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J. Biol. Chem., Vol. 277, Issue 3, 1689-1694, January 18, 2002
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From the Abscisic acid (ABA) regulates seed maturation,
germination, and adaptation of vegetative tissues to environmental
stresses. The mechanisms of ABA action and the specificity conferred by signaling components in overlapping pathways are not completely understood. The ABI5 gene (ABA
insensitive 5) of Arabidopsis
encodes a basic leucine zipper factor required for ABA response in the seed and vegetative tissues. Using transient gene expression in rice
protoplasts, we provide evidence for the functional interactions of
ABI5 with ABA signaling effectors VP1
(viviparous 1) and ABI1 (ABA insensitive 1).
Co-transformation experiments with ABI5 cDNA constructs resulted in specific transactivation of the
ABA-inducible wheat Em, Arabidopsis
AtEm6, bean
ABI5 Interacts with Abscisic Acid Signaling Effectors in
Rice Protoplasts*
,
Department of Biology, Hong Kong University
of Science and Technology, Clear Water Bay, Kowloon, Hong Kong,
China, the § Department of Molecular, Cellular, and
Developmental Biology, University of California, Santa Barbara,
Santa Barbara, California 93106, and the ¶ Department of Biology,
The Chinese University of Hong Kong, Shatin, New Territories,
Hong Kong, China
-Phaseolin, and barley
HVA1 and HVA22 promoters. Furthermore, ABI5
interacted synergistically with ABA and co-expressed VP1, indicating
that ABI5 is involved in ABA-regulated transcription mediated by VP1.
ABI5-mediated transactivation was inhibited by overexpression of
abi1-1, the dominant-negative allele of the
protein phosphatase ABI1, and by 1-butanol, a competitive inhibitor of
phospholipase D involved in ABA signaling. Lanthanum, a trivalent ion
that acts as an agonist of ABA signaling, potentiated ABI5
transactivation. These results demonstrate that ABI5 is a key target of
a conserved ABA signaling pathway in plants.
*
This work was supported by Grant AoE/B-07/99 from the Hong
Kong Government University Grants Council Area of Excellence Funding for Plant and Fungal Biotechnology.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
852-2358-8634; Fax: 852-2358-1559; E-mail: borock@ust.hk.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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