JBC Oz Biosciences

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M108386200 on November 2, 2001

J. Biol. Chem., Vol. 277, Issue 3, 1912-1918, January 18, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/3/1912    most recent
M108386200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dornan, S.
Right arrow Articles by Alexander, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dornan, S.
Right arrow Articles by Alexander, D. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Differential Association of CD45 Isoforms with CD4 and CD8 Regulates the Actions of Specific Pools of p56lck Tyrosine Kinase in T Cell Antigen Receptor Signal Transduction*

Saffron DornanDagger , Zsolt Sebestyen§, John GambleDagger , Peter Nagy§, Andrea Bodnar§, Lou AlldridgeDagger , Senam Doedagger Dagger , Nick Holmes, Lindsey K. Goff||, Peter Beverley**, Janos Szollosi§, and Denis R. AlexanderDagger Dagger Dagger

From the Dagger  Laboratory of Lymphocyte Signalling and Development, Programme of Molecular Immunology, The Babraham Institute, Cambridge, CB2 4AT, United Kingdom, the § Department of Biophysics and Cell Biology, University of Debrecen, H-4012 Debrecen, Hungary, the  Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, United Kingdom, the || Imperial Cancer Research Fund Medical Oncology Unit, St. Bartholomews Hospital, Charterhouse Square, London, EC1M 6BQ, United Kingdom, and ** The Edward Jenner Institute, Compton, Newbury, Berks, RG20 7NN, United Kingdom

An investigation into the role of CD45 isoforms in T cell antigen receptor signal transduction was carried out by transfecting CD45-negative CD4+CD8+ HPB-ALL T cells with the CD45R0, CD45RBC, and CD45RABC isoforms. Fluorescence resonance energy transfer analysis showed that the CD45R0 isoform, but not the CD45RBC or CD45RABC isoforms, was found as homodimers and also preferentially associated with CD4 and CD8 at the cell-surface. A comparison was therefore made of T cell antigen receptor signaling between sub-clones expressing either CD45R0 or CD45RBC. Under basal conditions CD4-associated p56lck tyrosine kinase activity and cellular protein tyrosine phosphorylation levels were higher in the CD45R0+ than in the CD45RBC+ sub-clones. Upon CD3-CD4 ligation, TCR-zeta phosphorylation, ZAP-70 recruitment to the p21/p23 TCR-zeta phosphoisomers, ZAP-70 phosphorylation, as well as p56lck, c-Cbl and Slp-76 phosphorylation, were all markedly increased in CD45R0+ compared with CD45RBC+ cells. T cell antigen receptor (TCR) stimulation alone also promoted c-Cbl phosphorylation in CD45R0+ but not in CD45RBC+ cells. Our results are consistent with a model in which association of CD45R0 with CD4 generates a more active pool of CD4-associated p56lck kinase molecules. Upon CD3-CD4 co-ligation, the active p56lck increases the intensity of T cell antigen receptor signal transduction coupling by promoting TCR-zeta chain phosphorylation and ZAP-70 recruitment.

* This work was supported by the Biotechnology and Biological Sciences Research Council, by the British Council British-Hungarian Academic Research Program 069, and by the Hungarian Academy of Sciences Grant OKTA 30399/1999.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

dagger Senam Doe sadly died from illness since this work was carried out, and this paper is dedicated to her memory.

Dagger Dagger To whom correspondence should be addressed. Tel.: 44-1223-496554; Fax: 44-1223-496023; E-mail: Denis.Alexander@BBSRC.AC.UK.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Int ImmunolHome page
R. J. Salmond, L. McNeill, N. Holmes, and D. R. Alexander
CD4+ T cell hyper-responsiveness in CD45 transgenic mice is independent of isoform
Int. Immunol., July 1, 2008; 20(7): 819 - 827.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Filby, B. Seddon, J. Kleczkowska, R. Salmond, P. Tomlinson, M. Smida, J. A. Lindquist, B. Schraven, and R. Zamoyska
Fyn Regulates the Duration of TCR Engagement Needed for Commitment to Effector Function
J. Immunol., October 1, 2007; 179(7): 4635 - 4644.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Falahati and D. Leitenberg
Changes in the Role of the CD45 Protein Tyrosine Phosphatase in Regulating Lck Tyrosine Phosphorylation during Thymic Development
J. Immunol., February 15, 2007; 178(4): 2056 - 2064.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Dawes, S. Petrova, Z. Liu, D. Wraith, P. C. L. Beverley, and E. Z. Tchilian
Combinations of CD45 Isoforms Are Crucial for Immune Function and Disease
J. Immunol., March 15, 2006; 176(6): 3417 - 3425.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Maksumova, H. T. Le, F. Muratkhodjaev, D. Davidson, A. Veillette, and C. J. Pallen
Protein Tyrosine Phosphatase {alpha} Regulates Fyn Activity and Cbp/PAG Phosphorylation in Thymocyte Lipid Rafts
J. Immunol., December 15, 2005; 175(12): 7947 - 7956.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Tong, J. Nguyen, and K. W. Lynch
Differential Expression of CD45 Isoforms Is Controlled by the Combined Activity of Basal and Inducible Splicing-regulatory Elements in Each of the Variable Exons
J. Biol. Chem., November 18, 2005; 280(46): 38297 - 38304.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F.-J. Li, N. Tsuyama, H. Ishikawa, M. Obata, S. Abroun, S. Liu, K.-i. Otsuyama, X. Zheng, Z. Ma, Y. Maki, et al.
A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma
Blood, April 15, 2005; 105(8): 3295 - 3302.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Wang and P. Johnson
Expression of CD45 Lacking the Catalytic Protein Tyrosine Phosphatase Domain Modulates Lck Phosphorylation and T Cell Activation
J. Biol. Chem., April 8, 2005; 280(14): 14318 - 14324.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Shimizu, R. Iida, Y. Sato, E. Moriizumi, A. Nishikawa, and Y. Ishida
Cross-Linking of CD45 on Suppressive/Regulatory T Cells Leads to the Abrogation of Their Suppressive Activity In Vitro
J. Immunol., April 1, 2005; 174(7): 4090 - 4097.
[Abstract] [Full Text] [PDF]

Home page
 LupusHome page
E C Jury and P S Kabouridis
T-lymphocyte signalling in systemic lupus erythematosus: a lipid raft perspective
Lupus, June 1, 2004; 13(6): 413 - 422.
[Abstract] [PDF]

Home page
 BloodHome page
A. Takeda, A. Matsuda, R. M. J. Paul, and N. R. Yaseen
CD45-associated protein inhibits CD45 dimerization and up-regulates its protein tyrosine phosphatase activity
Blood, May 1, 2004; 103(9): 3440 - 3447.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Ogilvy, C. Louis-Dit-Sully, J. Cooper, R. L. Cassady, D. R. Alexander, and N. Holmes
Either of the CD45RB and CD45RO Isoforms Are Effective in Restoring T Cell, But Not B Cell, Development and Function in CD45-Null Mice
J. Immunol., August 15, 2003; 171(4): 1792 - 1800.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Stanton, S. Boxall, K. Hirai, R. Dawes, S. Tonks, T. Yasui, Y. Kanaoka, N. Yuldasheva, O. Ishiko, W. Bodmer, et al.
A high-frequency polymorphism in exon 6 of the CD45 tyrosine phosphatase gene (PTPRC) resulting in altered isoform expression
PNAS, May 13, 2003; 100(10): 5997 - 6002.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. van der Wijk, C. Blanchetot, J. Overvoorde, and J. den Hertog
Redox-regulated Rotational Coupling of Receptor Protein-tyrosine Phosphatase alpha Dimers
J. Biol. Chem., April 11, 2003; 278(16): 13968 - 13974.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Harding, P. Lipp, and D. R. Alexander
A Therapeutic CD4 Monoclonal Antibody Inhibits TCR-{zeta} Chain Phosphorylation, {zeta}-Associated Protein of 70-kDa Tyr319 Phosphorylation, and TCR Internalization in Primary Human T Cells
J. Immunol., July 1, 2002; 169(1): 230 - 238.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. A. Robichaud, B. Barbeau, J.-F. Fortin, D. M. Rothstein, and M. J. Tremblay
Nuclear Factor of Activated T Cells Is a Driving Force for Preferential Productive HIV-1 Infection of CD45RO-expressing CD4+ T Cells
J. Biol. Chem., June 21, 2002; 277(26): 23733 - 23741.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.