JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M201497200 on June 12, 2002

J. Biol. Chem., Vol. 277, Issue 34, 31115-31123, August 23, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/34/31115    most recent
M201497200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kedar, P. S.
Right arrow Articles by Wilson, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kedar, P. S.
Right arrow Articles by Wilson, S. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Direct Interaction between Mammalian DNA Polymerase beta  and Proliferating Cell Nuclear Antigen*

Padmini S. KedarDagger , Soon-Jong Kim§, Anthony Robertson, Esther HouDagger , Rajendra PrasadDagger , Julie K. HortonDagger , and Samuel H. WilsonDagger ||

From the Dagger  Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709

Proliferating cell nuclear antigen (PCNA) plays an essential role in nucleic acid metabolism as a component of the DNA replication and DNA repair machinery. As such, PCNA interacts with many proteins that have a sequence motif termed the PCNA interacting motif (PIM) and also with proteins lacking a PIM. Three regions in human and rat DNA polymerases beta  (beta -pol) that resemble the consensus PIM were identified, and we show here that beta -polymerase and PCNA can form a complex both in vitro and in vivo. Immunoprecipitation experiments, yeast two-hybrid analysis, and overlay binding assays were used to examine the interaction between the two proteins. Competition experiments with synthetic PIM-containing peptides suggested the importance of a PIM in the interaction, and studies of a beta -polymerase PIM mutant, H222A/F223A, demonstrated that this alteration blocked the interaction with PCNA. The results indicate that at least one of the PIM-like sequences in beta -polymerase appears to be a functional PIM and was required in the interaction between beta -polymerase and PCNA.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ On sabbatical leave from the Dept. of Chemistry, Mokpo National University, Muan, Korea.

Present address: Stowers Inst. for Medical Research, Kansas City, MO 64110.

|| To whom correspondence should be addressed: NIEHS, National Institutes of Health, 111 T.W. Alexander Dr., Research Triangle Park, NC 27709. Tel.: 919-541-3267; Fax: 919-541-3592; E-mail: wilson5@niehs.nih.gov.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
Y. Liu, R. Prasad, W. A. Beard, P. S. Kedar, E. W. Hou, D. D. Shock, and S. H. Wilson
Coordination of Steps in Single-nucleotide Base Excision Repair Mediated by Apurinic/Apyrimidinic Endonuclease 1 and DNA Polymerase beta
J. Biol. Chem., May 4, 2007; 282(18): 13532 - 13541.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
N. El-Andaloussi, T. Valovka, M. Toueille, P. O. Hassa, P. Gehrig, M. Covic, U. Hubscher, and M. O. Hottiger
Methylation of DNA polymerase {beta} by protein arginine methyltransferase 1 regulates its binding to proliferating cell nuclear antigen
FASEB J, January 1, 2007; 21(1): 26 - 34.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. K. Braithwaite, P. S. Kedar, L. Lan, Y. Y. Polosina, K. Asagoshi, V. P. Poltoratsky, J. K. Horton, H. Miller, G. W. Teebor, A. Yasui, et al.
DNA Polymerase {lambda} Protects Mouse Fibroblasts against Oxidative DNA Damage and Is Recruited to Sites of DNA Damage/Repair
J. Biol. Chem., September 9, 2005; 280(36): 31641 - 31647.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S.-H. Hsu, H.-M. Hsieh-Li, H.-Y. Huang, P.-H. Huang, and H. Li
bHLH-zip Transcription Factor Spz1 Mediates Mitogen-Activated Protein Kinase Cell Proliferation, Transformation, and Tumorigenesis
Cancer Res., May 15, 2005; 65(10): 4041 - 4050.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. K. Braithwaite, R. Prasad, D. D. Shock, E. W. Hou, W. A. Beard, and S. H. Wilson
DNA Polymerase {lambda} Mediates a Back-up Base Excision Repair Activity in Extracts of Mouse Embryonic Fibroblasts
J. Biol. Chem., May 6, 2005; 280(18): 18469 - 18475.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. K. Horton, D. F. Stefanick, J. M. Naron, P. S. Kedar, and S. H. Wilson
Poly(ADP-ribose) Polymerase Activity Prevents Signaling Pathways for Cell Cycle Arrest after DNA Methylating Agent Exposure
J. Biol. Chem., April 22, 2005; 280(16): 15773 - 15785.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Narayan, A. S. Jaiswal, and R. Balusu
Tumor Suppressor APC Blocks DNA Polymerase {beta}-dependent Strand Displacement Synthesis during Long Patch but Not Short Patch Base Excision Repair and Increases Sensitivity to Methylmethane Sulfonate
J. Biol. Chem., February 25, 2005; 280(8): 6942 - 6949.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Pascucci, M. T. Russo, M. Crescenzi, M. Bignami, and E. Dogliotti
The accumulation of MMS-induced single strand breaks in G1 phase is recombinogenic in DNA polymerase {beta} defective mammalian cells
Nucleic Acids Res., January 12, 2005; 33(1): 280 - 288.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Toueille, N. El-Andaloussi, I. Frouin, R. Freire, D. Funk, I. Shevelev, E. Friedrich-Heineken, G. Villani, M. O. Hottiger, and U. Hubscher
The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase {beta} and increases its DNA substrate utilisation efficiency: implications for DNA repair
Nucleic Acids Res., June 22, 2004; 32(11): 3316 - 3324.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Wong and B. Demple
Modulation of the 5'-Deoxyribose-5-phosphate Lyase and DNA Synthesis Activities of Mammalian DNA Polymerase {beta} by Apurinic/Apyrimidinic Endonuclease 1
J. Biol. Chem., June 11, 2004; 279(24): 25268 - 25275.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
L. Li, S. H. Berger, and M. D. Wyatt
Involvement of base excision repair in response to therapy targeted at thymidylate synthase
Mol. Cancer Ther., June 1, 2004; 3(6): 747 - 753.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. W. Sobol, M. Kartalou, K. H. Almeida, D. F. Joyce, B. P. Engelward, J. K. Horton, R. Prasad, L. D. Samson, and S. H. Wilson
Base Excision Repair Intermediates Induce p53-independent Cytotoxic and Genotoxic Responses
J. Biol. Chem., October 10, 2003; 278(41): 39951 - 39959.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Wong, M. S. DeMott, and B. Demple
Modulation of the 3'->5'-Exonuclease Activity of Human Apurinic Endonuclease (Ape1) by Its 5'-incised Abasic DNA Product
J. Biol. Chem., September 19, 2003; 278(38): 36242 - 36249.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
G. Maga and U. Hubscher
Proliferating cell nuclear antigen (PCNA): a dancer with many partners
J. Cell Sci., August 1, 2003; 116(15): 3051 - 3060.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. A. Harrigan, P. L. Opresko, C. von Kobbe, P. S. Kedar, R. Prasad, S. H. Wilson, and V. A. Bohr
The Werner Syndrome Protein Stimulates DNA Polymerase {beta} Strand Displacement Synthesis via Its Helicase Activity
J. Biol. Chem., June 13, 2003; 278(25): 22686 - 22695.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Maga, G. Villani, K. Ramadan, I. Shevelev, N. T. Le Gac, L. Blanco, G. Blanca, S. Spadari, and U. Hubscher
Human DNA Polymerase lambda Functionally and Physically Interacts with Proliferating Cell Nuclear Antigen in Normal and Translesion DNA Synthesis
J. Biol. Chem., December 6, 2002; 277(50): 48434 - 48440.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.