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J. Biol. Chem., Vol. 277, Issue 30, 26753-26760, July 26, 2002
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From the Departments of Two genes, fruA and csgA,
encoding a putative transcription factor and C-factor, respectively,
are essential for fruiting body formation of Myxococcus
xanthus. To investigate the role of fruA and
csgA genes in developmental gene expression, developing cells as well as vegetative cells of M. xanthus wild-type,
fruA::Tc, and csgA731 strains were
pulse-labeled with [35S]methionine, and the whole cell
proteins were analyzed using two-dimensional immobilized pH
gradient/SDS-PAGE. Differences in protein synthesis patterns among more
than 700 protein spots were detected during development of the three
strains. Fourteen proteins showing distinctly different expression
patterns in mutant cells were analyzed in more detail. Five of the 14 proteins were identified as elongation factor Tu (EF-Tu), Dru,
DofA, FruA, and protein S by immunoblot analysis and mass spectroscopy.
A gene encoding DofA was cloned and sequenced. Although both
fruA and csgA genes regulate early development
of M. xanthus, they were found to differently regulate
expression of several developmental genes. The production of six
proteins, including DofA and protein S, was dependent on
fruA, whereas the production of two proteins was dependent
on csgA, and one protein was dependent on both
fruA and csgA. To explain the present findings,
a new model was presented in which different levels of FruA
phosphorylation may distinctively regulate the expression of two groups
of developmental genes.
Role of fruA and csgA Genes in Gene
Expression during Development of Myxococcus xanthus
ANALYSIS BY TWO-DIMENSIONAL GEL ELECTROPHORESIS*,
§,
, and Biology and
¶ Chemistry, Tokyo Metropolitan University, Minamiohsawa,
Hachioji, Tokyo 192-0397, Japan and the § Department of
Biochemistry, Robert Wood Johnson Medical School,
Piscataway, New Jersey 08854
*
This work was supported in part by a grant from the Ministry
of Education, Science, Sports and Culture of Japan and by the Foundation of Medicine and Dentistry of New Jersey.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
The on-line version of this article (available at
http://www.jbc.org) contains the results of a detailed analysis of Fig. 1.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AB073986 and AB073987.
To whom correspondence may be addressed. Tel.:
81-426-77-2568; Fax: 81-426-77-2559; E-mail:
komano-teruya@c.metro-u.ac.jp.
**
To whom correspondence may be addressed: Dept. of Biochemistry,
Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ
08854. Tel.: 732-235-4161; Fax: 732-235-4783, E-mail:
sinouye@waksman.rutgers.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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