JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M202120200 on June 25, 2002

J. Biol. Chem., Vol. 277, Issue 36, 32753-32759, September 6, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/36/32753    most recent
M202120200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Doe, C. L.
Right arrow Articles by Whitby, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doe, C. L.
Right arrow Articles by Whitby, M. C.

Mus81-Eme1 and Rqh1 Involvement in Processing Stalled and Collapsed Replication Forks*

Claudette L. Doe, Jong Sook Ahn, Julie Dixon, and Matthew C. WhitbyDagger

From the Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom

The processing of stalled replication forks and the repair of collapsed replication forks are essential functions in all organisms. In fission yeast DNA junctions at stalled replication forks appear to be processed by either the Rqh1 DNA helicase or Mus81-Eme1 endonuclease. Accordingly, we show that the hypersensitivity to agents that cause replication fork stalling of mus81, eme1, and rqh1 mutants is suppressed by a Holliday junction resolvase (RusA), as is the synthetic lethality of a mus81- rqh1- double mutant. Recombinant Mus81-Eme1, purified from Escherichia coli, readily cleaves replication fork structures but cleaves synthetic Holliday junctions relatively poorly in vitro. From these data we propose that Mus81-Eme1 can process stalled replication forks before they have regressed to form a Holliday junction. We also implicate Mus81-Eme1 and Rqh1 in the repair of collapsed replication forks. Here Mus81-Eme1 and Rqh1 seem to function on different substrates because RusA can substitute for Mus81-Eme1 but not Rqh1.

* This work was supported by Project Grant 065278/Z/01/Z and by a Senior Research Fellowship from the Wellcome Trust (to M. C. W.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 44-1865-275192; Fax: 44-1865-275297; E-mail: whitby@bioch.ox.ac.uk.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 Genes Dev.Home page
J. H. Chang, J. J. Kim, J. M. Choi, J. H. Lee, and Y. Cho
Crystal structure of the Mus81-Eme1 complex
Genes & Dev., April 15, 2008; 22(8): 1093 - 1106.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. T. Ehmsen and W.-D. Heyer
Saccharomyces cerevisiae Mus81-Mms4 is a catalytic, DNA structure-selective endonuclease
Nucleic Acids Res., April 1, 2008; 36(7): 2182 - 2195.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. R. Taylor and C. H. McGowan
Cleavage mechanism of human Mus81-Eme1 acting on Holliday-junction structures
PNAS, March 11, 2008; 105(10): 3757 - 3762.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
B. Froget, J. Blaisonneau, S. Lambert, and G. Baldacci
Cleavage of Stalled Forks by Fission Yeast Mus81/Eme1 in Absence of DNA Replication Checkpoint
Mol. Biol. Cell, February 1, 2008; 19(2): 445 - 456.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. B. Ansbach, C. Noguchi, I. W. Klansek, M. Heidlebaugh, T. M. Nakamura, and E. Noguchi
RFCCtf18 and the Swi1-Swi3 Complex Function in Separate and Redundant Pathways Required for the Stabilization of Replication Forks to Facilitate Sister Chromatid Cohesion in Schizosaccharomyces pombe
Mol. Biol. Cell, February 1, 2008; 19(2): 595 - 607.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
M. Hashimoto, M. Taniguchi, S. Yoshino, S. Arai, and K. Sato
S Phase-preferential Cre-recombination in Mammalian Cells Revealed by HIV-TAT-PTD-mediated Protein Transduction
J. Biochem., January 1, 2008; 143(1): 87 - 95.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
Y. Nomura, N. Adachi, and H. Koyama
Human Mus81 and FANCB independently contribute to repair of DNA damage during replication
Genes Cells, October 1, 2007; 12(10): 1111 - 1122.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Pamidi, R. Cardoso, A. Hakem, E. Matysiak-Zablocki, A. Poonepalli, L. Tamblyn, B. Perez-Ordonez, M. P. Hande, O. Sanchez, and R. Hakem
Functional Interplay of p53 and Mus81 in DNA Damage Responses and Cancer
Cancer Res., September 15, 2007; 67(18): 8527 - 8535.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. Trowbridge, K. McKim, S. J. Brill, and J. Sekelsky
Synthetic Lethality of Drosophila in the Absence of the MUS81 Endonuclease and the DmBlm Helicase Is Associated With Elevated Apoptosis
Genetics, August 1, 2007; 176(4): 1993 - 2001.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. C. Hope, L. D. Cruzata, A. Duvshani, J. Mitsumoto, M. Maftahi, and G. A. Freyer
Mus81-Eme1-Dependent and -Independent Crossovers Form in Mitotic Cells during Double-Strand Break Repair in Schizosaccharomyces pombe
Mol. Cell. Biol., May 15, 2007; 27(10): 3828 - 3838.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Macmaster, S. Sedelnikova, P. J. Baker, E. L. Bolt, R. G. Lloyd, and J. B. Rafferty
RusA Holliday junction resolvase: DNA complex structure--insights into selectivity and specificity
Nucleic Acids Res., November 14, 2006; 34(19): 5577 - 5584.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Hartung, S. Suer, T. Bergmann, and H. Puchta
The role of AtMUS81 in DNA repair and its genetic interaction with the helicase AtRecQ4A
Nucleic Acids Res., September 11, 2006; 34(16): 4438 - 4448.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. A. Cobb and L. Bjergbaek
RecQ helicases: lessons from model organisms
Nucleic Acids Res., September 10, 2006; 34(15): 4106 - 4114.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Pebernard, J. Wohlschlegel, W. H. McDonald, J. R. Yates III, and M. N. Boddy
The nse5-nse6 dimer mediates DNA repair roles of the smc5-smc6 complex.
Mol. Cell. Biol., March 1, 2006; 26(5): 1617 - 1630.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Tanaka and H. Masai
Stabilization of a Stalled Replication Fork by Concerted Actions of Two Helicases
J. Biol. Chem., February 10, 2006; 281(6): 3484 - 3493.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Hiyama, M. Katsura, T. Yoshihara, M. Ishida, A. Kinomura, T. Tonda, T. Asahara, and K. Miyagawa
Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells
Nucleic Acids Res., February 2, 2006; 34(3): 880 - 892.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. A. Rao, A. M. Fan, L. Meng, C. F. Doe, P. S. North, I. D. Hickson, and Y. Pommier
Phosphorylation of BLM, Dissociation from Topoisomerase III{alpha}, and Colocalization with {gamma}-H2AX after Topoisomerase I-Induced Replication Damage
Mol. Cell. Biol., October 15, 2005; 25(20): 8925 - 8937.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. M. Sheedy, D. Dimitrova, J. K. Rankin, K. L. Bass, K. M. Lee, C. Tapia-Alveal, S. H. Harvey, J. M. Murray, and M. J. O'Connell
Brc1-Mediated DNA Repair and Damage Tolerance
Genetics, October 1, 2005; 171(2): 457 - 468.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Dendouga, H. Gao, D. Moechars, M. Janicot, J. Vialard, and C. H. McGowan
Disruption of Murine Mus81 Increases Genomic Instability and DNA Damage Sensitivity but Does Not Promote Tumorigenesis
Mol. Cell. Biol., September 1, 2005; 25(17): 7569 - 7579.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Saleh-Gohari, H. E. Bryant, N. Schultz, K. M. Parker, T. N. Cassel, and T. Helleday
Spontaneous Homologous Recombination Is Induced by Collapsed Replication Forks That Are Caused by Endogenous DNA Single-Strand Breaks
Mol. Cell. Biol., August 15, 2005; 25(16): 7158 - 7169.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. P. Guy and E. L. Bolt
Archaeal Hel308 helicase targets replication forks in vivo and in vitro and unwinds lagging strands
Nucleic Acids Res., June 30, 2005; 33(11): 3678 - 3690.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. C. Hope, M. Maftahi, and G. A. Freyer
A Postsynaptic Role for Rhp55/57 That Is Responsible for Cell Death in {Delta}rqh1 Mutants Following Replication Arrest in Schizosaccharomyces pombe
Genetics, June 1, 2005; 170(2): 519 - 531.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. Kai, M. N. Boddy, P. Russell, and T. S.-F. Wang
Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress
Genes & Dev., April 15, 2005; 19(8): 919 - 932.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Zhang, S. Sengupta, Q. Yang, S. P. Linke, N. Yanaihara, J. Bradsher, V. Blais, C. H. McGowan, and C. C. Harris
BLM Helicase Facilitates Mus81 Endonuclease Activity in Human Cells
Cancer Res., April 1, 2005; 65(7): 2526 - 2531.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Komori, M. Hidaka, T. Horiuchi, R. Fujikane, H. Shinagawa, and Y. Ishino
Cooperation of the N-terminal Helicase and C-terminal Endonuclease Activities of Archaeal Hef Protein in Processing Stalled Replication Forks
J. Biol. Chem., December 17, 2004; 279(51): 53175 - 53185.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Tomita, T. Kibe, H.-Y. Kang, Y.-S. Seo, M. Uritani, T. Ushimaru, and M. Ueno
Fission Yeast Dna2 Is Required for Generation of the Telomeric Single-Strand Overhang
Mol. Cell. Biol., November 1, 2004; 24(21): 9557 - 9567.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Pebernard, W. H. McDonald, Y. Pavlova, J. R. Yates III, and M. N. Boddy
Nse1, Nse2, and a Novel Subunit of the Smc5-Smc6 Complex, Nse3, Play a Crucial Role in Meiosis
Mol. Biol. Cell, November 1, 2004; 15(11): 4866 - 4876.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. L. Doe, F. Osman, J. Dixon, and M. C. Whitby
DNA repair by a Rad22-Mus81-dependent pathway that is independent of Rhp51
Nucleic Acids Res., October 14, 2004; 32(18): 5570 - 5581.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. Noguchi, C. Noguchi, W. H. McDonald, J. R. Yates III, and P. Russell
Swi1 and Swi3 Are Components of a Replication Fork Protection Complex in Fission Yeast
Mol. Cell. Biol., October 1, 2004; 24(19): 8342 - 8355.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. P. McPherson, B. Lemmers, R. Chahwan, A. Pamidi, E. Migon, E. Matysiak-Zablocki, M. E. Moynahan, J. Essers, K. Hanada, A. Poonepalli, et al.
Involvement of Mammalian Mus81 in Genome Integrity and Tumor Suppression
Science, June 18, 2004; 304(5678): 1822 - 1826.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
R. C.A.M. van Waardenburg, L. A. de Jong, F. van Delft, M. A.J. van Eijndhoven, M. Bohlander, M.-A. Bjornsti, J. Brouwer, and J. H.M. Schellens
Homologous recombination is a highly conserved determinant of the synergistic cytotoxicity between cisplatin and DNA topoisomerase I poisons
Mol. Cancer Ther., April 1, 2004; 3(4): 393 - 402.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. Vengrova and J. Z. Dalgaard
RNase-sensitive DNA modification(s) initiates S. pombe mating-type switching
Genes & Dev., April 1, 2004; 18(7): 794 - 804.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. L. Doe and M. C. Whitby
The involvement of Srs2 in post-replication repair and homologous recombination in fission yeast
Nucleic Acids Res., March 1, 2004; 32(4): 1480 - 1491.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
V. Blais, H. Gao, C. A. Elwell, M. N. Boddy, P.-H. L. Gaillard, P. Russell, and C. H. McGowan
RNA Interference Inhibition of Mus81 Reduces Mitotic Recombination in Human Cells
Mol. Biol. Cell, February 1, 2004; 15(2): 552 - 562.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
N. M. Hollingsworth and S. J. Brill
The Mus81 solution to resolution: generating meiotic crossovers without Holliday junctions
Genes & Dev., January 15, 2004; 18(2): 117 - 125.
[Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. Gao, X.-B. Chen, and C. H. McGowan
Mus81 Endonuclease Localizes to Nucleoli and to Regions of DNA Damage in Human S-phase Cells
Mol. Biol. Cell, December 1, 2003; 14(12): 4826 - 4834.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. Noguchi, C. Noguchi, L.-L. Du, and P. Russell
Swi1 Prevents Replication Fork Collapse and Controls Checkpoint Kinase Cds1
Mol. Cell. Biol., November 1, 2003; 23(21): 7861 - 7874.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Ueno, T. Nakazaki, Y. Akamatsu, K. Watanabe, K. Tomita, H. D. Lindsay, H. Shinagawa, and H. Iwasaki
Molecular Characterization of the Schizosaccharomyces pombe nbs1+ Gene Involved in DNA Repair and Telomere Maintenance
Mol. Cell. Biol., September 15, 2003; 23(18): 6553 - 6563.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. N. Boddy, P. Shanahan, W. H. McDonald, A. Lopez-Girona, E. Noguchi, J. R. Yates III, and P. Russell
Replication Checkpoint Kinase Cds1 Regulates Recombinational Repair Protein Rad60
Mol. Cell. Biol., August 15, 2003; 23(16): 5939 - 5946.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Tomita, A. Matsuura, T. Caspari, A. M. Carr, Y. Akamatsu, H. Iwasaki, K.-i. Mizuno, K. Ohta, M. Uritani, T. Ushimaru, et al.
Competition between the Rad50 Complex and the Ku Heterodimer Reveals a Role for Exo1 in Processing Double-Strand Breaks but Not Telomeres
Mol. Cell. Biol., August 1, 2003; 23(15): 5186 - 5197.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Ciccia, A. Constantinou, and S. C. West
Identification and Characterization of the Human Mus81-Eme1 Endonuclease
J. Biol. Chem., June 27, 2003; 278(27): 25172 - 25178.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
T. de los Santos, N. Hunter, C. Lee, B. Larkin, J. Loidl, and N. M. Hollingsworth
The Mus81/Mms4 Endonuclease Acts Independently of Double-Holliday Junction Resolution to Promote a Distinct Subset of Crossovers During Meiosis in Budding Yeast
Genetics, May 1, 2003; 164(1): 81 - 94.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. C. Whitby, F. Osman, and J. Dixon
Cleavage of Model Replication Forks by Fission Yeast Mus81-Eme1 and Budding Yeast Mus81-Mms4
J. Biol. Chem., February 21, 2003; 278(9): 6928 - 6935.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Fabre, A. Chan, W.-D. Heyer, and S. Gangloff
Alternate pathways involving Sgs1/Top3, Mus81/ Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication
PNAS, December 24, 2002; 99(26): 16887 - 16892.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
L. S. Symington
Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair
Microbiol. Mol. Biol. Rev., December 1, 2002; 66(4): 630 - 670.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. R. Vance and T. E. Wilson
Yeast Tdp1 and Rad1-Rad10 function as redundant pathways for repairing Top1 replicative damage
PNAS, October 15, 2002; 99(21): 13669 - 13674.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.