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J. Biol. Chem., Vol. 277, Issue 34, 30598-30605, August 23, 2002
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From Microbial Collection and Screening Laboratory, the
Institute of Molecular and Cell Biology, Singapore 117609
Siderophores are small organic compounds with
high affinity for ferric iron. Microorganisms commonly acquire iron via
siderophore secretion and uptake. Here we report the characterization
of the siderophore transporter CaArn1p in the fungal pathogen
Candida albicans. Deletion of CaARN1 reduced
the ability of C. albicans to use iron bound to the
hydroxamate-type siderophore ferrichrome and abolished it when two
high-affinity iron permease genes (CaFTR1 and
CaFTR2) were also deleted, indicating a role of CaArn1p as well as the permeases in ferrichrome-iron uptake. Caarn1
Characterization and Functional Analysis of the Siderophore-Iron
Transporter CaArn1p in Candida albicans*
(but not Caftr1Caftr2) assimilated iron from another
hydroxamate-type siderophore, ferrioxamine B, suggesting that iron
uptake from this compound depends on the permeases, but not on CaArn1p.
Northern blot analysis revealed that the transcription repressor
CaTup1p repressed CaARN1 expression under iron-replete
conditions via the DNA-binding protein Rfg1p. Green fluorescent
protein-tagged CaArn1p was observed predominantly in the plasma
membrane, with some in the cytoplasm as distinct spots. The number of
these spots increased with the increase in ferrichrome concentration,
suggesting that CaArn1p internalization might be a mechanism for
ferrichrome-iron uptake or for recycling the transporter.
Caarn1 did not show reduced virulence when injected into
the blood stream of mice, implying that CaArn1p is not required for
iron uptake along this route of infection.
*
This work was supported by the Agency for Science,
Technology, and Research of Singapore.The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Adjunct Associate Professor in the Department of Microbiology,
National University of Singapore. To whom correspondence should be
addressed: Inst. of Molecular and Cell Biology, 30 Medical Dr.,
Singapore 117609. Tel.: 65-6778-3207; Fax: 65-6779-1117; E-mail:
mcbwangy@imcb.nus.edu.sg.
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