| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 32, 28601-28608, August 9, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
,,,
, and**
From the Membrane-associated prostaglandin (PG)
E2 synthase (mPGES) is an inducible terminal enzyme
in the biosynthetic pathway for prostaglandin E2, which
participates in many biological processes. In this study, we
investigated the molecular mechanism controlling the inducible
expression of mPGES. The mouse mPGES gene consisted of
three exons, and its 5'-proximal promoter contained consensus motifs
for the binding of several transcription factors. Transgenic expression
in mice of the mouse mPGES promoter flanked by a reporter gene resulted in stimulus-dependent induction of the
reporter in tissues where mPGES was intrinsically induced. Deletion and site-specific mutation analyses of the 5'-flanking region demonstrated that stimulus-inducible expression of mouse and human mPGES required tandem GC boxes adjacent to the initiation site. The stimulus-induced GC box binding activity was present in nuclear extracts of cells, in
which the proximal GC box was essential for binding. An 80-kDa stimulus-inducible nuclear protein that bound to this GC box was identified as the transcription factor Egr-1 (for early
growth response-1). These results suggest that
Egr-1 is a key transcription factor in regulating the inducible
expression of mPGES.
Department of Pharmacology, National
Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai,
Suita, Osaka 565-8565, Japan, the § Department of Health
Chemistry, School of Pharmaceutical Sciences, Showa University,
1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan, and the
¶ Department of Pharmacology, School of Pharmaceutical Sciences,
Kitasato University, Shirokane 5-9-1, Minato-ku,
Tokyo 108-8641, Japan
The nucleotide sequence(s) reported in this paper has been submitted to the DDBJ/GenBankTM/EBI Data Bank with accession number(s) AB083340.
Present address: Kitasato Institute, Tokyo 108-8642, Japan.
**
To whom correspondence should be addressed. Tel.: 81-6-6833-5012;
Fax: 81-6-6872-8090; E-mail: tanabe@ri.ncvc.go.jp.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Kojima, M. Kapoor, L. Yang, E. L. Fleishaker, M. R. Ward, S. U. Monrad, P. C. Kottangada, C. Q. Pace, J. A. Clark, J. G. Woodward, et al. Defective Generation of a Humoral Immune Response Is Associated with a Reduced Incidence and Severity of Collagen-Induced Arthritis in Microsomal Prostaglandin E Synthase-1 Null Mice J. Immunol., June 15, 2008; 180(12): 8361 - 8368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. F. Rodriguez, L. A. Blomberg, K. A. Zuelke, J. R. Miles, J. E. Alexander, and C. E. Farin Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression Physiol Genomics, November 21, 2006; 27(3): 318 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sayasith, K. A Brown, J. G Lussier, M. Dore, and J. Sirois Characterization of bovine early growth response factor-1 and its gonadotropin-dependent regulation in ovarian follicles prior to ovulation. J. Mol. Endocrinol., October 1, 2006; 37(2): 239 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-W. Wang, C.-T. Hsueh, C.-F. J. Lin, T.-Y. Chou, W.-H. Hsu, L.-S. Wang, and Y.-C. Wu Clinical Implications of Microsomal Prostaglandin E Synthase-1 Overexpression in Human Non-Small-Cell Lung Cancer Ann. Surg. Oncol., September 1, 2006; 13(9): 1224 - 1234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Inada, C. Matsumoto, S. Uematsu, S. Akira, and C. Miyaura Membrane-Bound Prostaglandin E Synthase-1-Mediated Prostaglandin E2 Production by Osteoblast Plays a Critical Role in Lipopolysaccharide-Induced Bone Loss Associated with Inflammation J. Immunol., August 1, 2006; 177(3): 1879 - 1885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Degousee, D. Angoulvant, S. Fazel, E. Stefanski, S. Saha, K. Iliescu, T. F. Lindsay, J. E. Fish, P. A. Marsden, R.-K. Li, et al. c-Jun N-terminal Kinase-mediated Stabilization of Microsomal Prostaglandin E2 Synthase-1 mRNA Regulates Delayed Microsomal Prostaglandin E2 Synthase-1 Expression and Prostaglandin E2 Biosynthesis by Cardiomyocytes J. Biol. Chem., June 16, 2006; 281(24): 16443 - 16452. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fujino and J. W. Regan EP4 Prostanoid Receptor Coupling to a Pertussis Toxin-Sensitive Inhibitory G Protein Mol. Pharmacol., January 1, 2006; 69(1): 5 - 10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Moon, W. C. Glasgow, and T. E. Eling Curcumin Suppresses Interleukin 1{beta}-Mediated Microsomal Prostaglandin E Synthase 1 by Altering Early Growth Response Gene 1 and Other Signaling Pathways J. Pharmacol. Exp. Ther., November 1, 2005; 315(2): 788 - 795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Parent and M. A. Fortier Expression and Contribution of Three Different Isoforms of Prostaglandin E Synthase in the Bovine Endometrium Biol Reprod, July 1, 2005; 73(1): 36 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. H. Faour, N. Alaaeddine, A. Mancini, Q. W. He, D. Jovanovic, and J. A. Di Battista Early Growth Response Factor-1 Mediates Prostaglandin E2-dependent Transcriptional Suppression of Cytokine-induced Tumor Necrosis Factor-{alpha} Gene Expression in Human Macrophages and Rheumatoid Arthritis-affected Synovial Fibroblasts J. Biol. Chem., March 11, 2005; 280(10): 9536 - 9546. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Pini, T. Grosser, J. A. Lawson, T. S. Price, M. A. Pack, and G. A. FitzGerald Prostaglandin E Synthases in Zebrafish Arterioscler. Thromb. Vasc. Biol., February 1, 2005; 25(2): 315 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shinji, T. Tsukui, A. Tatsuguchi, K. Shinoki, M. Kusunoki, K. Suzuki, T. Hiratsuka, K. Wada, S. Futagami, K. Miyake, et al. Induced microsomal PGE synthase-1 is involved in cyclooxygenase-2-dependent PGE2 production in gastric fibroblasts Am J Physiol Gastrointest Liver Physiol, February 1, 2005; 288(2): G308 - G315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Giannico, M. Mendez, and M. C. LaPointe Regulation of the membrane-localized prostaglandin E synthases mPGES-1 and mPGES-2 in cardiac myocytes and fibroblasts Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H165 - H174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kamei, K. Yamakawa, Y. Takegoshi, M. Mikami-Nakanishi, Y. Nakatani, S. Oh-ishi, H. Yasui, Y. Azuma, N. Hirasawa, K. Ohuchi, et al. Reduced Pain Hypersensitivity and Inflammation in Mice Lacking Microsomal Prostaglandin E Synthase-1 J. Biol. Chem., August 6, 2004; 279(32): 33684 - 33695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cheng, H. Afif, J. Martel-Pelletier, J.-P. Pelletier, X. Li, K. Farrajota, M. Lavigne, and H. Fahmi Activation of Peroxisome Proliferator-activated Receptor {gamma} Inhibits Interleukin-1{beta}-induced Membrane-associated Prostaglandin E2 Synthase-1 Expression in Human Synovial Fibroblasts by Interfering with Egr-1 J. Biol. Chem., May 21, 2004; 279(21): 22057 - 22065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Subbaramaiah, K. Yoshimatsu, E. Scherl, K. M. Das, K. D. Glazier, D. Golijanin, R. A. Soslow, T. Tanabe, H. Naraba, and A. J. Dannenberg Microsomal Prostaglandin E Synthase-1 Is Overexpressed in Inflammatory Bowel Disease: EVIDENCE FOR INVOLVEMENT OF THE TRANSCRIPTION FACTOR Egr-1 J. Biol. Chem., March 26, 2004; 279(13): 12647 - 12658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Golijanin, J.-Y. Tan, A. Kazior, E. G. Cohen, P. Russo, G. Dalbagni, K. J. Auborn, K. Subbaramaiah, and A. J. Dannenberg Cyclooxygenase-2 and Microsomal Prostaglandin E Synthase-1 Are Overexpressed in Squamous Cell Carcinoma of the Penis Clin. Cancer Res., February 1, 2004; 10(3): 1024 - 1031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mendez and M. C. LaPointe PPAR{gamma} Inhibition of Cyclooxygenase-2, PGE2 Synthase, and Inducible Nitric Oxide Synthase in Cardiac Myocytes Hypertension, October 1, 2003; 42(4): 844 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Murakami, K. Nakashima, D. Kamei, S. Masuda, Y. Ishikawa, T. Ishii, Y. Ohmiya, K. Watanabe, and I. Kudo Cellular Prostaglandin E2 Production by Membrane-bound Prostaglandin E Synthase-2 via Both Cyclooxygenases-1 and -2 J. Biol. Chem., September 26, 2003; 278(39): 37937 - 37947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Liu, Z. Zhuang, P. W. Hoffman, and G. Bai Functional Analysis of the Rat N-Methyl-D-aspartate Receptor 2A Promoter: MULTIPLE TRANSCRIPTION START POINTS, POSITIVE REGULATION BY Sp FACTORS, AND TRANSLATIONAL REGULATION J. Biol. Chem., July 11, 2003; 278(29): 26423 - 26434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kamei, M. Murakami, Y. Nakatani, Y. Ishikawa, T. Ishii, and I. Kudo Potential Role of Microsomal Prostaglandin E Synthase-1 in Tumorigenesis J. Biol. Chem., May 23, 2003; 278(21): 19396 - 19405. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Russell, K. M. H. Doyle, I. Gonzales-Robayna, C. Pipaon, and J. S. Richards Egr-1 Induction in Rat Granulosa Cells by Follicle-Stimulating Hormone and Luteinizing Hormone: Combinatorial Regulation By Transcription Factors Cyclic Adenosine 3',5'-Monophosphate Regulatory Element Binding Protein, Serum Response Factor, Sp1, and Early Growth Response Factor-1 Mol. Endocrinol., April 1, 2003; 17(4): 520 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fujino, W. Xu, and J. W. Regan Prostaglandin E2 Induced Functional Expression of Early Growth Response Factor-1 by EP4, but Not EP2, Prostanoid Receptors via the Phosphatidylinositol 3-Kinase and Extracellular Signal-regulated Kinases J. Biol. Chem., March 28, 2003; 278(14): 12151 - 12156. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
