| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 31, 27697-27705, August 2, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Cutaneous Biology Research Center, Massachusetts General
Hospital and Harvard Medical School,
Charlestown, Massachusetts 02129
Ikaros is a key regulator of the hemo-lymphoid
system in which it is presumed to function by both potentiating and
repressing gene expression. Repression is mediated through two
independent domains at the N and C terminus of the protein, both of
which can independently recruit the corepressors Mi-2
A Molecular Dissection of the Repression Circuitry of Ikaros*
, Sin3A, and
Sin3B and the Class I histone deacetylases 1 and 2; the
N-terminal domain can also associate with the corepressor CtBP. Here we
describe a detailed dissection of these two domains and identify the
minimal repression modules and the corepressor requirements for their activity. Based on these studies, we describe mutations in a
full-length Ikaros protein that abrogate interactions with each of the
identified corepressors and abolish the protein's function as a
repressor. Finally, we show that, barring CtBP, the Ikaros family
members Aiolos, Helios, and Eos can associate with all of the
identified corepressors of Ikaros including its newly identified
interactors, Class II HDACs.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by National Institutes of Health Grant RO1-AI380342-08.
To whom correspondence should be addressed: CBRC, MGH East, Bldg. 149, 13th St., Charlestown, MA 02129. Tel.: 617-726-4445; Fax: 617-726-4453;
E-mail: katia.georgopoulos@cbrc2.mgh.harvard.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Sridharan and S. T. Smale Predominant Interaction of Both Ikaros and Helios with the NuRD Complex in Immature Thymocytes J. Biol. Chem., October 12, 2007; 282(41): 30227 - 30238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Caballero, F. Setien, L. Lopez-Serra, M. Boix-Chornet, M. F. Fraga, S. Ropero, D. Megias, M. Alaminos, E. M. Sanchez-Tapia, M. C. Montoya, et al. Combinatorial effects of splice variants modulate function of Aiolos J. Cell Sci., August 1, 2007; 120(15): 2619 - 2630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Hu, S. M. Sharma, A. Bronisz, R. Srinivasan, U. Sankar, and M. C. Ostrowski Eos, MITF, and PU.1 Recruit Corepressors to Osteoclast-Specific Genes in Committed Myeloid Progenitors Mol. Cell. Biol., June 1, 2007; 27(11): 4018 - 4027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bandyopadhyay, M. Dure, M. Paroder, N. Soto-Nieves, I. Puga, and F. Macian Interleukin 2 gene transcription is regulated by Ikaros-induced changes in histone acetylation in anergic T cells Blood, April 1, 2007; 109(7): 2878 - 2886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. R. Quinlan, A. Verger, A. Kwok, S. H. Y. Lee, J. Perdomo, M. Nardini, M. Bolognesi, and M. Crossley Role of the C-Terminal Binding Protein PXDLS Motif Binding Cleft in Protein Interactions and Transcriptional Repression Mol. Cell. Biol., November 1, 2006; 26(21): 8202 - 8213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Oswald, M. Winkler, Y. Cao, K. Astrahantseff, S. Bourteele, W. Knochel, and T. Borggrefe RBP-J{kappa}/SHARP Recruits CtIP/CtBP Corepressors To Silence Notch Target Genes Mol. Cell. Biol., December 1, 2005; 25(23): 10379 - 10390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Westman, J. Perdomo, M. Sunde, M. Crossley, and J. P. Mackay The C-terminal Domain of Eos Forms a High Order Complex in Solution J. Biol. Chem., October 24, 2003; 278(43): 42419 - 42426. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
