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J. Biol. Chem., Vol. 277, Issue 3, 1780-1787, January 18, 2002
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From the Institute of Plant Sciences, University of Bern,
Altenbergrain 23, Bern CH-3013, Switzerland
The behavior of purified potato mitochondria
toward the main effectors of the animal mitochondrial permeability
transition has been studied by light scattering, fluorescence,
SDS-polyacrylamide gel electrophoresis, and immunoblotting techniques.
The addition of Ca2+ induces a
phosphate-dependent swelling that is fully inhibited by
cyclosporin A if dithioerythritol is present. Mg2+ cannot
be substituted for Ca2+ but competes with it. Disruption of
the outer membrane and release of several proteins, including
cytochrome c, occur upon completion of swelling.
Ca2+-induced swelling is delayed and its rate is decreased
when pH is shifted from 7.4 to 6.6. It is accelerated by diamide,
phenylarsine oxide, and linolenic acid. In the absence of
Ca2+, however, linolenic acid (
Occurrence and Characteristics of the Mitochondrial Permeability
Transition in Plants*
,
20 µM)
rapidly dissipates the succinate-driven membrane potential while having
no effect on mitochondrial volume. Anoxic conditions favor in
vitro swelling and the concomitant release of cytochrome
c and of other proteins in a pH-dependent way.
These data indicate that the classical mitochondrial permeability transition occurs also in plants. This may have important implications for our understanding of cell stress and death processes.
*
This work was supported by the University of Bern and Swiss
National Foundation Grant 31-53722.98.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 41 31 631 49 57;
Fax: 41 31 332 20 59; E-mail: silvio.arpagaus@ips.unibe.ch.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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