HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 32, 28714-28724, August 9, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/32/28714    most recent M111935200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Barat, C. Articles by Tremblay, M. J. Search for Related Content PubMed PubMed Citation Articles by Barat, C. Articles by Tremblay, M. J. Social Bookmarking                      What's this?

Engagement of CD43 Enhances Human Immunodeficiency Virus Type 1 Transcriptional Activity and Virus Production That Is Induced upon TCR/CD3 Stimulation^*

Corinne Barat and Michel J. Tremblay^§

From the  Centre de Recherche en Infectiologie, Hôpital CHUL, Centre Hospitalier Universitaire de Québec, and Département de Biologie Médicale, Faculté de Médecine, Université Laval, Ste-Foy, Québec G1V 4G2, Canada

Human immunodeficiency virus type 1 (HIV-1) transcriptional activity is regulated by several cytokines and T cell activators. CD43 (sialophorin) is a sialoglycoprotein expressed on the surface of a wide variety of blood cells including T lymphocytes. Several studies have shown that CD43 ligation induces proliferation and activation of human T lymphocytes. We were thus interested in defining whether CD43-mediated signaling events can modulate the life cycle of HIV-1. We demonstrate here that CD43 cross-linking potentiates HIV-1 promoter-driven activity and virus production that is seen following the engagement of the T-cell receptor (TCR)·CD3 complex. This effect is independent of the CD28 co-stimulatory molecule and is mediated by both NF-B and NFAT transcription factors. A number of signal transducers known to be involved in the TCR/CD3-dependent signal transduction pathway, including p56^lck, p36^lat, and SLP-76, as well as capacitative entry of calcium, are crucial for the noticed CD43 co-stimulatory effect. Calcium mobilization studies indicate that a synergy is occurring between CD43- and TCR/CD3-mediated signaling events leading to an augmented calcium release. These data suggest that CD43 can be seen as a co-stimulatory cell surface constituent that can modulate HIV-1 expression in T lymphocytes.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. Thibault, M. R. Tardif, C. Barat, and M. J. Tremblay TLR2 Signaling Renders Quiescent Naive and Memory CD4+ T Cells More Susceptible to Productive Infection with X4 and R5 HIV-Type 1 J. Immunol., October 1, 2007; 179(7): 4357 - 4366. [Abstract] [Full Text] [PDF]

				N. A. Fierro, G. Pedraza-Alva, and Y. Rosenstein TCR-Dependent Cell Response Is Modulated by the Timing of CD43 Engagement. J. Immunol., June 15, 2006; 176(12): 7346 - 7353. [Abstract] [Full Text] [PDF]

				M. R. Tardif and M. J. Tremblay Tetraspanin CD81 Provides a Costimulatory Signal Resulting in Increased Human Immunodeficiency Virus Type 1 Gene Expression in Primary CD4+ T Lymphocytes through NF-{kappa}B, NFAT, and AP-1 Transduction Pathways J. Virol., April 1, 2005; 79(7): 4316 - 4328. [Abstract] [Full Text] [PDF]

				I. Mattioli, O. Dittrich-Breiholz, M. Livingstone, M. Kracht, and M. L. Schmitz Comparative analysis of T-cell costimulation and CD43 activation reveals novel signaling pathways and target genes Blood, November 15, 2004; 104(10): 3302 - 3304. [Abstract] [Full Text] [PDF]

				C. Barat, P. Gervais, and M. J. Tremblay Engagement of ICAM-3 Provides a Costimulatory Signal for Human Immunodeficiency Virus Type 1 Replication in both Activated and Quiescent CD4+ T Lymphocytes: Implications for Virus Pathogenesis J. Virol., June 15, 2004; 78(12): 6692 - 6697. [Abstract] [Full Text] [PDF]

				E. Layseca-Espinosa, G. Pedraza-Alva, J. L. Montiel, R. del Rio, N. A. Fierro, R. Gonzalez-Amaro, and Y. Rosenstein T cell aggregation induced through CD43: intracellular signals and inhibition by the immunomodulatory drug leflunomide J. Leukoc. Biol., December 1, 2003; 74(6): 1083 - 1093. [Abstract] [Full Text]

				M. E. Cruz-Munoz, E. Salas-Vidal, N. Salaiza-Suazo, I. Becker, G. Pedraza-Alva, and Y. Rosenstein The CD43 Coreceptor Molecule Recruits the {zeta}-Chain as Part of Its Signaling Pathway J. Immunol., August 15, 2003; 171(4): 1901 - 1908. [Abstract] [Full Text] [PDF]