JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M203887200 on May 9, 2002

J. Biol. Chem., Vol. 277, Issue 30, 27200-27209, July 26, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/30/27200    most recent
M203887200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Back, S. H.
Right arrow Articles by Jang, S. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Back, S. H.
Right arrow Articles by Jang, S. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Polypyrimidine Tract-binding Proteins Are Cleaved by Caspase-3 during Apoptosis*

Sung Hoon Back§, Sejeong Shin, and Sung Key JangDagger §

From the § National Research Laboratory and the  National Creative Research Center for Biomolecular Interaction, Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San31, Hyoja-Dong, Pohang, Kyungbuk 790-784, Korea

The polypyrimidine tract-binding protein (PTB), an RNA-binding protein, is required for efficient translation of some mRNAs containing internal ribosomal entry sites (IRESs). Here we provide evidence that the addition of apoptosis-inducing agents to cells results in the cleavage of PTB isoforms 1, 2, and 4 by caspase-3. This cleavage of PTB separated the N-terminal region, containing NLS-RRM1, from the C-terminal region, containing RRM2-3-4. Our data indicate that there are three noncanonical caspase-3 target sites in PTBs, namely Ile-Val-Pro-Asp7down-arrow Ile, Leu-Tyr-Thr-Asp139down-arrow Ser, and Ala-Ala-Val-Asp172down-arrow Ala. The C-terminal PTB fragments localized to the cytoplasm, as opposed to the nucleus where most intact PTBs are found. Moreover, these C-terminal PTB fragments inhibited translation of polioviral mRNA, which contains an IRES element requiring PTB for its activation. This suggests that translation of some IRES-containing mRNAs is regulated by proteolytic cleavage of PTB during apoptosis.

* This work was supported in part by the G7, National Research Laboratory, and Molecular Medicine Research Group Programs of the Ministry of Science and Technology and by a grant from the Korea Science and Engineering Foundation through the Protein Network Research Center.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San31, Hyoja-Dong, Pohang, Kyungbuk 790-784, Korea. Tel.: 82-54-279-2298; Fax: 82-54-279-8009; E-mail: sungkey@postech.ac.kr.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Molecular Cancer TherapeuticsHome page
L. Shkreta, U. Froehlich, E. R. Paquet, J. Toutant, S. A. Elela, and B. Chabot
Anticancer drugs affect the alternative splicing of Bcl-x and other human apoptotic genes
Mol. Cancer Ther., June 1, 2008; 7(6): 1398 - 1409.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
N. Park, P. Katikaneni, T. Skern, and K. E. Gustin
Differential Targeting of Nuclear Pore Complex Proteins in Poliovirus-Infected Cells
J. Virol., February 15, 2008; 82(4): 1647 - 1655.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. D. Baird, M. Turcotte, R. G. Korneluk, and M. Holcik
Searching for IRES
RNA, October 1, 2006; 12(10): 1755 - 1785.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
R. Adair, G. W. Liebisch, Y. Su, and A. M. Colberg-Poley
Alteration of cellular RNA splicing and polyadenylation machineries during productive human cytomegalovirus infection
J. Gen. Virol., December 1, 2004; 85(12): 3541 - 3553.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Choi, J. H. Kim, X. Li, K. Y. Paek, S. H. Ha, S. H. Ryu, E. Wimmer, and S. K. Jang
Identification of cellular proteins enhancing activities of internal ribosomal entry sites by competition with oligodeoxynucleotides
Nucleic Acids Res., February 23, 2004; 32(4): 1308 - 1317.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
R. Feuer, I. Mena, R. R. Pagarigan, S. Harkins, D. E. Hassett, and J. L. Whitton
Coxsackievirus B3 and the Neonatal CNS: The Roles of Stem Cells, Developing Neurons, and Apoptosis in Infection, Viral Dissemination, and Disease
Am. J. Pathol., October 1, 2003; 163(4): 1379 - 1393.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
J. VYAS, A. ELIA, and M. J. CLEMENS
Inhibition of the protein kinase PKR by the internal ribosome entry site of hepatitis C virus genomic RNA
RNA, July 1, 2003; 9(7): 858 - 870.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Gooding, P. Kemp, and C. W. J. Smith
A Novel Polypyrimidine Tract-binding Protein Paralog Expressed in Smooth Muscle Cells
J. Biol. Chem., April 18, 2003; 278(17): 15201 - 15207.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.