Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/jbc.M203399200 on June 4, 2002

J. Biol. Chem., Vol. 277, Issue 32, 28780-28786, August 9, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/32/28780    most recent
M203399200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morita, D.
Right arrow Articles by Mizuta, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morita, D.
Right arrow Articles by Mizuta, K.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Rpf2p, an Evolutionarily Conserved Protein, Interacts with Ribosomal Protein L11 and Is Essential for the Processing of 27 SB Pre-rRNA to 25 S rRNA and the 60 S Ribosomal Subunit Assembly in Saccharomyces cerevisiae*

Daisuke MoritaDagger , Keita MiyoshiDagger §, Yasushi Matsui||, Akio Toh-e||, Hidenori ShinkawaDagger , Tokichi MiyakawaDagger , and Keiko MizutaDagger §**

From the Dagger  Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter and the § Department of Bioresource Science and Technology, Graduate School of Biosphere Sciences, Hiroshima University, Kagamiyama 1-4-4, Higashi-Hiroshima 739-8528, Japan and the || Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

Saccharomyces cerevisiae Rrs1p is a nuclear protein that is essential for the maturation of 25 S rRNA and the 60 S ribosomal subunit assembly. In two-hybrid screening, using RRS1 as bait, we have cloned YKR081c/RPF2. Rpf2p is essential for growth and is mainly localized in the nucleolus. The amino acid sequence of Rpf2p is highly conserved in eukaryotes from yeast to human. Similar to Rrs1p, Rpf2p shows physical interaction with ribosomal protein L11 and appears to associate with preribosomal subunits fairly tightly. Northern, methionine pulse-chase, and sucrose density gradient ultracentrifugation analyses reveal that the depletion of Rpf2p results in a delayed processing of pre-rRNA, a decrease of mature 25 S rRNA, and a shortage of 60 S subunits. An analysis of processing intermediates by primer extension shows that the Rpf2p depletion leads to an accumulation of 27 SB pre-rRNA, suggesting that Rpf2p is required for the processing of 27 SB into 25 S rRNA.


* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Recipient of a Japan Society for the Promotion of Science predoctoral research fellowship.

** To whom correspondence should be addressed. Tel.: 81-824-24-7926; Fax: 81-824-24-7926; E-mail: kmizuta@hiroshima-u.ac.jp.


Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
GENES CELLSHome page
Y. Hirano, K. Ishii, M. Kumeta, K. Furukawa, K. Takeyasu, and T. Horigome
Proteomic and targeted analytical identification of BXDC1 and EBNA1BP2 as dynamic scaffold proteins in the nucleolus.
Genes Cells, February 1, 2009; 14(2): 155 - 166.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
L. Tang, A. Sahasranaman, J. Jakovljevic, E. Schleifman, and J. L. Woolford Jr.
Interactions among Ytm1, Erb1, and Nop7 Required for Assembly of the Nop7-Subcomplex in Yeast Preribosomes
Mol. Biol. Cell, July 1, 2008; 19(7): 2844 - 2856.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
J. Zhang, P. Harnpicharnchai, J. Jakovljevic, L. Tang, Y. Guo, M. Oeffinger, M. P. Rout, S. L. Hiley, T. Hughes, and J. L. Woolford Jr.
Assembly factors Rpf2 and Rrs1 recruit 5S rRNA and ribosomal proteins rpL5 and rpL11 into nascent ribosomes
Genes & Dev., October 15, 2007; 21(20): 2580 - 2592.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
M. Nariai, T. Tanaka, T. Okada, C. Shirai, C. Horigome, and K. Mizuta
Synergistic defect in 60S ribosomal subunit assembly caused by a mutation of Rrs1p, a ribosomal protein L11-binding protein, and 3'-extension of 5S rRNA in Saccharomyces cerevisiae
Nucleic Acids Res., August 12, 2005; 33(14): 4553 - 4562.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. Shirai, T. Takai, M. Nariai, C. Horigome, and K. Mizuta
Ebp2p, the Yeast Homolog of Epstein-Barr Virus Nuclear Antigen 1-binding Protein 2, Interacts with Factors of Both the 60 S and the 40 S Ribosomal Subunit Assembly
J. Biol. Chem., June 11, 2004; 279(24): 25353 - 25358.
[Abstract] [Full Text] [PDF]


Home page
RNAHome page
E. W. HORSEY, J. JAKOVLJEVIC, T. D. MILES, P. HARNPICHARNCHAI, and J. L. WOOLFORD JR.
Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation
RNA, May 1, 2004; 10(5): 813 - 827.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
K. Miyoshi, C. Shirai, and K. Mizuta
Transcription of genes encoding trans-acting factors required for rRNA maturation/ribosomal subunit assembly is coordinately regulated with ribosomal protein genes and involves Rap1 in Saccharomyces cerevisiae
Nucleic Acids Res., April 1, 2003; 31(7): 1969 - 1973.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement