Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M203236200 on May 21, 2002

J. Biol. Chem., Vol. 277, Issue 31, 27818-27828, August 2, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/31/27818    most recent
M203236200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gladwin, M. T.
Right arrow Articles by Schechter, A. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gladwin, M. T.
Right arrow Articles by Schechter, A. N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

S-Nitrosohemoglobin Is Unstable in the Reductive Erythrocyte Environment and Lacks O2/NO-linked Allosteric Function*

Mark T. GladwinDagger §, Xunde WangDagger §, Christopher D. ReiterDagger §, Benjamin K. YangDagger , Esther X. VivasDagger §, Celia Bonaventura||, and Alan N. Schechter§

From the Dagger  Critical Care Medicine Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892, the § Laboratory of Chemical Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, and the || Marine/Freshwater Biomedical Center, Duke University, Beaufort, North Carolina 28516

Our previous results run counter to the hypothesis that S-nitrosohemoglobin (SNO-Hb) serves as an in vivo reservoir for NO from which NO release is allosterically linked to oxygen release. We show here that SNO-Hb undergoes reductive decomposition in erythrocytes, whereas it is stable in purified solutions and in erythrocyte lysates treated with an oxidant such as ferricyanide. Using an extensively validated methodology that eliminates background nitrite and stabilizes erythrocyte S-nitrosothiols, we find the levels of SNO-Hb in the basal human circulation, including red cell membrane fractions, were 46 ± 17 nM in human arterial erythrocytes and 69 ± 11 nM in venous erythrocytes, incompatible with the postulated reservoir function of SNO-Hb. Moreover, we performed experiments on human red blood cells in which we elevated the levels of SNO-Hb to 10,000 times the normal in vivo levels. The elevated levels of intra-erythrocytic SNO-Hb fell rapidly, independent of oxygen tension and hemoglobin saturation. Most of the NO released during this process was oxidized to nitrate. A fraction (25%) was exported as S-nitrosothiol, but this fraction was not increased at low oxygen tensions that favor the deoxy (T-state) conformation of Hb. Results of these studies show that, within the redox-active erythrocyte environment, the beta -globin cysteine 93 is maintained in a reduced state, necessary for normal oxygen affinity, and incapable of oxygen-linked NO storage and delivery.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom all correspondence and reprint requests should be addressed: Warren G. Magnuson Clinical Center, Critical Care Medicine Dept., Bldg. 10, Rm. 7D43, 10 Center Dr., MSC 1662, Bethesda, MD 20892-1662. Tel.: 301-496-9320; Fax: 301-402-1213; E-mail: mgladwin@nih.gov.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. T. Salgado, E. Nagababu, and J. M. Rifkind
Quantification of Intermediates Formed during the Reduction of Nitrite by Deoxyhemoglobin
J. Biol. Chem., May 8, 2009; 284(19): 12710 - 12718.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Feelisch, B. O. Fernandez, N. S. Bryan, M. F. Garcia-Saura, S. Bauer, D. R. Whitlock, P. C. Ford, D. R. Janero, J. Rodriguez, and H. Ashrafian
Tissue Processing of Nitrite in Hypoxia: AN INTRICATE INTERPLAY OF NITRIC OXIDE-GENERATING AND -SCAVENGING SYSTEMS
J. Biol. Chem., December 5, 2008; 283(49): 33927 - 33934.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. T. Gladwin and D. B. Kim-Shapiro
The functional nitrite reductase activity of the heme-globins
Blood, October 1, 2008; 112(7): 2636 - 2647.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. R. Jarboe, D. R. Hyduke, L. M. Tran, K. J. Y. Chou, and J. C. Liao
Determination of the Escherichia coli S-Nitrosoglutathione Response Network Using Integrated Biochemical and Systems Analysis
J. Biol. Chem., February 22, 2008; 283(8): 5148 - 5157.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. C. Rogers, A. Khalatbari, B. N. Datta, S. Ellery, V. Paul, M. P. Frenneaux, and P. E. James
NO metabolite flux across the human coronary circulation
Cardiovasc Res, July 15, 2007; 75(2): 434 - 441.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Grubina, Z. Huang, S. Shiva, M. S. Joshi, I. Azarov, S. Basu, L. A. Ringwood, A. Jiang, N. Hogg, D. B. Kim-Shapiro, et al.
Concerted Nitric Oxide Formation and Release from the Simultaneous Reactions of Nitrite with Deoxy- and Oxyhemoglobin
J. Biol. Chem., April 27, 2007; 282(17): 12916 - 12927.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
P. Sonveaux, I. I. Lobysheva, O. Feron, and T. J. McMahon
Transport and Peripheral Bioactivities of Nitrogen Oxides Carried by Red Blood Cell Hemoglobin: Role in Oxygen Delivery
Physiology, April 1, 2007; 22(2): 97 - 112.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Hausladen, R. Rafikov, M. Angelo, D. J. Singel, E. Nudler, and J. S. Stamler
Assessment of nitric oxide signals by triiodide chemiluminescence
PNAS, February 13, 2007; 104(7): 2157 - 2162.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Doctor, B. Gaston, and D. B. Kim-Shapiro
Detecting physiologic fluctuations in the S-nitrosohemoglobin micropopulation: triiodide versus 3C.
Blood, November 1, 2006; 108(9): 3225 - 3227.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. T. Gladwin, N. J. H. Raat, S. Shiva, C. Dezfulian, N. Hogg, D. B. Kim-Shapiro, and R. P. Patel
Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection, and vasodilation
Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2026 - H2035.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Wang, N. S. Bryan, P. H. MacArthur, J. Rodriguez, M. T. Gladwin, and M. Feelisch
Measurement of Nitric Oxide Levels in the Red Cell: VALIDATION OF TRI-IODIDE-BASED CHEMILUMINESCENCE WITH ACID-SULFANILAMIDE PRETREATMENT
J. Biol. Chem., September 15, 2006; 281(37): 26994 - 27002.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
E. B. Manukhina, H. F. Downey, and R. T. Mallet
Role of nitric oxide in cardiovascular adaptation to intermittent hypoxia.
Experimental Biology and Medicine, April 1, 2006; 231(4): 343 - 365.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
D. B. Kim-Shapiro, A. N. Schechter, and M. T. Gladwin
Unraveling the Reactions of Nitric Oxide, Nitrite, and Hemoglobin in Physiology and Therapeutics
Arterioscler. Thromb. Vasc. Biol., April 1, 2006; 26(4): 697 - 705.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. T. Huang, I. Azarov, S. Basu, J. Huang, and D. B. Kim-Shapiro
Lack of allosterically controlled intramolecular transfer of nitric oxide from the heme to cysteine in the beta subunit of hemoglobin
Blood, April 1, 2006; 107(7): 2602 - 2604.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Piknova, M. T. Gladwin, A. N. Schechter, and N. Hogg
Electron Paramagnetic Resonance Analysis of Nitrosylhemoglobin in Humans during NO Inhalation
J. Biol. Chem., December 9, 2005; 280(49): 40583 - 40588.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. C. Rogers, A. Khalatbari, P. W. Gapper, M. P. Frenneaux, and P. E. James
Detection of Human Red Blood Cell-bound Nitric Oxide
J. Biol. Chem., July 22, 2005; 280(29): 26720 - 26728.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Dejam, C. J. Hunter, M. M. Pelletier, L. L. Hsu, R. F. Machado, S. Shiva, G. G. Power, M. Kelm, M. T. Gladwin, and A. N. Schechter
Erythrocytes are the major intravascular storage sites of nitrite in human blood
Blood, July 15, 2005; 106(2): 734 - 739.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. J. Gow
Nitric Oxide, Hemoglobin, and Hypoxic Vasodilation
Am. J. Respir. Cell Mol. Biol., June 1, 2005; 32(6): 479 - 482.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. J. Schopfer, P. R. S. Baker, G. Giles, P. Chumley, C. Batthyany, J. Crawford, R. P. Patel, N. Hogg, B. P. Branchaud, J. R. Lancaster Jr., et al.
Fatty Acid Transduction of Nitric Oxide Signaling: NITROLINOLEIC ACID IS A HYDROPHOBICALLY STABILIZED NITRIC OXIDE DONOR
J. Biol. Chem., May 13, 2005; 280(19): 19289 - 19297.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Doctor, R. Platt, M. L. Sheram, A. Eischeid, T. McMahon, T. Maxey, J. Doherty, M. Axelrod, J. Kline, M. Gurka, et al.
Hemoglobin conformation couples erythrocyte S-nitrosothiol content to O2 gradients
PNAS, April 19, 2005; 102(16): 5709 - 5714.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
R. M. Pluta, A. Dejam, G. Grimes, M. T. Gladwin, and E. H. Oldfield
Nitrite Infusions to Prevent Delayed Cerebral Vasospasm in a Primate Model of Subarachnoid Hemorrhage
JAMA, March 23, 2005; 293(12): 1477 - 1484.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Yang and J. Loscalzo
S-nitrosoprotein formation and localization in endothelial cells
PNAS, January 4, 2005; 102(1): 117 - 122.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Deem, S. S. Kim, J.-H. Min, R. Eveland, J. Moulding, S. Martyr, X. Wang, E. R. Swenson, and M. T. Gladwin
Pulmonary vascular effects of red blood cells containing S-nitrosated hemoglobin
Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2561 - H2568.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Fago, C. Hundahl, S. Dewilde, K. Gilany, L. Moens, and R. E. Weber
Allosteric Regulation and Temperature Dependence of Oxygen Binding in Human Neuroglobin and Cytoglobin: MOLECULAR MECHANISMS AND PHYSIOLOGICAL SIGNIFICANCE
J. Biol. Chem., October 22, 2004; 279(43): 44417 - 44426.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. H. Crawford, B. K. Chacko, H. M. Pruitt, B. Piknova, N. Hogg, and R. P. Patel
Transduction of NO-bioactivity by the red blood cell in sepsis: novel mechanisms of vasodilation during acute inflammatory disease
Blood, September 1, 2004; 104(5): 1375 - 1382.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. T. Gladwin and A. N. Schechter
NO Contest: Nitrite Versus S-Nitroso-Hemoglobin
Circ. Res., April 16, 2004; 94(7): 851 - 855.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. S. Bryan, T. Rassaf, R. E. Maloney, C. M. Rodriguez, F. Saijo, J. R. Rodriguez, and M. Feelisch
Cellular targets and mechanisms of nitros(yl)ation: An insight into their nature and kinetics in vivo
PNAS, March 23, 2004; 101(12): 4308 - 4313.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. H. Han, E. Qamirani, A. G. Nelson, D. R. Hyduke, G. Chaudhuri, L. Kuo, and J. C. Liao
Regulation of nitric oxide consumption by hypoxic red blood cells
PNAS, October 14, 2003; 100(21): 12504 - 12509.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Xu, M. Cho, N. Y. Spencer, N. Patel, Z. Huang, H. Shields, S. B. King, M. T. Gladwin, N. Hogg, and D. B. Kim-Shapiro
Measurements of nitric oxide on the heme iron and {beta}-93 thiol of human hemoglobin during cycles of oxygenation and deoxygenation
PNAS, September 30, 2003; 100(20): 11303 - 11308.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
J. S. Stamler, B. M. Gaston, J. M. Hare, T. J. McMahon, J. R. Pawloski, D. J. Singel, A. N. Schechter, and M. T. Gladwin
Hemoglobin and Nitric Oxide
N. Engl. J. Med., July 24, 2003; 349(4): 402 - 405.
[Full Text] [PDF]

Home page
 Infect. Immun.Home page
S. Luckhart, A. L. Crampton, R. Zamora, M. J. Lieber, P. C. Dos Santos, T. M. L. Peterson, N. Emmith, J. Lim, D. A. Wink, and Y. Vodovotz
Mammalian Transforming Growth Factor {beta}1 Activated after Ingestion by Anopheles stephensi Modulates Mosquito Immunity
Infect. Immun., June 1, 2003; 71(6): 3000 - 3009.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. H. Crawford, C. R. White, and R. P. Patel
Vasoactivity of S-nitrosohemoglobin: role of oxygen, heme, and NO oxidation states
Blood, June 1, 2003; 101(11): 4408 - 4415.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
A. N. Schechter and M. T. Gladwin
Hemoglobin and the Paracrine and Endocrine Functions of Nitric Oxide
N. Engl. J. Med., April 10, 2003; 348(15): 1483 - 1485.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Herold and G. Rock
Reactions of Deoxy-, Oxy-, and Methemoglobin with Nitrogen Monoxide. MECHANISTIC STUDIES OF THE S-NITROSOTHIOL FORMATION UNDER DIFFERENT MIXING CONDITIONS
J. Biol. Chem., February 21, 2003; 278(9): 6623 - 6634.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Rodriguez, R. E. Maloney, T. Rassaf, N. S. Bryan, and M. Feelisch
Chemical nature of nitric oxide storage forms in rat vascular tissue
PNAS, January 7, 2003; 100(1): 336 - 341.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
T. Rassaf, P. Kleinbongard, M. Preik, A. Dejam, P. Gharini, T. Lauer, J. Erckenbrecht, A. Duschin, R. Schulz, G. Heusch, et al.
Plasma Nitrosothiols Contribute to the Systemic Vasodilator Effects of Intravenously Applied NO: Experimental and Clinical Study on the Fate of NO in Human Blood
Circ. Res., September 20, 2002; 91(6): 470 - 477.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement