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J. Biol. Chem., Vol. 277, Issue 34, 31207-31213, August 23, 2002
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From the Department of Biomedical Sciences, University of Illinois,
College of Medicine, Rockford, Illinois 61107
A homologue of the mammalian translationally
controlled tumor protein (TCTP) was cloned from the human parasite
Schistosoma mansoni (SmTCTP). Sequence analysis showed
that SmTCTP differed from other reported TCTPs in having only one
signature sequence. Subsequently, SmTCTP was cloned in a T7 expression
system and expressed as a histidine-tagged fusion protein.
Recombinant SmTCTP (rSmTCTP) has a molecular mass of ~23 kDa with the
histidine tag. Further analysis showed that SmTCTP transcripts and
protein are expressed in all life cycle stages of the parasite within
the vertebrate hosts. Interestingly, antibodies to SmTCTP were present in the sera of mice 9 weeks after infection with S. mansoni. Characterization studies showed that rSmTCTP is a
calcium-binding protein that can cause histamine release from
basophil/mast cells and induce eosinophil infiltration. These
findings suggest that SmTCTP may have an important role in the
development of allergic inflammatory responses associated with
schistosomiasis and may be a target for new drug development.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF358139.
Cloning and Characterization of a Calcium-binding,
Histamine-releasing Protein from Schistosoma mansoni*
,
*
This work was supported by National Institutes of Health
Grant AI 39066 (to K. R.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Current address: Biomedical Engineering Dept., Northwestern
University, Evanston, IL 60208.
§
To whom correspondence should be addressed: Dept. of Biomedical
Sciences, College of Medicine, University of Illinois, Rockford, IL
61107. Tel.: 815-395-5696, Fax: 815-395-5666; E-mail:
ramswamy@uic.edu.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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