| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 33, 30055-30065, August 16, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Departments of RFLAT-1/KLF13, a member of the
Krüppel-like family of transcription factors, was identified as a
transcription factor expressed 3-5 days after T lymphocyte activation.
It binds to the promoter of the chemokine gene RANTES (regulated on
activation normal T cell expressed and secreted) and regulates its
"late" expression in activated T-cells. In this study, a series
of experiments to define the functional domains of RFLAT-1/KLF13 were
undertaken to further advance the understanding of the molecular
mechanisms underlying transcriptional regulation by this factor. Using
the GAL4 fusion system, distinct transcriptional activation and
repression domains were identified. The RFLAT-1 minimum activation
domain is localized to amino acids 1-35, whereas the repression domain resides in amino acids 67-168. Deletion analysis on the RFLAT-1 protein further supports these domain functions. The RFLAT-1 activation domain is similar to that of its closest family member, basic transcription element-binding protein 1. This domain is highly hydrophobic, and site-directed mutagenesis demonstrated that both negatively charged and hydrophobic residues are important for transactivation. The nuclear localization signal of RFLAT-1 was also
identified using the RFLAT-1/green fluorescence protein fusion approach. RFLAT-1 contains two potent, independent nuclear localization signals; one is immediately upstream of the zinc finger DNA-binding domain, and the other is within the zinc fingers. Using mutational analysis, we also determined that the critical binding sequence of
RFLAT-1 is CTCCC. The intact CTCCC box on the RANTES
promoter is necessary for RFLAT-1-mediated RANTES
transcription and is also required for the synergy between RFLAT-1 and
NF-
Functional Domains and DNA-binding Sequences of
RFLAT-1/KLF13, a Krüppel-like Transcription Factor of
Activated T Lymphocytes*
§,¶,
,,,**, and
Pediatrics and
** Cardiothoracic Surgery, Stanford University School of
Medicine, Stanford, California 94305-5164
B proteins.
*
This work was supported by Grant DK35008 from the National
Institutes of Health (to A. M. K.), by Grant PF-77419 from
the Elizabeth Glaser Pediatric AIDS Foundation (to A. S.), and by the Satellite Dialysis Young Investigator Grant of the National Kidney
Foundation (to A. S.).The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Scripps Institution of Oceanography,
University of California, San Diego, La Jolla, CA 92093-0202.
Shelagh Galligan Professor of Pediatrics.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Ohguchi, T. Tanaka, A. Uchida, K. Magoori, H. Kudo, I. Kim, K. Daigo, I. Sakakibara, M. Okamura, H. Harigae, et al. Hepatocyte Nuclear Factor 4{alpha} Contributes to Thyroid Hormone Homeostasis by Cooperatively Regulating the Type 1 Iodothyronine Deiodinase Gene with GATA4 and Kruppel-Like Transcription Factor 9 Mol. Cell. Biol., June 15, 2008; 28(12): 3917 - 3931. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Huang, Y.-T. Ahn, L. McPherson, C. Clayberger, and A. M. Krensky Interaction of PRP4 with Kruppel-Like Factor 13 Regulates CCL5 Transcription J. Immunol., June 1, 2007; 178(11): 7081 - 7087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhou, L. McPherson, D. Feng, A. Song, C. Dong, S.-C. Lyu, L. Zhou, X. Shi, Y.-T. Ahn, D. Wang, et al. Kruppel-Like Transcription Factor 13 Regulates T Lymphocyte Survival In Vivo J. Immunol., May 1, 2007; 178(9): 5496 - 5504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-T. Ahn, B. Huang, L. McPherson, C. Clayberger, and A. M. Krensky Dynamic Interplay of Transcriptional Machinery and Chromatin Regulates "Late" Expression of the Chemokine RANTES in T Lymphocytes Mol. Cell. Biol., January 1, 2007; 27(1): 253 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tabrizifard, A. Olaru, J. Plotkin, M. Fallahi-Sichani, F. Livak, and H. T. Petrie Analysis of Transcription Factor Expression during Discrete Stages of Postnatal Thymocyte Differentiation J. Immunol., July 15, 2004; 173(2): 1094 - 1102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Woodward, Y. Wang, W. J. Dixon, H. Htun, and S. A. Chow Subcellular Localization of Feline Immunodeficiency Virus Integrase and Mapping of Its Karyophilic Determinant J. Virol., April 15, 2003; 77(8): 4516 - 4527. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
