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Originally published In Press as doi:10.1074/jbc.M201167200 on June 12, 2002

J. Biol. Chem., Vol. 277, Issue 34, 30488-30494, August 23, 2002
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trans-Lesion Synthesis Past Bulky Benzo[a]pyrene Diol Epoxide N2-dG and N6-dA Lesions Catalyzed by DNA Bypass Polymerases*

Olga RechkoblitDagger , Yanbin Zhang§, Dongyu Guo§, Zhigang Wang§, Shantu Amin, Jacek Krzeminsky, Natalia LounevaDagger , and Nicholas E. GeacintovDagger ||

From the Dagger  Chemistry Department, New York University, New York, New York 10003-5180, § Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky 40536, and  American Health Foundation, Valhalla, New York 10595

The effectiveness of in vitro primer elongation reactions catalyzed by human bypass DNA polymerases kappa  (hDinB1), pol eta  (hRad30A), pol iota  (hRad30B), and yeast pol zeta  (Rev3 and Rev7) in site-specifically modified template oligonucleotide strands were studied in vitro. The templates contained single bulky lesions derived from the trans-addition of the mutagenic (+)- or (-)-enantiomers of r7,t8-dihydroxy-t9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (a metabolite of the environmental carcinogen benzo[a]pyrene), to the exocyclic amino groups of guanine or adenine in oligonucleotide templates 33, or more, bases long. In "running start" primer extension reactions, pol kappa  effectively bypassed both the stereoisomeric (+)- and (-)-trans-guanine adducts but not the analogous adenine adducts. In sharp contrast, pol eta , which exhibits considerable sequence homology with pol kappa  (both belong to the group of Y family polymerases), is partially blocked by the guanine adducts and the (-)-trans-adenine adduct, although the stereoisomeric (+)-trans-adenine adduct is more successfully bypassed. Neither pol iota  nor pol zeta , either alone or in combination, were effective in trans-lesion synthesis past the same adducts. In all cases, the fidelity of insertion is dependent on adduct stereochemistry and structure. Generally, error-free nucleotide insertion opposite the lesions tends to depend more on adduct stereochemistry than error-prone insertion. None of the polymerases tested are a universal bypass polymerase for the stereoisomeric bulky polycyclic aromatic hydrocarbon-DNA adducts derived from anti-BPDE.

* This work was supported by National Institutes of Health Grants CA20851 (to N. E. G.) and CA92768 (to Z. W.) and the New Investigator award in Toxicology from the Burrough Wellcome Fund.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed: Chemistry Dept., New York University, 29 Washington Place, New York, NY 10003. Tel.: 212-998-8407; Fax: 212-998-8421; E-mail: nicholas.geacintov@nyu.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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