Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M204381200 on June 4, 2002

J. Biol. Chem., Vol. 277, Issue 33, 29617-29625, August 16, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/33/29617    most recent
M204381200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Strezoska, Z.
Right arrow Articles by Lau, L. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Strezoska, Z.
Right arrow Articles by Lau, L. F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Functional Inactivation of the Mouse Nucleolar Protein Bop1 Inhibits Multiple Steps in Pre-rRNA Processing and Blocks Cell Cycle Progression*

Zaklina Strezoska, Dimitri G. Pestov, and Lester F. LauDagger

From the Department of Molecular Genetics, University of Illinois College of Medicine, Chicago, Illinois 60607-7170

Bop1 is a conserved nucleolar protein involved in rRNA processing and ribosome assembly in eukaryotes. Expression of its dominant-negative mutant Bop1Delta in mouse cells blocks rRNA maturation and synthesis of large ribosomal subunits and induces a reversible, p53-dependent cell cycle arrest. In this study, we have conducted a deletion analysis of Bop1 and identified a new mutant, Bop1N2, that also acts as a potent inhibitor of cell cycle progression. Bop1N2 and Bop1Delta are C-terminal and N-terminal deletion mutants, respectively, and share only 72 amino acid residues. Both mutant proteins are localized to the nucleolus and strongly inhibit rRNA processing, suggesting that activation of a cell cycle checkpoint by Bop1 mutants is linked to their inhibitory effects on rRNA and ribosome synthesis. By using these dominant-negative mutants as well as antisense oligonucleotides to interfere with endogenous Bop1, we identified specific rRNA processing steps that require Bop1 function in mammalian cells. Our data demonstrate that Bop1 is required for proper processing at four distinct sites located within the internal transcribed spacers ITS1 and ITS2 and the 3' external spacer. We propose a model in which Bop1 serves as an essential factor in ribosome formation that coordinates processing of the spacer regions in pre-rRNA.

* This work was supported by National Institutes of Health Grant CA95627.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Molecular Genetics (M/C 669), University of Illinois, 900 South Ashland Ave., Chicago, IL 60607. Tel.: 312-996-6978; Fax: 312-996-7034; E-mail: LFLau@uic.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
L. Tang, A. Sahasranaman, J. Jakovljevic, E. Schleifman, and J. L. Woolford Jr.
Interactions among Ytm1, Erb1, and Nop7 Required for Assembly of the Nop7-Subcomplex in Yeast Preribosomes
Mol. Biol. Cell, July 1, 2008; 19(7): 2844 - 2856.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Shetty, S. H. Shao, and C. C. Y. Weng
p53-Dependent Apoptosis in the Inhibition of Spermatogonial Differentiation in Juvenile Spermatogonial Depletion (Utp14bjsd) Mice
Endocrinology, June 1, 2008; 149(6): 2773 - 2781.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
N. D. Murchison, B. A. Price, D. A. Conner, D. R. Keene, E. N. Olson, C. J. Tabin, and R. Schweitzer
Regulation of tendon differentiation by scleraxis distinguishes force-transmitting tendons from muscle-anchoring tendons
Development, July 15, 2007; 134(14): 2697 - 2708.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. E. Griffith, U. Mayer, A. Capron, Q. A. Ngo, A. Surendrarao, R. McClinton, G. Jurgens, and V. Sundaresan
The TORMOZ Gene Encodes a Nucleolar Protein Required for Regulated Division Planes and Embryo Development in Arabidopsis
PLANT CELL, July 1, 2007; 19(7): 2246 - 2263.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Rohrmoser, M. Holzel, T. Grimm, A. Malamoussi, T. Harasim, M. Orban, I. Pfisterer, A. Gruber-Eber, E. Kremmer, and D. Eick
Interdependence of Pes1, Bop1, and WDR12 Controls Nucleolar Localization and Assembly of the PeBoW Complex Required for Maturation of the 60S Ribosomal Subunit
Mol. Cell. Biol., May 15, 2007; 27(10): 3682 - 3694.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. A. Bernstein, F. Bleichert, J. M. Bean, F. R. Cross, and S. J. Baserga
Ribosome Biogenesis Is Sensed at the Start Cell Cycle Checkpoint
Mol. Biol. Cell, March 1, 2007; 18(3): 953 - 964.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Beekman, M. Nichane, S. De Clercq, M. Maetens, T. Floss, W. Wurst, E. Bellefroid, and J.-C. Marine
Evolutionarily Conserved Role of Nucleostemin: Controlling Proliferation of Stem/Progenitor Cells during Early Vertebrate Development
Mol. Cell. Biol., December 15, 2006; 26(24): 9291 - 9301.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Zhang, R. B. Hamanaka, E. Bobrovnikova-Marjon, J. D. Gordan, M.-S. Dai, H. Lu, M. C. Simon, and J. A. Diehl
Ribosomal Stress Couples the Unfolded Protein Response to p53-dependent Cell Cycle Arrest
J. Biol. Chem., October 6, 2006; 281(40): 30036 - 30045.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Grimm, M. Holzel, M. Rohrmoser, T. Harasim, A. Malamoussi, A. Gruber-Eber, E. Kremmer, and D. Eick
Dominant-negative Pes1 mutants inhibit ribosomal RNA processing and cell proliferation via incorporation into the PeBoW-complex
Nucleic Acids Res., May 31, 2006; 34(10): 3030 - 3043.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. D. Miles, J. Jakovljevic, E. W. Horsey, P. Harnpicharnchai, L. Tang, and J. L. Woolford Jr
Ytm1, Nop7, and Erb1 Form a Complex Necessary for Maturation of Yeast 66S Preribosomes
Mol. Cell. Biol., December 1, 2005; 25(23): 10419 - 10432.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. Holzel, M. Rohrmoser, M. Schlee, T. Grimm, T. Harasim, A. Malamoussi, A. Gruber-Eber, E. Kremmer, W. Hiddemann, G. W. Bornkamm, et al.
Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation
J. Cell Biol., August 1, 2005; 170(3): 367 - 378.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. A. Bernstein and S. J. Baserga
The Small Subunit Processome Is Required for Cell Cycle Progression at G1
Mol. Biol. Cell, November 1, 2004; 15(11): 5038 - 5046.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M.-S. Dai and H. Lu
Inhibition of MDM2-mediated p53 Ubiquitination and Degradation by Ribosomal Protein L5
J. Biol. Chem., October 22, 2004; 279(43): 44475 - 44482.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M.-S. Dai, S. X. Zeng, Y. Jin, X.-X. Sun, L. David, and H. Lu
Ribosomal Protein L23 Activates p53 by Inhibiting MDM2 Function in Response to Ribosomal Perturbation but Not to Translation Inhibition
Mol. Cell. Biol., September 1, 2004; 24(17): 7654 - 7668.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Henning, R. B. So, R. Jin, L. F. Lau, and B. C. Valdez
Silencing of RNA Helicase II/Gu{alpha} Inhibits Mammalian Ribosomal RNA Production
J. Biol. Chem., December 26, 2003; 278(52): 52307 - 52314.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Yang, J. Zhou, R. L. Ochs, D. Henning, R. Jin, and B. C. Valdez
Down-regulation of RNA Helicase II/Gu Results in the Depletion of 18 and 28 S rRNAs in Xenopus Oocyte
J. Biol. Chem., October 3, 2003; 278(40): 38847 - 38859.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. H. Geerlings, A. W. Faber, M. D. Bister, J. C. Vos, and H. A. Raue
Rio2p, an Evolutionarily Conserved, Low Abundant Protein Kinase Essential for Processing of 20 S Pre-rRNA in Saccharomyces cerevisiae
J. Biol. Chem., June 13, 2003; 278(25): 22537 - 22545.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Lerch-Gaggl, J. Haque, J. Li, G. Ning, P. Traktman, and S. A. Duncan
Pescadillo Is Essential for Nucleolar Assembly, Ribosome Biogenesis, and Mammalian Cell Proliferation
J. Biol. Chem., November 15, 2002; 277(47): 45347 - 45355.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement