Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M204420200 on June 14, 2002

J. Biol. Chem., Vol. 277, Issue 36, 32445-32452, September 6, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/36/32445    most recent
M204420200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Longhurst, C. M.
Right arrow Articles by Jennings, L. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Longhurst, C. M.
Right arrow Articles by Jennings, L. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Chinese Hamster Ovary Cell Motility to Fibronectin Is Modulated by the Second Extracellular Loop of CD9
IDENTIFICATION OF A PUTATIVE FIBRONECTIN BINDING SITE*

Celia M. LonghurstDagger , Jonathan D. JacobsDagger , Melanie M. WhiteDagger , Joseph T. Crossno Jr.Dagger , Deborah A. FitzgeraldDagger , Jianxong BaoDagger , Thomas J. FitzgeraldDagger , Rajendra RaghowDagger §||, and Lisa K. JenningsDagger **

From the Dagger  Vascular Biology Center of Excellence and the § Department of Pharmacology, University of Tennessee Health Science Center and the  Veterans Administration Medical Center, Memphis, Tennessee 38163

CD9, a member of the tetraspanin family of proteins, is characterized by four transmembrane domains and two extracellular loops. Surface expression of CD9 on Chinese hamster ovary (CHO) cells dramatically enhances spreading and motility on fibronectin. To elucidate the mechanistic basis of CD9-fibronectin interaction, binding to fibronectin was investigated using purified and recombinant forms of CD9. The affinity of fibronectin for CD9 in enzyme-linked immunosorbent assay was 81 ± 25 nM. The binding of fibronectin to immobilized CD9 was enhanced by Ca2+ ions. Protein binding and peptide competition studies demonstrated that peptide 6 derived from CD9 extracellular loop 2 (amino acids 168-192) contained part of the fibronectin-binding domain. Additionally, enhanced adhesion of CD9-CHO-B2 cells to fibronectin was significantly reduced by peptide 6. CD9-CHO cells had a 5-fold increase in motility to fibronectin as compared with mock-transfected controls, an effect that correlated with CD9 cell surface density. Truncation of CD9 extracellular loop 2 and peptide 6 caused inhibition of CD9-CHO cell motility to fibronectin. Deletion of CD9 extracellular loop 1 had no significant effect on CHO cell motility. These findings demonstrate a critical role for CD9 extracellular loop 2 in cell motility to fibronectin and clarify the mechanism by which CD9-fibronectin interaction modulates cell adhesion and motility.

* This work was supported in part by The Vascular Biology Center of Excellence Support Fund, the NHLBI of the National Institutes of Health (Grant HL53514), the American Heart Association (Grant 0230187N), and the Department of Veterans Affairs (DVA).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| A senior research career scientist of DVA.

** To whom correspondence should be addressed: Vascular Biology Center of Excellence, University of Tennessee Health Science Center, 956 Court Ave., Rm. H300, Memphis, TN 38163. Tel.: 901-448-5067; Fax: 901-448-7181; E-mail: ljennings@utmem.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
R. Bari, Y. H. Zhang, F. Zhang, N. X. Wang, C. S. Stipp, J. J. Zheng, and X. A. Zhang
Transmembrane Interactions Are Needed for KAI1/CD82-Mediated Suppression of Cancer Invasion and Metastasis
Am. J. Pathol., February 1, 2009; 174(2): 647 - 660.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
I. Imhof, W. J. Gasper, and R. Derynck
Association of tetraspanin CD9 with transmembrane TGF{alpha} confers alterations in cell-surface presentation of TGF{alpha} and cytoskeletal organization
J. Cell Sci., July 1, 2008; 121(13): 2265 - 2274.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. M. Segura, A. Garnier, M. R. Di Falco, G. Whissell, A. Meneses-Acosta, N. Arcand, and A. Kamen
Identification of Host Proteins Associated with Retroviral Vector Particles by Proteomic Analysis of Highly Purified Vector Preparations
J. Virol., February 1, 2008; 82(3): 1107 - 1117.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Y. Saito, I. Tachibana, Y. Takeda, H. Yamane, P. He, M. Suzuki, S. Minami, T. Kijima, M. Yoshida, T. Kumagai, et al.
Absence of CD9 Enhances Adhesion-Dependent Morphologic Differentiation, Survival, and Matrix Metalloproteinase-2 Production in Small Cell Lung Cancer Cells
Cancer Res., October 1, 2006; 66(19): 9557 - 9565.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. A. Cook, C. M. Longhurst, S. Grgurevich, S. Cholera, J. T. Crossno Jr, and L. K. Jennings
Identification of CD9 extracellular domains important in regulation of CHO cell adhesion to fibronectin and fibronectin pericellular matrix assembly
Blood, December 15, 2002; 100(13): 4502 - 4511.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement