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J. Biol. Chem., Vol. 277, Issue 29, 26276-26280, July 19, 2002
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From the Institut für Genetik, Biologicum,
Martin-Luther-Universität Halle-Wittenberg, Weinbergweg
10, D-06120 Halle (Saale), Germany
The G1 arrest imposed by
Kluyveromyces lactis zymocin on Saccharomyces
cerevisiae cells requires a functional RNA polymerase II (pol II)
Elongator complex. In studying a link between zymocin and pol II,
progressively truncating the carboxyl-terminal domain (CTD) of pol II
was found to result in zymocin hypersensitivity as did mutations in
four different CTD kinase genes. Consistent with the notion that
Elongator preferentially associates with hyperphosphorylated (II0)
rather than hypophosphorylated (IIA) pol II, the II0/IIA ratio was
imbalanced toward II0 on zymocin treatment and suggests zymocin affects
pol II function, presumably in an Elongator-dependent
manner. As judged from chromatin immunoprecipitations, zymocin-arrested
cells were affected with regards to pol II binding to the
ADH1 promotor and pol II transcription of the
ADH1 gene. Thus, zymocin may interfere with pol II
recycling, a scenario assumed to lead to down-regulation of pol II
transcription and eventually causing the observed G1 arrest.
Saccharomyces cerevisiae RNA Polymerase II Is
Affected by Kluyveromyces lactis Zymocin*
and
*
This work was supported by a grant from the Deutsche
Forschungsgemeinschaft (Scha 750/2) (to R. S.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by a grant sponsored by the "Graduierten
Förderung des Landes Sachsen-Anhalt."
§
To whom correspondence should be addressed. Tel.:
49-345-5526333; Fax: 49-345-5527151; E-mail:
schaffrath@genetik.uni-halle.de.
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