JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M200755200 on April 9, 2002

J. Biol. Chem., Vol. 277, Issue 35, 31291-31302, August 30, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/35/31291    most recent
M200755200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fessler, M. B.
Right arrow Articles by Worthen, G. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fessler, M. B.
Right arrow Articles by Worthen, G. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

A Genomic and Proteomic Analysis of Activation of the Human Neutrophil by Lipopolysaccharide and Its Mediation by p38 Mitogen-activated Protein Kinase*

Michael B. FesslerDagger §, Kenneth C. Malcolm§, Mark William Duncan, and G. Scott WorthenDagger §||

From the Dagger  Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Health Sciences Center, the § Department of Medicine, National Jewish Medical and Research Center, and the  Biochemical Mass Spectrometry Facility, School of Pharmacy, University of Colorado Health Sciences Center, Denver, Colorado 80262

Bacterial lipopolysaccharide (LPS) evokes several functional responses in the neutrophil that contribute to innate immunity. Although certain responses, such as adhesion and synthesis of tumor necrosis factor-alpha , are inhibited by pretreatment with an inhibitor of p38 mitogen-activated protein kinase, others, such as actin assembly, are unaffected. The aim of the present study was to investigate the changes in neutrophil gene transcription and protein expression following lipopolysaccharide exposure and to establish their dependence on p38 signaling. Microarray analysis indicated expression of 13% of the 7070 Affymetrix gene set in nonstimulated neutrophils, and LPS up-regulation of 100 distinct genes, including cytokines and chemokines, signaling molecules, and regulators of transcription. Proteomic analysis yielded a separate list of up-regulated modulators of inflammation, signaling molecules, and cytoskeletal proteins. Poor concordance between mRNA transcript and protein expression changes was noted. Pretreatment with the p38 inhibitor SB203580 attenuated 23% of LPS-regulated genes and 18% of LPS-regulated proteins by >= 40%. This study indicates that p38 plays a selective role in regulation of neutrophil transcripts and proteins following lipopolysaccharide exposure, clarifies that several of the effects of lipopolysaccharide are post-transcriptional and post-translational, and identifies several proteins not previously reported to be involved in the innate immune response.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed: Dept. of Medicine, D403, Neustadt Bldg., National Jewish Medical and Research Center, 1400 Jackson St., Denver, CO 80206. Tel.: 303-398-1171; Fax: 303-398-1381; E-mail: worthens@njc.org.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
K. Smoak, J. Madenspacher, S. Jeyaseelan, B. Williams, D. Dixon, K. R. Poch, J. A. Nick, G. S. Worthen, and M. B. Fessler
Effects of Liver X Receptor Agonist Treatment on Pulmonary Inflammation and Host Defense
J. Immunol., March 1, 2008; 180(5): 3305 - 3312.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Tamassia, V. Le Moigne, F. Calzetti, M. Donini, S. Gasperini, T. Ear, A. Cloutier, F. O. Martinez, M. Fabbri, M. Locati, et al.
The MYD88-Independent Pathway Is Not Mobilized in Human Neutrophils Stimulated via TLR4
J. Immunol., June 1, 2007; 178(11): 7344 - 7356.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. D. Coldren, J. A. Nick, K. R. Poch, M. D. Woolum, B. W. Fouty, J. M. O'Brien, M. P. Gruber, M. R. Zamora, D. Svetkauskaite, D. A. Richter, et al.
Functional and genomic changes induced by alveolar transmigration in human neutrophils
Am J Physiol Lung Cell Mol Physiol, December 1, 2006; 291(6): L1267 - L1276.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Fortin, D. Harbour, M. Fernandes, P. Borgeat, and S. Bourgoin
Differential expression of adenosine receptors in human neutrophils: up-regulation by specific Th1 cytokines and lipopolysaccharide
J. Leukoc. Biol., March 1, 2006; 79(3): 574 - 585.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
K. A. Conrads, M. Yi, K. A. Simpson, D. A. Lucas, C. E. Camalier, L.-R. Yu, T. D. Veenstra, R. M. Stephens, T. P. Conrads, and G. R. Beck Jr.
A Combined Proteome and Microarray Investigation of Inorganic Phosphate-induced Pre-osteoblast Cells
Mol. Cell. Proteomics, September 1, 2005; 4(9): 1284 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
M. Komor, S. Guller, C. D. Baldus, S. de Vos, D. Hoelzer, O. G. Ottmann, and W.-K. Hofmann
Transcriptional Profiling of Human Hematopoiesis During In Vitro Lineage-Specific Differentiation
Stem Cells, September 1, 2005; 23(8): 1154 - 1169.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S. Iida, T. Kohro, T. Kodama, S. Nagata, and R. Fukunaga
Identification of CCR2, flotillin, and gp49B genes as new G-CSF targets during neutrophilic differentiation
J. Leukoc. Biol., August 1, 2005; 78(2): 481 - 490.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
T. Sparre, M. R. Larsen, P. E. Heding, A. E. Karlsen, O. N. Jensen, and F. Pociot
Unraveling the Pathogenesis of Type 1 Diabetes with Proteomics: Present And Future Directions
Mol. Cell. Proteomics, April 1, 2005; 4(4): 441 - 457.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
R. D. Fannin, J. T. Auman, M. E. Bruno, S. O. Sieber, S. M. Ward, C. J. Tucker, B. A. Merrick, and R. S. Paules
Differential gene expression profiling in whole blood during acute systemic inflammation in lipopolysaccharide-treated rats
Physiol Genomics, March 21, 2005; 21(1): 92 - 104.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
B. A. Wetmore and B. A. Merrick
Invited Review: Toxicoproteomics: Proteomics Applied to Toxicology and Pathology
Toxicol Pathol, October 1, 2004; 32(6): 619 - 642.
[Abstract] [PDF]

Home page
 Physiol. GenomicsHome page
I. E. Konstantinov, S. Arab, R. K. Kharbanda, J. Li, M. M. H. Cheung, V. Cherepanov, G. P. Downey, P. P. Liu, E. Cukerman, J. G. Coles, et al.
The remote ischemic preconditioning stimulus modifies inflammatory gene expression in humans
Physiol Genomics, September 16, 2004; 19(1): 143 - 150.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
C. C. Yost, M. M. Denis, S. Lindemann, F. J. Rubner, G. K. Marathe, M. Buerke, T. M. McIntyre, A. S. Weyrich, and G. A. Zimmerman
Activated Polymorphonuclear Leukocytes Rapidly Synthesize Retinoic Acid Receptor-{alpha}: A Mechanism for Translational Control of Transcriptional Events
J. Exp. Med., September 7, 2004; 200(5): 671 - 680.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T.-T. Wang, F. P. Nestel, V. Bourdeau, Y. Nagai, Q. Wang, J. Liao, L. Tavera-Mendoza, R. Lin, J. H. Hanrahan, S. Mader, et al.
Cutting Edge: 1,25-Dihydroxyvitamin D3 Is a Direct Inducer of Antimicrobial Peptide Gene Expression
J. Immunol., September 1, 2004; 173(5): 2909 - 2912.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. A. Whitsett, C. J. Bachurski, K. C. Barnes, P. A. Bunn Jr., L. M. Case, D. N. Cook, D. Crooks, M. W. Duncan, L. Dwyer-Nield, R. C. Elston, et al.
Functional Genomics of Lung Disease
Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2/S1): S1 - S81.
[Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
O. Loseva and Y. Engstrom
Analysis of Signal-dependent Changes in the Proteome of Drosophila Blood Cells During an Immune Response
Mol. Cell. Proteomics, August 1, 2004; 3(8): 796 - 808.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Theilgaard-Monch, S. Knudsen, P. Follin, and N. Borregaard
The Transcriptional Activation Program of Human Neutrophils in Skin Lesions Supports Their Important Role in Wound Healing
J. Immunol., June 15, 2004; 172(12): 7684 - 7693.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. W. Lindemann, C. C. Yost, M. M. Denis, T. M. McIntyre, A. S. Weyrich, and G. A. Zimmerman
Neutrophils alter the inflammatory milieu by signal-dependent translation of constitutive messenger RNAs
PNAS, May 4, 2004; 101(18): 7076 - 7081.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Y. Koga, A. Matsuzaki, A. Suminoe, H. Hattori, and T. Hara
Neutrophil-Derived TNF-Related Apoptosis-Inducing Ligand (TRAIL): A Novel Mechanism of Antitumor Effect by Neutrophils
Cancer Res., February 1, 2004; 64(3): 1037 - 1043.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
X. Zhang, Y. Kluger, Y. Nakayama, R. Poddar, C. Whitney, A. DeTora, S. M. Weissman, and P. E. Newburger
Gene expression in mature neutrophils: early responses to inflammatory stimuli
J. Leukoc. Biol., February 1, 2004; 75(2): 358 - 372.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M. Huber, I. Bahr, J. R. Kratzschmar, A. Becker, E.-C. Muller, P. Donner, H.-D. Pohlenz, M. R. Schneider, and A. Sommer
Comparison of Proteomic and Genomic Analyses of the Human Breast Cancer Cell Line T47D and the Antiestrogen-resistant Derivative T47D-r
Mol. Cell. Proteomics, January 1, 2004; 3(1): 43 - 55.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
H. Suttmann, N. Lehan, A. Bohle, and S. Brandau
Stimulation of Neutrophil Granulocytes with Mycobacterium bovis Bacillus Calmette-Guerin Induces Changes in Phenotype and Gene Expression and Inhibits Spontaneous Apoptosis
Infect. Immun., August 1, 2003; 71(8): 4647 - 4656.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Pellagatti, D. Vetrie, C. F. Langford, S. Gama, H. Eagleton, J. S. Wainscoat, and J. Boultwood
Gene Expression Profiling in Polycythemia Vera Using cDNA Microarray Technology
Cancer Res., July 15, 2003; 63(14): 3940 - 3944.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. El Bekay, M. Alvarez, J. Monteseirin, G. Alba, P. Chacon, A. Vega, J. Martin-Nieto, J. Jimenez, E. Pintado, F. J. Bedoya, et al.
Oxidative stress is a critical mediator of the angiotensin II signal in human neutrophils: involvement of mitogen-activated protein kinase, calcineurin, and the transcription factor NF-{kappa}B
Blood, July 15, 2003; 102(2): 662 - 671.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. M. Milner and A. J. Day
TSG-6: a multifunctional protein associated with inflammation
J. Cell Sci., May 15, 2003; 116(10): 1863 - 1873.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. C. Malcolm and G. S. Worthen
Lipopolysaccharide Stimulates p38-dependent Induction of Antiviral Genes in Neutrophils Independently of Paracrine Factors
J. Biol. Chem., April 25, 2003; 278(18): 15693 - 15701.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. C. Malcolm, P. G. Arndt, E. J. Manos, D. A. Jones, and G. S. Worthen
Microarray analysis of lipopolysaccharide-treated human neutrophils
Am J Physiol Lung Cell Mol Physiol, April 1, 2003; 284(4): L663 - L670.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
L. AUJAME, N. BURDIN, and M. VICARI
How Microarrays Can Improve Our Understanding of Immune Responses and Vaccine Development
Ann. N.Y. Acad. Sci., December 1, 2002; 975(1): 1 - 23.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Sitia, M. Isogawa, K. Kakimi, S. F. Wieland, F. V. Chisari, and L. G. Guidotti
Depletion of neutrophils blocks the recruitment of antigen-nonspecific cells into the liver without affecting the antiviral activity of hepatitis B virus-specific cytotoxic T lymphocytes
PNAS, October 15, 2002; 99(21): 13717 - 13722.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.