October 15, 2005; 25(20): 8792 - 8808.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Shostak and K. R. Yamamoto
Overlapping but Separable Determinants of DNA Binding and Nuclear Localization Map to the C-terminal End of the Caenorhabditis elegans DAF-12 DNA Binding Domain
J. Biol. Chem., February 25, 2005; 280(8): 6554 - 6560.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
D. Das, R. C. Peterson, and W. M. Scovell
High Mobility Group B Proteins Facilitate Strong Estrogen Receptor Binding to Classical and Half-Site Estrogen Response Elements and Relax Binding Selectivity
Mol. Endocrinol., November 1, 2004; 18(11): 2616 - 2632.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. S. Melvin, C. Harrell, J. S. Adelman, W. L. Kraus, M. Churchill, and D. P. Edwards
The Role of the C-terminal Extension (CTE) of the Estrogen Receptor {alpha} and {beta} DNA Binding Domain in DNA Binding and Interaction with HMGB
J. Biol. Chem., April 9, 2004; 279(15): 14763 - 14771.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Li and B. W. O'Malley
Unfolding the Action of Progesterone Receptors
J. Biol. Chem., October 10, 2003; 278(41): 39261 - 39264.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G.-Z. Liu, H. Wang, and Z. Wang
Identification of a Highly Conserved Domain in the Androgen Receptor That Suppresses the DNA-binding Domain-DNA Interactions
J. Biol. Chem., April 18, 2003; 278(17): 14956 - 14960.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.