| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 32, 28592-28600, August 9, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Laboratoire de Biologie du Développement, Institut
de la Recherche Agronomique/Unité Mixte de
Recherche-CNRS 7622, Université Pierre et Marie Curie,
Boîte 24, 4 Place Jussieu, 75252 Paris cedex 05, France
Fully grown Xenopus oocyte is
arrested at prophase I of meiosis. Re-entry into meiosis depends on the
activation of MPF (M-phase promoting factor or cyclin B·Cdc2
complex), triggered by progesterone. The prophase-arrested
oocyte contains a store of Cdc2. Most of the protein is present as a
monomer whereas a minor fraction, called pre-MPF, is found to be
associated with cyclin B. Activation of Cdc2 depends on two key events:
cyclin binding and an activating phosphorylation on Thr-161
residue located in the T-loop. To get new insights into the regulation
of Thr-161 phosphorylation of Cdc2, monomeric Cdc2 was isolated from
prophase oocytes. Based on its activation upon cyclin addition and
detection by an antibody directed specifically against Cdc2
phosphorylated on Thr-161, we show for the first time that the prophase
oocyte contains a significant amount of monomeric Cdc2 phosphorylated
on Thr-161. PP2C, a Mg2+-dependent phosphatase,
negatively controls Thr-161 phosphorylation of Cdc2. The unexpected
presence of a population of free Cdc2 already phosphorylated on Thr-161
could contribute to the generation of the Cdc2 kinase activity
threshold required to initiate MPF amplification.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AJ438209.
Thr-161 Phosphorylation of Monomeric Cdc2
REGULATION BY PROTEIN PHOSPHATASE 2C IN XENOPUS
OOCYTES*
,,
*
This research was supported by grants from Institut de la
Recherche Agronomique, CNRS, University Pierre and Marie Curie, ARC (number 5899) and NATO (SfP-972461).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Both authors contributed equally to this work.
§
To whom correspondence should be addressed. Tel.: 33-1-44-27-26-42;
Fax: 33-1-44-27-34-72; E-mail: jessus@ccr.jussieu.fr.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C.-G. Liang, Y.-Q. Su, H.-Y. Fan, H. Schatten, and Q.-Y. Sun Mechanisms Regulating Oocyte Meiotic Resumption: Roles of Mitogen-Activated Protein Kinase Mol. Endocrinol., September 1, 2007; 21(9): 2037 - 2055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Maton, T. Lorca, J.-A. Girault, R. Ozon, and C. Jessus Differential regulation of Cdc2 and Aurora-A in Xenopus oocytes: a crucial role of phosphatase 2A J. Cell Sci., June 1, 2005; 118(11): 2485 - 2494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Karaiskou, A.-C. Lepretre, G. Pahlavan, D. Du Pasquier, R. Ozon, and C. Jessus Polo-like kinase confers MPF autoamplification competence to growing Xenopus oocytes Development, April 1, 2004; 131(7): 1543 - 1552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Maton, C. Thibier, A. Castro, T. Lorca, C. Prigent, and C. Jessus Cdc2-Cyclin B Triggers H3 Kinase Activation of Aurora-A in Xenopus Oocytes J. Biol. Chem., June 6, 2003; 278(24): 21439 - 21449. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
