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Originally published In Press as doi:10.1074/jbc.M202435200 on June 7, 2002

J. Biol. Chem., Vol. 277, Issue 33, 29730-29736, August 16, 2002
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Syndecan-2 Mediates Adhesion and Proliferation of Colon Carcinoma Cells*

Haein ParkDagger §, Yeonhee KimDagger , Yangmi LimDagger §, Innoc Han, and Eok-Soo OhDagger ||

From the Dagger  Department of Life Sciences, Division of Molecular Life Sciences and Center for Cell Signaling Research and  Ewha Institute of Neuroscience, Ewha Medical School, Ewha Women's University, Seoul 120-750 Korea

Syndecan-2 is a transmembrane heparan sulfate proteoglycan whose function at the cell surface is unclear. In this study, we examined the function of syndecan-2 in colon cancer cell lines. In several colon cancer cell lines, syndecan-2 was highly expressed compared with normal cell lines. In contrast, syndecan-1 and -4 were decreased. Cell biological studies using the extracellular domain of recombinant syndecan-2 (2E) or spreading assay with syndecan-2 antibody-coated plates showed that syndecan-2 mediated adhesion and cytoskeletal organization of colon cancer cells. This interaction was critical for the proliferation of colon carcinoma cells. Blocking with 2E or antisense syndecan-2 cDNA induced G0/G1 cell cycle arrest with concomitantly increased expression of p21, p27, and p53. Furthermore, blocking of syndecan-2 through antisense syndecan-2 cDNA significantly reduced tumorigenic activity in colon carcinoma cells. Therefore, increased syndecan-2 expression appears to be a critical for colon carcinoma cell behavior, and syndecan-2 regulates tumorigenic activity through regulation of adhesion and proliferation in colon carcinoma cells.

* This work was supported by Ministry of Health and Welfare Research Grant HMP-00-B-20800-0080) and Korea Science and Engineering Foundation (KOSEF) through the Center for Cell Signaling Research at Ewha Women's University.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Supported by a fellowship from Brain Korea 21 project.

|| To whom correspondence should be addressed: Center for Cell Signaling Research, Ewha Women's University, Daehyun-dong, Seodaemoon-Gu, Seoul 120-750 Korea. Tel.: 82-2-3277-3761; Fax: 82-2-3277-3760; E-mail: OhES@mm.ewha.ac.kr.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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