HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 277, Issue 34, 30844-30851, August 23, 2002

This Article Full Text Full Text (PDF) All Versions of this Article: 277/34/30844    most recent M201877200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shuen, M. Articles by Mymryk, J. S. Search for Related Content PubMed PubMed Citation Articles by Shuen, M. Articles by Mymryk, J. S. Social Bookmarking                      What's this?

The Adenovirus E1A Protein Targets the SAGA but Not the ADA Transcriptional Regulatory Complex through Multiple Independent Domains^*

Michael Shuen^§¶, Nikita Avvakumov, Paul G. Walfish^**, Chris J. Brandl, and Joe S. Mymryk^§§§¶¶

From the Departments of  Microbiology and Immunology, ^§ Pharmacology and Toxicology,  Biochemistry, and ^§§ Oncology, London Regional Cancer Centre, The University of Western Ontario, London, Ontario N6A 4L6 and ^** Samuel Lunenfeld Research Institute of Mount Sinai Hospital and Department of Medicine, Endocrinology Division, University of Toronto Medical School, Toronto, Ontario M5G 1X5, Canada

Expression of the adenovirus E1A protein in the simple eukaryote Saccharomyces cerevisiae inhibits growth. We tested four regions of E1A that alter growth and transcription in mammalian cells for their effects in yeast when expressed as fusions to the Gal4p DNA binding domain. Expression of the N-terminal/conserved region (CR) 1 or CR3, but not of the CR2 or the C-terminal portion of E1A, inhibited yeast growth. Growth inhibition was relieved by deletion of the genes encoding the yGcn5p, Ngg1p, or Spt7p components of the SAGA transcriptional regulatory complex, but not the Ahc1p component of the related ADA complex, indicating that the N-terminal/CR1 and CR3 regions of E1A target the SAGA complex independently. Expression of the pCAF acetyltransferase, a mammalian homologue of yGcn5p, also suppressed growth inhibition by either portion of E1A. Furthermore, the N-terminal 29 residues and the CR3 portion of E1A interacted independently with yGcn5p and pCAF in vitro. Thus, two separate regions of E1A target the yGcn5p component of the SAGA transcriptional activation complex. A subregion of the N-terminal/CR1 fragment spanning residues 30-69 within CR1 also inhibited yeast growth in a SAGA-dependent fashion. However, this region did not interact with yGcn5p or pCAF, suggesting that it makes a third contact with another SAGA component. Our results provide a new model system to elucidate mechanisms by which E1A and the SAGA complex regulate transcription and growth.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				P. Pelka, J. N. G. Ablack, J. Torchia, A. S. Turnell, R. J. A. Grand, and J. S. Mymryk Transcriptional control by adenovirus E1A conserved region 3 via p300/CBP Nucleic Acids Res., March 1, 2009; 37(4): 1095 - 1106. [Abstract] [Full Text] [PDF]

				V. Stribinskis, H.-C. Heyman, S. R. Ellis, M. C. Steffen, and N. C. Martin Rpm2p, a Component of Yeast Mitochondrial RNase P, Acts as a Transcriptional Activator in the Nucleus Mol. Cell. Biol., August 1, 2005; 25(15): 6546 - 6558. [Abstract] [Full Text] [PDF]

				M. Rasti, R. J. A. Grand, J. S. Mymryk, P. H. Gallimore, and A. S. Turnell Recruitment of CBP/p300, TATA-Binding Protein, and S8 to Distinct Regions at the N Terminus of Adenovirus E1A J. Virol., May 1, 2005; 79(9): 5594 - 5605. [Abstract] [Full Text] [PDF]

				B. A. Burkhart, P. B. Hebbar, K. W. Trotter, and T. K. Archer Chromatin-dependent E1A Activity Modulates NF-{kappa}B RelA-mediated Repression of Glucocorticoid Receptor-dependent Transcription J. Biol. Chem., February 25, 2005; 280(8): 6349 - 6358. [Abstract] [Full Text] [PDF]

				X. Meng, Y.-F. Yang, X. Cao, M. V. Govindan, M. Shuen, A. N. Hollenberg, J. S. Mymryk, and P. G. Walfish Cellular Context of Coregulator and Adaptor Proteins Regulates Human Adenovirus 5 Early Region 1A-Dependent Gene Activation by the Thyroid Hormone Receptor Mol. Endocrinol., June 1, 2003; 17(6): 1095 - 1105. [Abstract] [Full Text] [PDF]