Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M204065200 on June 21, 2002

J. Biol. Chem., Vol. 277, Issue 36, 32640-32649, September 6, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/36/32640    most recent
M204065200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, Z.-M.
Right arrow Articles by Garrard, W. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, Z.-M.
Right arrow Articles by Garrard, W. T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Chromatin Structural Analyses of the Mouse Igkappa Gene Locus Reveal New Hypersensitive Sites Specifying a Transcriptional Silencer and Enhancer*

Zhi-Mei Liu, Julia B. George-Raizen, Shuyu Li, Katherine C. Meyers, Mee Young Chang, and William T. GarrardDagger

From the Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9148

To identify new regulatory elements within the mouse Igkappa locus, we have mapped DNase I hypersensitive sites (HSs) in the chromatin of B cell lines arrested at different stages of differentiation. We have focused on two regions encompassing 50 kilobases suspected to contain new regulatory elements based on our previous high level expression results with yeast artificial chromosome-based mouse Igkappa transgenes. This approach has revealed a cluster of HSs within the 18-kilobase intervening sequence, which we cloned and sequenced in its entirety, between the Vkappa gene closest to the Jkappa region. These HSs exhibit pro/pre-B cell-specific transcriptional silencing of a Vkappa gene promoter in transient transfection assays. We also identified a plasmacytoma cell-specific HS in the far downstream region of the locus, which in analogous transient transfection assays proved to be a powerful transcriptional enhancer. Deletional analyses reveal that for each element multiple DNA segments cooperate to achieve either silencing or enhancement. The enhancer sequence is conserved in the human Igkappa gene locus, including NF-kappa B and E-box sites that are important for the activity. In summary, our results pinpoint the locations of presumptive regulatory elements for future knockout studies to define their functional roles in the native locus.

* This investigation was supported by National Institutes of Health Grant GM29935 and Robert A. Welch Foundation Grant I-823 (to W. T. G.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF513925 and AF513926.

Dagger To whom correspondence should be addressed: Dept. of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9148. Tel.: 214-648-1924; Fax: 214-648-1915; E-mail: william.garrard@utsouthwestern.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
Y. Xiang and W. T. Garrard
The Downstream Transcriptional Enhancer, Ed, Positively Regulates Mouse Ig{kappa} Gene Expression and Somatic Hypermutation
J. Immunol., May 15, 2008; 180(10): 6725 - 6732.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. P. Fitzsimmons, R. M. Bernstein, E. E. Max, J. A. Skok, and M. A. Shapiro
Dynamic Changes in Accessibility, Nuclear Positioning, Recombination, and Transcription at the Ig{kappa} Locus
J. Immunol., October 15, 2007; 179(8): 5264 - 5273.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. A. Inlay, H. H. Gao, V. H. Odegard, T. Lin, D. G. Schatz, and Y. Xu
Roles of the Ig {kappa} Light Chain Intronic and 3' Enhancers in Igk Somatic Hypermutation
J. Immunol., July 15, 2006; 177(2): 1146 - 1151.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
M. A. Inlay, T. Lin, H. H. Gao, and Y. Xu
Critical roles of the immunoglobulin intronic enhancers in maintaining the sequential rearrangement of IgH and Igk loci
J. Exp. Med., July 10, 2006; 203(7): 1721 - 1732.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
V. Volgina, P.-C. Yam, and K. L. Knight
A negative regulatory element in the rabbit 3'IgH chromosomal region
Int. Immunol., August 1, 2005; 17(8): 973 - 982.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Z. Liu and W. T. Garrard
Long-Range Interactions between Three Transcriptional Enhancers, Active V{kappa} Gene Promoters, and a 3' Boundary Sequence Spanning 46 Kilobases
Mol. Cell. Biol., April 15, 2005; 25(8): 3220 - 3231.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
M. A. Inlay, H. Tian, T. Lin, and Y. Xu
Important Roles for E Protein Binding Sites within the Immunoglobulin {kappa} Chain Intronic Enhancer in Activating V{kappa} J{kappa} Rearrangement
J. Exp. Med., November 1, 2004; 200(9): 1205 - 1211.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement