| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 32, 28380-28383, August 9, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
and
From the Department of Biology, Graduate School of Science,
Osaka University, Toyonaka, Osaka 560-0043, Japan
The assembly of iron-sulfur (Fe-S) clusters is
mediated by complex machinery. In several proteobacteria, this process
involves ISC (Fe-S cluster assembly) machinery composed of at least six components also conserved in mitochondria from lower to higher eukaryotes. In nitrogen-fixing bacteria, another system, termed NIF
(nitrogen fixation), is required for the maturation of nitrogenase. Here we report the identification of a third system, designated the SUF
machinery, the components of which are encoded in Escherichia coli by an unassigned operon, sufABCDSE. We have
analyzed spontaneous pseudorevertants isolated from a mutant strain
lacking all the components of the ISC machinery. The suppressor
mutations in the revertants have been localized to the regulatory
region of the suf operon; overexpression of this operon
restores the growth phenotypes and activity of Fe-S proteins in mutant
cells lacking ISC. Disruption of the suf operon alone does
not cause any major defects, but synthetic lethality was observed when
both the isc and suf operons were inactivated.
These results indicate that proteins encoded by the suf
operon participate in the ISC-independent minor pathway for the
assembly of Fe-S clusters. The genes homologous to sufBC
are present in a wide range of bacteria, Archaea, and plastids,
suggesting that this type of system is almost ubiquitous in nature.
The on-line version of this article (available at
http://www.jbc.org) contains Supplemental Tables SI and SII and Fig. S1.
To whom correspondence should be addressed. Tel.: 81-6-6850-5423;
Fax: 81-6-6850-5425; E-mail: ytaka@bio.sci.osaka-u.ac.jp.
This article has been cited by other articles:
|
|
 |
|
  T. W. Overton, M. C. Justino, Y. Li, J. M. Baptista, A. M. P. Melo, J. A. Cole, and L. M. Saraiva Widespread Distribution in Pathogenic Bacteria of Di-Iron Proteins That Repair Oxidative and Nitrosative Damage to Iron-Sulfur Centers J. Bacteriol., March 15, 2008; 190(6): 2004 - 2013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ayala-Castro, A. Saini, and F. W. Outten Fe-S Cluster Assembly Pathways in Bacteria Microbiol. Mol. Biol. Rev., March 1, 2008; 72(1): 110 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Runyen-Janecky, A. Daugherty, B. Lloyd, C. Wellington, H. Eskandarian, and M. Sagransky Role and Regulation of Iron-Sulfur Cluster Biosynthesis Genes in Shigella flexneri Virulence Infect. Immun., March 1, 2008; 76(3): 1083 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shimomura, H. Kamikubo, Y. Nishi, T. Masako, M. Kataoka, Y. Kobayashi, K. Fukuyama, and Y. Takahashi Characterization and Crystallization of an IscU-type Scaffold Protein with Bound [2Fe 2S] Cluster from the Hyperthermophile, Aquifex aeolicus J. Biochem., November 1, 2007; 142(5): 577 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nakunst, C. Larisch, A. T. Huser, A. Tauch, A. Puhler, and J. Kalinowski The Extracytoplasmic Function-Type Sigma Factor SigM of Corynebacterium glutamicum ATCC 13032 Is Involved in Transcription of Disulfide Stress-Related Genes J. Bacteriol., July 1, 2007; 189(13): 4696 - 4707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. M. U. M., S. Ollagnier-de-Choudens, Y. Sanakis, S. E. Abdel-Ghany, C. Rousset, H. Ye, M. Fontecave, E. A. H. Pilon-Smits, and M. Pilon Characterization of Arabidopsis thaliana SufE2 and SufE3: FUNCTIONS IN CHLOROPLAST IRON-SULFUR CLUSTER ASSEMBLY AND NAD SYNTHESIS J. Biol. Chem., June 22, 2007; 282(25): 18254 - 18264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Layer, S. A. Gaddam, C. N. Ayala-Castro, S. Ollagnier-de Choudens, D. Lascoux, M. Fontecave, and F. W. Outten SufE Transfers Sulfur from SufS to SufB for Iron-Sulfur Cluster Assembly J. Biol. Chem., May 4, 2007; 282(18): 13342 - 13350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Justino, C. C. Almeida, M. Teixeira, and L. M. Saraiva Escherichia coli Di-iron YtfE Protein Is Necessary for the Repair of Stress-damaged Iron-Sulfur Clusters J. Biol. Chem., April 6, 2007; 282(14): 10352 - 10359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Van Hoewyk, S. E. Abdel-Ghany, C. M. Cohu, S. K. Herbert, P. Kugrens, M. Pilon, and E. A. H. Pilon-Smits Chloroplast iron-sulfur cluster protein maturation requires the essential cysteine desulfurase CpNifS PNAS, March 27, 2007; 104(13): 5686 - 5691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Johnson, M.-C. Unciuleac, and D. R. Dean Controlled Expression and Functional Analysis of Iron-Sulfur Cluster Biosynthetic Components within Azotobacter vinelandii J. Bacteriol., November 1, 2006; 188(21): 7551 - 7561. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Li, W.-H. Tong, R. M. Hughes, and T. A. Rouault Roles of the Mammalian Cytosolic Cysteine Desulfurase, ISCS, and Scaffold Protein, ISCU, in Iron-Sulfur Cluster Assembly J. Biol. Chem., May 5, 2006; 281(18): 12344 - 12351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Balasubramanian, G. Shen, D. A. Bryant, and J. H. Golbeck Regulatory Roles for IscA and SufA in Iron Homeostasis and Redox Stress Responses in the Cyanobacterium Synechococcus sp. Strain PCC 7002 J. Bacteriol., May 1, 2006; 188(9): 3182 - 3191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Malone, Y.-K. Chung, and A. E. Yousef Genes of Escherichia coli O157:H7 That Are Involved in High-Pressure Resistance Appl. Envir. Microbiol., April 1, 2006; 72(4): 2661 - 2671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Eccleston, A. Petrovic, C. T. Davis, K. Rangachari, and R. J. M. Wilson The Kinetic Mechanism of the SufC ATPase: THE CLEAVAGE STEP IS ACCELERATED BY SufB J. Biol. Chem., March 31, 2006; 281(13): 8371 - 8378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ye, S. E. Abdel-Ghany, T. D. Anderson, E. A. H. Pilon-Smits, and M. Pilon CpSufE Activates the Cysteine Desulfurase CpNifS for Chloroplastic Fe-S Cluster Formation J. Biol. Chem., March 31, 2006; 281(13): 8958 - 8969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shigi, T. Suzuki, T. Terada, M. Shirouzu, S. Yokoyama, and K. Watanabe Temperature-dependent Biosynthesis of 2-Thioribothymidine of Thermus thermophilus tRNA J. Biol. Chem., January 27, 2006; 281(4): 2104 - 2113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Masse, C. K. Vanderpool, and S. Gottesman Effect of RyhB Small RNA on Global Iron Use in Escherichia coli J. Bacteriol., October 15, 2005; 187(20): 6962 - 6971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Huet, M. Daffe, and I. Saves Identification of the Mycobacterium tuberculosis SUF Machinery as the Exclusive Mycobacterial System of [Fe-S] Cluster Assembly: Evidence for Its Implication in the Pathogen's Survival J. Bacteriol., September 1, 2005; 187(17): 6137 - 6146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Loiseau, S. Ollagnier-de Choudens, D. Lascoux, E. Forest, M. Fontecave, and F. Barras Analysis of the Heteromeric CsdA-CsdE Cysteine Desulfurase, Assisting Fe-S Cluster Biogenesis in Escherichia coli J. Biol. Chem., July 22, 2005; 280(29): 26760 - 26769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bruscella, L. Cassagnaud, J. Ratouchniak, G. Brasseur, E. Lojou, R. Amils, and V. Bonnefoy The HiPIP from the acidophilic Acidithiobacillus ferrooxidans is correctly processed and translocated in Escherichia coli, in spite of the periplasm pH difference between these two micro-organisms Microbiology, May 1, 2005; 151(5): 1421 - 1431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Abdel-Ghany, H. Ye, G. F. Garifullina, L. Zhang, E. A.H. Pilon-Smits, and M. Pilon Iron-Sulfur Cluster Biogenesis in Chloroplasts. Involvement of the Scaffold Protein CpIscA Plant Physiology, May 1, 2005; 138(1): 161 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Iwasaki, A. Kounosu, Y. Tao, Z. Li, J. E. Shokes, N. J. Cosper, T. Imai, A. Urushiyama, and R. A. Scott Rational Design of a Mononuclear Metal Site into the Archaeal Rieske-type Protein Scaffold J. Biol. Chem., March 11, 2005; 280(10): 9129 - 9134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. M. Xu, S. Adams, N.-H. Chua, and S. G. Moller AtNAP1 Represents an Atypical SufB Protein in Arabidopsis Plastids J. Biol. Chem., February 25, 2005; 280(8): 6648 - 6654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Djaman, F. W. Outten, and J. A. Imlay Repair of Oxidized Iron-Sulfur Clusters in Escherichia coli J. Biol. Chem., October 22, 2004; 279(43): 44590 - 44599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Pysz, S. B. Conners, C. I. Montero, K. R. Shockley, M. R. Johnson, D. E. Ward, and R. M. Kelly Transcriptional Analysis of Biofilm Formation Processes in the Anaerobic, Hyperthermophilic Bacterium Thermotoga maritima Appl. Envir. Microbiol., October 1, 2004; 70(10): 6098 - 6112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ding, R. J. Clark, and B. Ding IscA Mediates Iron Delivery for Assembly of Iron-Sulfur Clusters in IscU under the Limited Accessible Free Iron Conditions J. Biol. Chem., September 3, 2004; 279(36): 37499 - 37504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Muhlenhoff, J. Balk, N. Richhardt, J. T. Kaiser, K. Sipos, G. Kispal, and R. Lill Functional Characterization of the Eukaryotic Cysteine Desulfurase Nfs1p from Saccharomyces cerevisiae J. Biol. Chem., August 27, 2004; 279(35): 36906 - 36915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hanzelmann, H. L. Hernandez, C. Menzel, R. Garcia-Serres, B. H. Huynh, M. K. Johnson, R. R. Mendel, and H. Schindelin Characterization of MOCS1A, an Oxygen-sensitive Iron-Sulfur Protein Involved in Human Molybdenum Cofactor Biosynthesis J. Biol. Chem., August 13, 2004; 279(33): 34721 - 34732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Tokumoto, S. Kitamura, K. Fukuyama, and Y. Takahashi Interchangeability and Distinct Properties of Bacterial Fe-S Cluster Assembly Systems: Functional Replacement of the isc and suf Operons in Escherichia coli with the nifSU-Like Operon from Helicobacter pylori J. Biochem., August 1, 2004; 136(2): 199 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sutak, P. Dolezal, H. L. Fiumera, I. Hrdy, A. Dancis, M. Delgadillo-Correa, P. J. Johnson, M. Muller, and J. Tachezy From the Cover: Mitochondrial-type assembly of FeS centers in the hydrogenosomes of the amitochondriate eukaryote Trichomonas vaginalis PNAS, July 13, 2004; 101(28): 10368 - 10373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Dutkiewicz, B. Schilke, S. Cheng, H. Knieszner, E. A. Craig, and J. Marszalek Sequence-specific Interaction between Mitochondrial Fe-S Scaffold Protein Isu and Hsp70 Ssq1 Is Essential for Their in Vivo Function J. Biol. Chem., July 9, 2004; 279(28): 29167 - 29174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. M. Xu and S. G. Moller AtNAP7 is a plastidic SufC-like ATP-binding cassette/ATPase essential for Arabidopsis embryogenesis PNAS, June 15, 2004; 101(24): 9143 - 9148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gerber, K. Neumann, C. Prohl, U. Muhlenhoff, and R. Lill The Yeast Scaffold Proteins Isu1p and Isu2p Are Required inside Mitochondria for Maturation of Cytosolic Fe/S Proteins Mol. Cell. Biol., June 1, 2004; 24(11): 4848 - 4857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Lauhon, E. Skovran, H. D. Urbina, D. M. Downs, and L. E. Vickery Substitutions in an Active Site Loop of Escherichia coli IscS Result in Specific Defects in Fe-S Cluster and Thionucleoside Biosynthesis in Vivo J. Biol. Chem., May 7, 2004; 279(19): 19551 - 19558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Dos Santos, A. D. Smith, J. Frazzon, V. L. Cash, M. K. Johnson, and D. R. Dean Iron-Sulfur Cluster Assembly: NifU-DIRECTED ACTIVATION OF THE NITROGENASE Fe PROTEIN J. Biol. Chem., May 7, 2004; 279(19): 19705 - 19711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Ali, Y. Shigeta, U. Tokumoto, Y. Takahashi, and T. Nozaki An Intestinal Parasitic Protist, Entamoeba histolytica, Possesses a Non-redundant Nitrogen Fixation-like System for Iron-Sulfur Cluster Assembly under Anaerobic Conditions J. Biol. Chem., April 16, 2004; 279(16): 16863 - 16874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yabe, K. Morimoto, S. Kikuchi, K. Nishio, I. Terashima, and M. Nakai The Arabidopsis Chloroplastic NifU-Like Protein CnfU, Which Can Act as an Iron-Sulfur Cluster Scaffold Protein, Is Required for Biogenesis of Ferredoxin and Photosystem I PLANT CELL, April 1, 2004; 16(4): 993 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kobayashi, T. Ishizuka, M. Katayama, M. Kanehisa, M. Bhattacharyya-Pakrasi, H. B. Pakrasi, and M. Ikeuchi Response to Oxidative Stress Involves a Novel Peroxiredoxin Gene in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803 Plant Cell Physiol., March 15, 2004; 45(3): 290 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wang, G. Shen, R. Balasubramanian, L. McIntosh, D. A. Bryant, and J. H. Golbeck The sufR Gene (sll0088 in Synechocystis sp. Strain PCC 6803) Functions as a Repressor of the sufBCDS Operon in Iron-Sulfur Cluster Biogenesis in Cyanobacteria J. Bacteriol., February 15, 2004; 186(4): 956 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. W. Outten, M. J. Wood, F. M. Munoz, and G. Storz The SufE Protein and the SufBCD Complex Enhance SufS Cysteine Desulfurase Activity as Part of a Sulfur Transfer Pathway for Fe-S Cluster Assembly in Escherichia coli J. Biol. Chem., November 14, 2003; 278(46): 45713 - 45719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Loiseau, S. Ollagnier-de-Choudens, L. Nachin, M. Fontecave, and F. Barras Biogenesis of Fe-S Cluster by the Bacterial Suf System: SufS AND SufE FORM A NEW TYPE OF CYSTEINE DESULFURASE J. Biol. Chem., October 3, 2003; 278(40): 38352 - 38359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Tantalean, M. A. Araya, C. P. Saavedra, D. E. Fuentes, J. M. Perez, I. L. Calderon, P. Youderian, and C. C. Vasquez The Geobacillus stearothermophilus V iscS Gene, Encoding Cysteine Desulfurase, Confers Resistance to Potassium Tellurite in Escherichia coli K-12 J. Bacteriol., October 1, 2003; 185(19): 5831 - 5837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ollagnier-de Choudens, L. Nachin, Y. Sanakis, L. Loiseau, F. Barras, and M. Fontecave SufA from Erwinia chrysanthemi. CHARACTERIZATION OF A SCAFFOLD PROTEIN REQUIRED FOR IRON-SULFUR CLUSTER ASSEMBLY J. Biol. Chem., May 9, 2003; 278(20): 17993 - 18001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Lauhon Requirement for IscS in Biosynthesis of All Thionucleosides in Escherichia coli J. Bacteriol., December 15, 2002; 184(24): 6820 - 6829. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
